Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties
<i>Justicia secunda</i> Vahl. is a traditional medicinal plant in tropical regions, including West Africa. The present study examined the chemical profiles and biological properties of <i>J. secunda</i> extracts obtained with different solvents (dichloromethane, ethyl acetate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/509 |
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| author | Łukasz Świątek Elwira Sieniawska Kouadio Ibrahime Sinan Gokhan Zengin Anastazja Boguszewska Benita Hryć Kouadio Bene Małgorzata Polz-Dacewicz Stefano Dall’Acqua |
| author_facet | Łukasz Świątek Elwira Sieniawska Kouadio Ibrahime Sinan Gokhan Zengin Anastazja Boguszewska Benita Hryć Kouadio Bene Małgorzata Polz-Dacewicz Stefano Dall’Acqua |
| author_sort | Łukasz Świątek |
| collection | DOAJ |
| description | <i>Justicia secunda</i> Vahl. is a traditional medicinal plant in tropical regions, including West Africa. The present study examined the chemical profiles and biological properties of <i>J. secunda</i> extracts obtained with different solvents (dichloromethane, ethyl acetate, methanolic and aqueous: macerated and infused). Chemical components were characterized by liquid chromatography-mass spectrometry (LC-MS), and over 50 compounds were identified, including flavonoids, phenolic acids, and alkaloids. Antioxidant, enzyme inhibitory, cytotoxic, and antiviral properties were selected as biological properties. Total phenolic and flavonoid contents in methanol (58.07 mg gallic acid equivalent (GAE)/g and 13.07 mg rutin equivalent (RE)/g) and water (infused) (36.34 mg GAE/g and 8.52 mg RE/g) were higher than in other extracts. Consistent with the levels of total bioactive components, the methanol and water extracts exhibited stronger antioxidant abilities. However, the dichloromethane and ethyl acetate extracts were more active on α-amylase and α-glucosidase than other extracts. Aqueous extracts exerted selective anticancer properties toward human pharyngeal cancer cell lines, whereas the methanolic extract decreased the human herpesvirus type-1 (HHV-1) infectious titer by 2.16 log and the viral load by 1.21 log. Overall, <i>J. secunda</i> could be considered a multifunctional bioactive raw material in the preparation of potent applications to manage diseases related to oxidative stress, including cancer, diabetes, and Alzheimer’s. |
| first_indexed | 2024-03-11T09:13:48Z |
| format | Article |
| id | doaj.art-2b7bd6d5d94f44f29a24c8da785de6d1 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:13:48Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-2b7bd6d5d94f44f29a24c8da785de6d12023-11-16T18:49:15ZengMDPI AGAntioxidants2076-39212023-02-0112250910.3390/antiox12020509Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic PropertiesŁukasz Świątek0Elwira Sieniawska1Kouadio Ibrahime Sinan2Gokhan Zengin3Anastazja Boguszewska4Benita Hryć5Kouadio Bene6Małgorzata Polz-Dacewicz7Stefano Dall’Acqua8Department of Virology with SARS Laboratory, Medical University of Lublin, Chodzki 1, 20-093 Lublin, PolandDepartment of Natural Products Chemistry, Medical University of Lublin, Chodzki 1, 20-093 Lublin, PolandDepartment of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Virology with SARS Laboratory, Medical University of Lublin, Chodzki 1, 20-093 Lublin, PolandMedicofarma Biotech S.A., Zamenhofa 29, 20-453 Lublin, PolandLaboratoire de Botanique et Phytothérapie, Unité de Formation et de Recherche Sciences de la Nature, Université Nangui Abrogoua, Abidjan 02 BP 801, Côte d’IvoireDepartment of Virology with SARS Laboratory, Medical University of Lublin, Chodzki 1, 20-093 Lublin, PolandDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy<i>Justicia secunda</i> Vahl. is a traditional medicinal plant in tropical regions, including West Africa. The present study examined the chemical profiles and biological properties of <i>J. secunda</i> extracts obtained with different solvents (dichloromethane, ethyl acetate, methanolic and aqueous: macerated and infused). Chemical components were characterized by liquid chromatography-mass spectrometry (LC-MS), and over 50 compounds were identified, including flavonoids, phenolic acids, and alkaloids. Antioxidant, enzyme inhibitory, cytotoxic, and antiviral properties were selected as biological properties. Total phenolic and flavonoid contents in methanol (58.07 mg gallic acid equivalent (GAE)/g and 13.07 mg rutin equivalent (RE)/g) and water (infused) (36.34 mg GAE/g and 8.52 mg RE/g) were higher than in other extracts. Consistent with the levels of total bioactive components, the methanol and water extracts exhibited stronger antioxidant abilities. However, the dichloromethane and ethyl acetate extracts were more active on α-amylase and α-glucosidase than other extracts. Aqueous extracts exerted selective anticancer properties toward human pharyngeal cancer cell lines, whereas the methanolic extract decreased the human herpesvirus type-1 (HHV-1) infectious titer by 2.16 log and the viral load by 1.21 log. Overall, <i>J. secunda</i> could be considered a multifunctional bioactive raw material in the preparation of potent applications to manage diseases related to oxidative stress, including cancer, diabetes, and Alzheimer’s.https://www.mdpi.com/2076-3921/12/2/509<i>Justicia</i>phenolicsantiviralbioactive agentsenzyme inhibitors |
| spellingShingle | Łukasz Świątek Elwira Sieniawska Kouadio Ibrahime Sinan Gokhan Zengin Anastazja Boguszewska Benita Hryć Kouadio Bene Małgorzata Polz-Dacewicz Stefano Dall’Acqua Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties Antioxidants <i>Justicia</i> phenolics antiviral bioactive agents enzyme inhibitors |
| title | Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties |
| title_full | Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties |
| title_fullStr | Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties |
| title_full_unstemmed | Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties |
| title_short | Chemical Characterization of Different Extracts of <i>Justicia secunda</i> Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties |
| title_sort | chemical characterization of different extracts of i justicia secunda i vahl and determination of their anti oxidant anti enzymatic anti viral and cytotoxic properties |
| topic | <i>Justicia</i> phenolics antiviral bioactive agents enzyme inhibitors |
| url | https://www.mdpi.com/2076-3921/12/2/509 |
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