Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei
Trichoderma enzymes that inhibit fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal root rot pathogen Ganoderma philippii. This experiment was aimed to purify and characterize the β-1,3-glucanase of T. reesei. Extracellular β-1,3-glucanase was prod...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2009-09-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/viewFile/282/147 |
| _version_ | 1819055895376560128 |
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| author | SRI WAHYUNI BUDIARTI SITI MUSLIMAH WIDYASTUTI SEBASTIAN MARGINO |
| author_facet | SRI WAHYUNI BUDIARTI SITI MUSLIMAH WIDYASTUTI SEBASTIAN MARGINO |
| author_sort | SRI WAHYUNI BUDIARTI |
| collection | DOAJ |
| description | Trichoderma enzymes that inhibit fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal root rot pathogen Ganoderma philippii. This experiment was aimed to purify and characterize the β-1,3-glucanase of T. reesei. Extracellular β-1,3-glucanase was produced by growing mycoparasite T. reesei isolate T13 in colloidal chitin and sucrose as carbon sources. The enzyme was then purified to its homogeneity by precipitation with ammonium sulfate, followed by gel filtration chromatography and chromatofocusing. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 12% was used to confirm the purity of enzyme at each stage of preparation and to characterize purified protein. The results showed that T. reesei produced at least three extracellular β-1,3-glucanases. Estimation of molecular weight based on SDS-PAGE 12% have three isoform of β-1,3-glucanase were 90 kDa for β-1,3-glucanase-I, 75 kDa for β-1,3-glucanase-II, and 64 kDa for β-1,3-glucanase-III. Their optimum pH and temperature were 5 and 50 oC, respectively. |
| first_indexed | 2024-12-21T13:14:47Z |
| format | Article |
| id | doaj.art-2b7d044f6b464353be46e6d19c6a7ea4 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-21T13:14:47Z |
| publishDate | 2009-09-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-2b7d044f6b464353be46e6d19c6a7ea42022-12-21T19:02:46ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942009-09-01163115119Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reeseiSRI WAHYUNI BUDIARTISITI MUSLIMAH WIDYASTUTISEBASTIAN MARGINOTrichoderma enzymes that inhibit fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal root rot pathogen Ganoderma philippii. This experiment was aimed to purify and characterize the β-1,3-glucanase of T. reesei. Extracellular β-1,3-glucanase was produced by growing mycoparasite T. reesei isolate T13 in colloidal chitin and sucrose as carbon sources. The enzyme was then purified to its homogeneity by precipitation with ammonium sulfate, followed by gel filtration chromatography and chromatofocusing. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 12% was used to confirm the purity of enzyme at each stage of preparation and to characterize purified protein. The results showed that T. reesei produced at least three extracellular β-1,3-glucanases. Estimation of molecular weight based on SDS-PAGE 12% have three isoform of β-1,3-glucanase were 90 kDa for β-1,3-glucanase-I, 75 kDa for β-1,3-glucanase-II, and 64 kDa for β-1,3-glucanase-III. Their optimum pH and temperature were 5 and 50 oC, respectively.http://journal.ipb.ac.id/index.php/hayati/article/viewFile/282/147β-13-glucanaseTrichoderma reeseiGanoderma philippii |
| spellingShingle | SRI WAHYUNI BUDIARTI SITI MUSLIMAH WIDYASTUTI SEBASTIAN MARGINO Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei Hayati Journal of Biosciences β-1 3-glucanase Trichoderma reesei Ganoderma philippii |
| title | Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei |
| title_full | Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei |
| title_fullStr | Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei |
| title_full_unstemmed | Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei |
| title_short | Purification and Characterization of β-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei |
| title_sort | purification and characterization of β 1 3 glucanase from the antagonistic fungus trichoderma reesei |
| topic | β-1 3-glucanase Trichoderma reesei Ganoderma philippii |
| url | http://journal.ipb.ac.id/index.php/hayati/article/viewFile/282/147 |
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