Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)
Elongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 g...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1270776/full |
| _version_ | 1797676734844239872 |
|---|---|
| author | Wan-Hong Zeng Wan-Hong Zeng Xiu-Ying Wei Wei Qin Chuan-Jie Qin Qiong Shi Qiong Shi Qiong Shi Sheng-Tao Guo Panita Prathomya Shi-Yong Zhang Peng Fu Wei Hu Han-Wen Yuan Zheng-Yong Wen Zheng-Yong Wen Zheng-Yong Wen |
| author_facet | Wan-Hong Zeng Wan-Hong Zeng Xiu-Ying Wei Wei Qin Chuan-Jie Qin Qiong Shi Qiong Shi Qiong Shi Sheng-Tao Guo Panita Prathomya Shi-Yong Zhang Peng Fu Wei Hu Han-Wen Yuan Zheng-Yong Wen Zheng-Yong Wen Zheng-Yong Wen |
| author_sort | Wan-Hong Zeng |
| collection | DOAJ |
| description | Elongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 gene was identified and characterized from yellow catfish Pelteobagrus fulvidraco, and then its evolutionary and molecular characteristics as well as transcriptional changes in response to various nutritional status were determined. Results showed that the open reading frame (ORF) of elovl8 was 795 bp in length, encoding a protein of 264 amino acids. Multiple sequences alignment showed that the yellow catfish Elovl8 was highly conserved with other homologs in teleosts, sharing similar structural characteristics (including six conserved transmembrane α-helical domains, four conserved elongase motifs, and three highly conserved cysteine residues). Meanwhile, comparisons of genetic synteny confirmed that the elovl8 gene identified from the yellow catfish was the homolog of elovl8b in other teleosts, and thus, the elovl8a gene was lost in the genome of the yellow catfish. Gene structure analysis revealed that the elovl8b gene contained eight exons and seven introns, which was highly conserved in teleosts, implying the functional conservation among various fish species. Tissue distribution analysis detected by real-time quantitative PCR (RT-qPCR) showed that the elovl8 gene was extensively expressed in all detected tissues except eyes, with high expression levels in the intestine and liver. Temporal expression analysis revealed that the expression level of elovl8 was stably expressed in the early 12 h after fertilization, and then dramatically decreased at 24, 48, 72, and 96 h after fertilization, implying that elovl8 is required for HUFA biosynthesis in the early development stages. Functional experiments showed that the expression of the elovl8 gene was stimulated after feeding with egg yolk but was not obviously affected after feeding with halogenated worms, indicating that diets full of HUFAs can inhibit the expression of elovl8 in yellow catfish. Our findings will help us to better understand the evolutionary and functional characteristics of elovl8 in teleosts, and lay a solid basis for investigating the regulation mechanism of HUFA biosynthesis. |
| first_indexed | 2024-03-11T22:34:37Z |
| format | Article |
| id | doaj.art-2b833a37d8b046d8ad886bf9607871ff |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-11T22:34:37Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-2b833a37d8b046d8ad886bf9607871ff2023-09-22T15:32:57ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-09-011010.3389/fmars.2023.12707761270776Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)Wan-Hong Zeng0Wan-Hong Zeng1Xiu-Ying Wei2Wei Qin3Chuan-Jie Qin4Qiong Shi5Qiong Shi6Qiong Shi7Sheng-Tao Guo8Panita Prathomya9Shi-Yong Zhang10Peng Fu11Wei Hu12Han-Wen Yuan13Zheng-Yong Wen14Zheng-Yong Wen15Zheng-Yong Wen16School of Animal Science, Yangtze University, Jingzhou, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, ChinaLaboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaKey Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, ChinaDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandFreshwater Fisheries Research Institute of Jiangsu Province, Nanjing, ChinaChongqing Fisheries Science Research Institute, Chongqing, ChinaSchool of Animal Science, Yangtze University, Jingzhou, ChinaSchool of Animal Science, Yangtze University, Jingzhou, ChinaSchool of Animal Science, Yangtze University, Jingzhou, ChinaKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, ChinaElongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 gene was identified and characterized from yellow catfish Pelteobagrus fulvidraco, and then its evolutionary and molecular characteristics as well as transcriptional changes in response to various nutritional status were determined. Results showed that the open reading frame (ORF) of elovl8 was 795 bp in length, encoding a protein of 264 amino acids. Multiple sequences alignment showed that the yellow catfish Elovl8 was highly conserved with other homologs in teleosts, sharing similar structural characteristics (including six conserved transmembrane α-helical domains, four conserved elongase motifs, and three highly conserved cysteine residues). Meanwhile, comparisons of genetic synteny confirmed that the elovl8 gene identified from the yellow catfish was the homolog of elovl8b in other teleosts, and thus, the elovl8a gene was lost in the genome of the yellow catfish. Gene structure analysis revealed that the elovl8b gene contained eight exons and seven introns, which was highly conserved in teleosts, implying the functional conservation among various fish species. Tissue distribution analysis detected by real-time quantitative PCR (RT-qPCR) showed that the elovl8 gene was extensively expressed in all detected tissues except eyes, with high expression levels in the intestine and liver. Temporal expression analysis revealed that the expression level of elovl8 was stably expressed in the early 12 h after fertilization, and then dramatically decreased at 24, 48, 72, and 96 h after fertilization, implying that elovl8 is required for HUFA biosynthesis in the early development stages. Functional experiments showed that the expression of the elovl8 gene was stimulated after feeding with egg yolk but was not obviously affected after feeding with halogenated worms, indicating that diets full of HUFAs can inhibit the expression of elovl8 in yellow catfish. Our findings will help us to better understand the evolutionary and functional characteristics of elovl8 in teleosts, and lay a solid basis for investigating the regulation mechanism of HUFA biosynthesis.https://www.frontiersin.org/articles/10.3389/fmars.2023.1270776/fullyellow catfishelovl8gene cloninggene expressionHUFA biosynthesis |
| spellingShingle | Wan-Hong Zeng Wan-Hong Zeng Xiu-Ying Wei Wei Qin Chuan-Jie Qin Qiong Shi Qiong Shi Qiong Shi Sheng-Tao Guo Panita Prathomya Shi-Yong Zhang Peng Fu Wei Hu Han-Wen Yuan Zheng-Yong Wen Zheng-Yong Wen Zheng-Yong Wen Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) Frontiers in Marine Science yellow catfish elovl8 gene cloning gene expression HUFA biosynthesis |
| title | Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) |
| title_full | Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) |
| title_fullStr | Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) |
| title_full_unstemmed | Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) |
| title_short | Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco) |
| title_sort | molecular characterization spatiotemporal expression patterns of fatty acid elongase elovl8 gene and its transcription changes in response to different diet stimuli in yellow catfish pelteobagrus fulvidraco |
| topic | yellow catfish elovl8 gene cloning gene expression HUFA biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1270776/full |
| work_keys_str_mv | AT wanhongzeng molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT wanhongzeng molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT xiuyingwei molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT weiqin molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT chuanjieqin molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT qiongshi molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT qiongshi molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT qiongshi molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT shengtaoguo molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT panitaprathomya molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT shiyongzhang molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT pengfu molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT weihu molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT hanwenyuan molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT zhengyongwen molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT zhengyongwen molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco AT zhengyongwen molecularcharacterizationspatiotemporalexpressionpatternsoffattyacidelongaseelovl8geneanditstranscriptionchangesinresponsetodifferentdietstimuliinyellowcatfishpelteobagrusfulvidraco |