Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis
Chemerin (CHEM) is a hormone mainly expressed in adipocytes involved in the regulation of energy homeostasis and inflammatory response. CHEM expression has been demonstrated in the structures of the porcine hypothalamic-pituitary-gonadal axis, as well as in the uterus, trophoblasts and conceptuses o...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/7/1161 |
| _version_ | 1797439897553862656 |
|---|---|
| author | Kinga Orzechowska Kamil Dobrzyń Marta Kieżun Agata Malinowska Bianka Świderska Tadeusz Kamiński Nina Smolińska |
| author_facet | Kinga Orzechowska Kamil Dobrzyń Marta Kieżun Agata Malinowska Bianka Świderska Tadeusz Kamiński Nina Smolińska |
| author_sort | Kinga Orzechowska |
| collection | DOAJ |
| description | Chemerin (CHEM) is a hormone mainly expressed in adipocytes involved in the regulation of energy homeostasis and inflammatory response. CHEM expression has been demonstrated in the structures of the porcine hypothalamic-pituitary-gonadal axis, as well as in the uterus, trophoblasts and conceptuses of pigs. In this study, we performed high-throughput proteomic analyses (liquid chromatography with tandem mass spectrometry, LC-MS/MS) to examine the influence of CHEM (400 ng/mL) on differentially regulated proteins (DRPs) in the porcine endometrial tissue explants during implantation (15 to 16 days of gestation). Among all 352 DRPs, 164 were up-regulated and 188 were down-regulated in CHEM-treated group. DRPs were assigned to 47 gene ontology (GO) terms (<i>p</i>-adjusted < 0.05). Validation of four DRPs (IFIT5, TGFβ1, ACO1 and PGRMC1) by Western blot analysis confirmed the veracity and accuracy of the LC-MS/MS method used in the present study. We suggest that CHEM, by modulating various protein expressions, takes part in the endometrial cell proliferation, migration and invasion at the time of implantation. It also regulates the endometrial immune response, sensitivity to P4 and the formation of new blood vessels. Additionally, CHEM appears to be an important factor involved in endothelial cell dysfunction during the pathogenesis of preeclampsia. The identification of a large number of DRPs under the influence of CHEM provides a valuable resource for understanding the molecular mechanisms of this hormone action during implantation, which is a prerequisite for better control of pig reproduction. |
| first_indexed | 2024-03-09T12:00:22Z |
| format | Article |
| id | doaj.art-2b7f84bca8144b48ba0be370deb738dc |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T12:00:22Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2b7f84bca8144b48ba0be370deb738dc2023-11-30T23:04:19ZengMDPI AGCells2073-44092022-03-01117116110.3390/cells11071161Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS AnalysisKinga Orzechowska0Kamil Dobrzyń1Marta Kieżun2Agata Malinowska3Bianka Świderska4Tadeusz Kamiński5Nina Smolińska6Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719 Olsztyn, PolandDepartment of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 5, 10-718 Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719 Olsztyn, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics PAS, 5a Pawińskiego St., 02-106 Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics PAS, 5a Pawińskiego St., 02-106 Warsaw, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719 Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719 Olsztyn, PolandChemerin (CHEM) is a hormone mainly expressed in adipocytes involved in the regulation of energy homeostasis and inflammatory response. CHEM expression has been demonstrated in the structures of the porcine hypothalamic-pituitary-gonadal axis, as well as in the uterus, trophoblasts and conceptuses of pigs. In this study, we performed high-throughput proteomic analyses (liquid chromatography with tandem mass spectrometry, LC-MS/MS) to examine the influence of CHEM (400 ng/mL) on differentially regulated proteins (DRPs) in the porcine endometrial tissue explants during implantation (15 to 16 days of gestation). Among all 352 DRPs, 164 were up-regulated and 188 were down-regulated in CHEM-treated group. DRPs were assigned to 47 gene ontology (GO) terms (<i>p</i>-adjusted < 0.05). Validation of four DRPs (IFIT5, TGFβ1, ACO1 and PGRMC1) by Western blot analysis confirmed the veracity and accuracy of the LC-MS/MS method used in the present study. We suggest that CHEM, by modulating various protein expressions, takes part in the endometrial cell proliferation, migration and invasion at the time of implantation. It also regulates the endometrial immune response, sensitivity to P4 and the formation of new blood vessels. Additionally, CHEM appears to be an important factor involved in endothelial cell dysfunction during the pathogenesis of preeclampsia. The identification of a large number of DRPs under the influence of CHEM provides a valuable resource for understanding the molecular mechanisms of this hormone action during implantation, which is a prerequisite for better control of pig reproduction.https://www.mdpi.com/2073-4409/11/7/1161chemerinpigimplantationuterusproteomeearly pregnancy |
| spellingShingle | Kinga Orzechowska Kamil Dobrzyń Marta Kieżun Agata Malinowska Bianka Świderska Tadeusz Kamiński Nina Smolińska Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis Cells chemerin pig implantation uterus proteome early pregnancy |
| title | Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis |
| title_full | Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis |
| title_fullStr | Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis |
| title_full_unstemmed | Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis |
| title_short | Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis |
| title_sort | chemerin effect on the endometrial proteome of the domestic pig during implantation obtained by lc ms ms analysis |
| topic | chemerin pig implantation uterus proteome early pregnancy |
| url | https://www.mdpi.com/2073-4409/11/7/1161 |
| work_keys_str_mv | AT kingaorzechowska chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT kamildobrzyn chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT martakiezun chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT agatamalinowska chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT biankaswiderska chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT tadeuszkaminski chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis AT ninasmolinska chemerineffectontheendometrialproteomeofthedomesticpigduringimplantationobtainedbylcmsmsanalysis |