Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial
Background: Accumulating evidences suggest that date palm pollen (DPP) induces antioxidant activity and improvessemen parameters in male rats. However, there is a few scientific evidences in support of the DPP effects on humanmale fertility. Hence, the effect of oral consumption of DPP on sperm para...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-04-01
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| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | https://www.ijfs.ir/article_697936_f148664a2d0a814259eeab1087c27ab1.pdf |
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| author | Ali Mohammad Falahati Soghra Fallahi Zeinab Allamehzadeh Maliheh Izadi Raieni Kianoosh Malekzadeh |
| author_facet | Ali Mohammad Falahati Soghra Fallahi Zeinab Allamehzadeh Maliheh Izadi Raieni Kianoosh Malekzadeh |
| author_sort | Ali Mohammad Falahati |
| collection | DOAJ |
| description | Background: Accumulating evidences suggest that date palm pollen (DPP) induces antioxidant activity and improvessemen parameters in male rats. However, there is a few scientific evidences in support of the DPP effects on humanmale fertility. Hence, the effect of oral consumption of DPP on sperm parameters and expression pattern of Peroxiredoxin-1 (PRDX1) and Peroxiredoxin-6 (PRDX6) genes was evaluated in men with infertility.Materials and Methods: The current controlled clinical trial included 40 men with infertility (DPP group) and 10 normospermicfertile men as controls. The DPP group received gelatinous capsules of DPP (400 mg/kg) for 74 days. Semensampling was done before and after treatment in the both groups. Semen analysis and 8-isoprostane concentration assessmentswere performed by computer-assisted sperm analysis and ELISA methods, respectively. Quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) assays were employed to explore expression of PRDX1 and PRDX6 genes.Results: DPP consumption significantly improved semen volume (P=0.030), count (P<0.001) and morphology ofsperm (P=0.023). Concentration of 8-isoprostane was significantly decreased after intervention in the DPP group(P<0.001). DPP consumption led to a significant elevation in the expression of PRDX1 and PRDX6 genes (P<0.001).Elevated gene expression of PRDX6 and PRDX1 was positively correlated with improved parameters of sperm includingcount, volume, motility and morphology.Conclusion: Taken together, DPP seems to promote sperm quality through a decrease in reactive oxygen species(ROS) by increasing expression of antioxidant genes. Further large-scale studies are required to challenge this hypothesis(registration number: IRCT2015021221014N2) |
| first_indexed | 2024-04-09T12:36:12Z |
| format | Article |
| id | doaj.art-2b827b802c4041da8992b066ad60bf94 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-04-09T12:36:12Z |
| publishDate | 2023-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-2b827b802c4041da8992b066ad60bf942023-05-15T10:41:33ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782023-04-0117320120710.22074/ijfs.2022.549291.1264697936Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical TrialAli Mohammad Falahati0Soghra Fallahi1Zeinab Allamehzadeh2Maliheh Izadi Raieni3Kianoosh Malekzadeh4Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranHormozgan Institute of Health, Hormozgan University of Medical Sciences, Bandar Abbas, IranMedical Genetics and Prenatal Diagnostic Lab, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, IranMedical Genetics and Prenatal Diagnostic Lab, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, IranDepartment of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranBackground: Accumulating evidences suggest that date palm pollen (DPP) induces antioxidant activity and improvessemen parameters in male rats. However, there is a few scientific evidences in support of the DPP effects on humanmale fertility. Hence, the effect of oral consumption of DPP on sperm parameters and expression pattern of Peroxiredoxin-1 (PRDX1) and Peroxiredoxin-6 (PRDX6) genes was evaluated in men with infertility.Materials and Methods: The current controlled clinical trial included 40 men with infertility (DPP group) and 10 normospermicfertile men as controls. The DPP group received gelatinous capsules of DPP (400 mg/kg) for 74 days. Semensampling was done before and after treatment in the both groups. Semen analysis and 8-isoprostane concentration assessmentswere performed by computer-assisted sperm analysis and ELISA methods, respectively. Quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) assays were employed to explore expression of PRDX1 and PRDX6 genes.Results: DPP consumption significantly improved semen volume (P=0.030), count (P<0.001) and morphology ofsperm (P=0.023). Concentration of 8-isoprostane was significantly decreased after intervention in the DPP group(P<0.001). DPP consumption led to a significant elevation in the expression of PRDX1 and PRDX6 genes (P<0.001).Elevated gene expression of PRDX6 and PRDX1 was positively correlated with improved parameters of sperm includingcount, volume, motility and morphology.Conclusion: Taken together, DPP seems to promote sperm quality through a decrease in reactive oxygen species(ROS) by increasing expression of antioxidant genes. Further large-scale studies are required to challenge this hypothesis(registration number: IRCT2015021221014N2)https://www.ijfs.ir/article_697936_f148664a2d0a814259eeab1087c27ab1.pdf8-isoprostanemale infertilityprdx1prdx6 |
| spellingShingle | Ali Mohammad Falahati Soghra Fallahi Zeinab Allamehzadeh Maliheh Izadi Raieni Kianoosh Malekzadeh Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial International Journal of Fertility and Sterility 8-isoprostane male infertility prdx1 prdx6 |
| title | Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial |
| title_full | Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial |
| title_fullStr | Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial |
| title_full_unstemmed | Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial |
| title_short | Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial |
| title_sort | effects of date palm pollen supplementations on the expression of prdx1 and prdx6 genes in infertile men a controlled clinical trial |
| topic | 8-isoprostane male infertility prdx1 prdx6 |
| url | https://www.ijfs.ir/article_697936_f148664a2d0a814259eeab1087c27ab1.pdf |
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