Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin
This study aimed to investigate the biotransformation capabilities of a hydroquinone-tolerant Digitalis purpurea cell line (DpHQ) for bioconverting hydroquinone (HQ) into arbutin, a compound with significant therapeutic and cosmetic applications. The research evaluated the influence of various HQ co...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/1/84 |
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| author | Carmen Elena Pop Ana Coste Ana-Maria Vlase Constantin Deliu Mircea Tămaș Tibor Casian Laurian Vlase |
| author_facet | Carmen Elena Pop Ana Coste Ana-Maria Vlase Constantin Deliu Mircea Tămaș Tibor Casian Laurian Vlase |
| author_sort | Carmen Elena Pop |
| collection | DOAJ |
| description | This study aimed to investigate the biotransformation capabilities of a hydroquinone-tolerant Digitalis purpurea cell line (DpHQ) for bioconverting hydroquinone (HQ) into arbutin, a compound with significant therapeutic and cosmetic applications. The research evaluated the influence of various HQ concentrations, feeding protocols, and carbon sources on arbutin bioconversion yield. By using HPLC-MS for the quantification of arbutin in biomass and medium, the study revealed that higher precursor (HQ) concentration led to a more pronounced growth inhibition under single dosing than sequential dosing. At lower sugar (3%) and precursor (4 mM HQ) levels, arbutin predominantly remained within the cells, whereas higher sugar (6%) and HQ (5–6 mM) levels promoted its release into the medium. Arbutin production ranged from 591 mg/L under single dosing to 3049 mg/L with sequential dosing, with the highest yield being achieved with 5 mM HQ in divided doses and 6% glucose. This study holds novelty for being the first to demonstrate the DpHQ’s tolerance to high concentrations of HQ and its efficient capabilities to bioconvert HQ to arbutin, indicating that D. purpurea is equipped with the enzymes required for this process. These aspects highlight its potential as a biotechnological source for arbutin synthesis. |
| first_indexed | 2024-03-08T10:44:06Z |
| format | Article |
| id | doaj.art-2b7b8c568b2f4c6788b41fd88eb8b2f5 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:44:06Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-2b7b8c568b2f4c6788b41fd88eb8b2f52024-01-26T17:20:46ZengMDPI AGLife2075-17292024-01-011418410.3390/life14010084Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into ArbutinCarmen Elena Pop0Ana Coste1Ana-Maria Vlase2Constantin Deliu3Mircea Tămaș4Tibor Casian5Laurian Vlase6Department of Pharmaceutical Industry and Biotechnology, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaInstitute of Biological Research Cluj-Napoca, National Institute for Research and Development in Biological Sciences, 48 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaInstitute of Biological Research Cluj-Napoca, National Institute for Research and Development in Biological Sciences, 48 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaThis study aimed to investigate the biotransformation capabilities of a hydroquinone-tolerant Digitalis purpurea cell line (DpHQ) for bioconverting hydroquinone (HQ) into arbutin, a compound with significant therapeutic and cosmetic applications. The research evaluated the influence of various HQ concentrations, feeding protocols, and carbon sources on arbutin bioconversion yield. By using HPLC-MS for the quantification of arbutin in biomass and medium, the study revealed that higher precursor (HQ) concentration led to a more pronounced growth inhibition under single dosing than sequential dosing. At lower sugar (3%) and precursor (4 mM HQ) levels, arbutin predominantly remained within the cells, whereas higher sugar (6%) and HQ (5–6 mM) levels promoted its release into the medium. Arbutin production ranged from 591 mg/L under single dosing to 3049 mg/L with sequential dosing, with the highest yield being achieved with 5 mM HQ in divided doses and 6% glucose. This study holds novelty for being the first to demonstrate the DpHQ’s tolerance to high concentrations of HQ and its efficient capabilities to bioconvert HQ to arbutin, indicating that D. purpurea is equipped with the enzymes required for this process. These aspects highlight its potential as a biotechnological source for arbutin synthesis.https://www.mdpi.com/2075-1729/14/1/84cell suspensions<i>Digitalis purpurea</i> L.high yield arbutin productionhydroquinone |
| spellingShingle | Carmen Elena Pop Ana Coste Ana-Maria Vlase Constantin Deliu Mircea Tămaș Tibor Casian Laurian Vlase Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin Life cell suspensions <i>Digitalis purpurea</i> L. high yield arbutin production hydroquinone |
| title | Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin |
| title_full | Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin |
| title_fullStr | Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin |
| title_full_unstemmed | Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin |
| title_short | Selection of a <i>Digitalis purpurea</i> Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin |
| title_sort | selection of a i digitalis purpurea i cell line with improved bioconversion capacity of hydroquinone into arbutin |
| topic | cell suspensions <i>Digitalis purpurea</i> L. high yield arbutin production hydroquinone |
| url | https://www.mdpi.com/2075-1729/14/1/84 |
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