Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe
Abstract Lysosomes are multifunctional organelles involved in macromolecule degradation, nutrient sensing, and autophagy. Live imaging has revealed lysosome subpopulations with dynamics and characteristic cellular localization. An as‐yet unanswered question is whether lysosomes are spatially organiz...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Exploration |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/EXP.20210215 |
| _version_ | 1811333438569971712 |
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| author | Lei Wang Rui Chen Guanqun Han Xuan Liu Taosheng Huang Jiajie Diao Yujie Sun |
| author_facet | Lei Wang Rui Chen Guanqun Han Xuan Liu Taosheng Huang Jiajie Diao Yujie Sun |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Abstract Lysosomes are multifunctional organelles involved in macromolecule degradation, nutrient sensing, and autophagy. Live imaging has revealed lysosome subpopulations with dynamics and characteristic cellular localization. An as‐yet unanswered question is whether lysosomes are spatially organized to coordinate and integrate their functions. Combined with super‐resolution microscopy, we designed a small organic fluorescent probe—TPAE—that targeted lysosomes with a large Stokes shift. When we analyzed the spatial organization of lysosomes against mitochondria in different cell lines with this probe, we discovered different distance distribution patterns between lysosomes and mitochondria during increased autophagy flux. By using SLC25A46 mutation fibroblasts derived from patients containing highly fused mitochondria with low oxidative phosphorylation, we concluded that unhealthy mitochondria redistributed the subcellular localization of lysosomes, which implies a strong connection between mitochondria and lysosomes. |
| first_indexed | 2024-04-13T16:52:40Z |
| format | Article |
| id | doaj.art-2b836ed23d0f4a7b859ce06310553691 |
| institution | Directory Open Access Journal |
| issn | 2766-8509 2766-2098 |
| language | English |
| last_indexed | 2024-04-13T16:52:40Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj.art-2b836ed23d0f4a7b859ce063105536912022-12-22T02:38:54ZengWileyExploration2766-85092766-20982022-06-0123n/an/a10.1002/EXP.20210215Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probeLei Wang0Rui Chen1Guanqun Han2Xuan Liu3Taosheng Huang4Jiajie Diao5Yujie Sun6Department of Cancer Biology, College of Medicine University of Cincinnati Cincinnati Ohio USADepartment of Chemistry University of Cincinnati Cincinnati Ohio USADepartment of Chemistry University of Cincinnati Cincinnati Ohio USADepartment of Chemistry University of Cincinnati Cincinnati Ohio USADepartment of Pediatrics, Jacobs School of Medicine and Biomedical Sciences University at Buffalo Buffalo New York USADepartment of Cancer Biology, College of Medicine University of Cincinnati Cincinnati Ohio USADepartment of Chemistry University of Cincinnati Cincinnati Ohio USAAbstract Lysosomes are multifunctional organelles involved in macromolecule degradation, nutrient sensing, and autophagy. Live imaging has revealed lysosome subpopulations with dynamics and characteristic cellular localization. An as‐yet unanswered question is whether lysosomes are spatially organized to coordinate and integrate their functions. Combined with super‐resolution microscopy, we designed a small organic fluorescent probe—TPAE—that targeted lysosomes with a large Stokes shift. When we analyzed the spatial organization of lysosomes against mitochondria in different cell lines with this probe, we discovered different distance distribution patterns between lysosomes and mitochondria during increased autophagy flux. By using SLC25A46 mutation fibroblasts derived from patients containing highly fused mitochondria with low oxidative phosphorylation, we concluded that unhealthy mitochondria redistributed the subcellular localization of lysosomes, which implies a strong connection between mitochondria and lysosomes.https://doi.org/10.1002/EXP.20210215fibroblastslysosomesmitochondriaspatial organizationsuper resolution microscopy |
| spellingShingle | Lei Wang Rui Chen Guanqun Han Xuan Liu Taosheng Huang Jiajie Diao Yujie Sun Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe Exploration fibroblasts lysosomes mitochondria spatial organization super resolution microscopy |
| title | Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| title_full | Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| title_fullStr | Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| title_full_unstemmed | Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| title_short | Super‐resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| title_sort | super resolution analyzing spatial organization of lysosomes with an organic fluorescent probe |
| topic | fibroblasts lysosomes mitochondria spatial organization super resolution microscopy |
| url | https://doi.org/10.1002/EXP.20210215 |
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