Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis.
Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natura...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4530946?pdf=render |
| _version_ | 1817980874462855168 |
|---|---|
| author | G Hodge J W Upham A B Chang K J Baines S T Yerkovich S J Pizzutto S Hodge |
| author_facet | G Hodge J W Upham A B Chang K J Baines S T Yerkovich S J Pizzutto S Hodge |
| author_sort | G Hodge |
| collection | DOAJ |
| description | Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natural killer (NK) cells and NKT-like cells. We have shown that these cells produce increased cytotoxic (granzyme b and perforin) and inflammatory (IFNγ and TNFα) mediators in several adult chronic lung diseases and hypothesised that similar changes would be evident in children with BE.Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities. |
| first_indexed | 2024-04-13T22:58:28Z |
| format | Article |
| id | doaj.art-2b80b07bb300496493d253daa8a724e8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T22:58:28Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2b80b07bb300496493d253daa8a724e82022-12-22T02:25:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013369510.1371/journal.pone.0133695Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis.G HodgeJ W UphamA B ChangK J BainesS T YerkovichS J PizzuttoS HodgeBronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natural killer (NK) cells and NKT-like cells. We have shown that these cells produce increased cytotoxic (granzyme b and perforin) and inflammatory (IFNγ and TNFα) mediators in several adult chronic lung diseases and hypothesised that similar changes would be evident in children with BE.Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities.http://europepmc.org/articles/PMC4530946?pdf=render |
| spellingShingle | G Hodge J W Upham A B Chang K J Baines S T Yerkovich S J Pizzutto S Hodge Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. PLoS ONE |
| title | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. |
| title_full | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. |
| title_fullStr | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. |
| title_full_unstemmed | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. |
| title_short | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis. |
| title_sort | increased peripheral blood pro inflammatory cytotoxic lymphocytes in children with bronchiectasis |
| url | http://europepmc.org/articles/PMC4530946?pdf=render |
| work_keys_str_mv | AT ghodge increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT jwupham increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT abchang increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT kjbaines increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT styerkovich increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT sjpizzutto increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis AT shodge increasedperipheralbloodproinflammatorycytotoxiclymphocytesinchildrenwithbronchiectasis |