Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease
Nerve Growth Factor (NGF) and Brain derived Neurotrophic Factor (BDNF) mature/precursor imbalance in tears and serum is suggested as a risk factor and symptomatology aggravation in ophthalmology and neuropsychiatric disturbances. Cognitive and mood alterations are reported by patients with Graves’ O...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8074 |
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| author | Alice Bruscolini Angela Iannitelli Marco Segatto Pamela Rosso Elena Fico Marzia Buonfiglio Alessandro Lambiase Paola Tirassa |
| author_facet | Alice Bruscolini Angela Iannitelli Marco Segatto Pamela Rosso Elena Fico Marzia Buonfiglio Alessandro Lambiase Paola Tirassa |
| author_sort | Alice Bruscolini |
| collection | DOAJ |
| description | Nerve Growth Factor (NGF) and Brain derived Neurotrophic Factor (BDNF) mature/precursor imbalance in tears and serum is suggested as a risk factor and symptomatology aggravation in ophthalmology and neuropsychiatric disturbances. Cognitive and mood alterations are reported by patients with Graves’ Orbitopathy (GO), indicating neurotrophin alterations might be involved. To address this question, the expression levels of NGF and BDNF and their precursors in serum and tears of GO patients were analyzed and correlated with the ophthalmological and psycho-cognitive symptoms. Hamilton Rating Scale for Anxiety (HAM-A) and Depression (HAM-D), Temperament and Character Inventory (TCI), and Cambridge Neuropsychological Test Automated Battery (CANTAB) test were used as a score. NGF and BDNF levels were measured using ELISA and Western Blot and statistically analyzed for psychiatric/ocular variable trend association. GO patients show memorization time and level of distraction increase, together with high irritability and impulsiveness. HAM-A and CANTAB variables association, and some TCI dimensions are also found. NGF and BDNF expression correlates with ophthalmological symptoms only in tears, while mature/precursor NGF and BDNF correlate with the specific psycho-cognitive variables both in tears and serum. Our study is the first to show that changes in NGF and BDNF processing in tears and serum might profile ocular and cognitive alterations in patients. |
| first_indexed | 2024-03-11T04:16:55Z |
| format | Article |
| id | doaj.art-2b83d2ed91a54ae09578b60240c0bbbb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:55Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2b83d2ed91a54ae09578b60240c0bbbb2023-11-17T23:04:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01249807410.3390/ijms24098074Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ DiseaseAlice Bruscolini0Angela Iannitelli1Marco Segatto2Pamela Rosso3Elena Fico4Marzia Buonfiglio5Alessandro Lambiase6Paola Tirassa7Department of Sense Organs, University Sapienza of Rome, Viale del Policlinico 155, 00161 Rome, ItalyDepartment of Biotechnological and Applied Clinical Sciences, University of L’Aquila, Via Vetoio, Coppito 2, 67100 L’Aquila, ItalyDepartment of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche, ItalyInstitute of Biochemistry & Cell Biology (IBBC), National Research Council (CNR), Unit of Translational & Biomolecular Medicine “Rita Levi-Montalcini”, Viale dell’Università 33, 00185 Rome, ItalyInstitute of Biochemistry & Cell Biology (IBBC), National Research Council (CNR), Unit of Translational & Biomolecular Medicine “Rita Levi-Montalcini”, Viale dell’Università 33, 00185 Rome, ItalyHeadache Center, Policlinico Umberto I, Sapienza University, Viale dell’Università 33, 00185 Rome, ItalyDepartment of Sense Organs, University Sapienza of Rome, Viale del Policlinico 155, 00161 Rome, ItalyInstitute of Biochemistry & Cell Biology (IBBC), National Research Council (CNR), Unit of Translational & Biomolecular Medicine “Rita Levi-Montalcini”, Viale dell’Università 33, 00185 Rome, ItalyNerve Growth Factor (NGF) and Brain derived Neurotrophic Factor (BDNF) mature/precursor imbalance in tears and serum is suggested as a risk factor and symptomatology aggravation in ophthalmology and neuropsychiatric disturbances. Cognitive and mood alterations are reported by patients with Graves’ Orbitopathy (GO), indicating neurotrophin alterations might be involved. To address this question, the expression levels of NGF and BDNF and their precursors in serum and tears of GO patients were analyzed and correlated with the ophthalmological and psycho-cognitive symptoms. Hamilton Rating Scale for Anxiety (HAM-A) and Depression (HAM-D), Temperament and Character Inventory (TCI), and Cambridge Neuropsychological Test Automated Battery (CANTAB) test were used as a score. NGF and BDNF levels were measured using ELISA and Western Blot and statistically analyzed for psychiatric/ocular variable trend association. GO patients show memorization time and level of distraction increase, together with high irritability and impulsiveness. HAM-A and CANTAB variables association, and some TCI dimensions are also found. NGF and BDNF expression correlates with ophthalmological symptoms only in tears, while mature/precursor NGF and BDNF correlate with the specific psycho-cognitive variables both in tears and serum. Our study is the first to show that changes in NGF and BDNF processing in tears and serum might profile ocular and cognitive alterations in patients.https://www.mdpi.com/1422-0067/24/9/8074NGFBDNFGraves’ orbitopathyCANTAB testcognitive impairmentneuropsychiatric disorders |
| spellingShingle | Alice Bruscolini Angela Iannitelli Marco Segatto Pamela Rosso Elena Fico Marzia Buonfiglio Alessandro Lambiase Paola Tirassa Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease International Journal of Molecular Sciences NGF BDNF Graves’ orbitopathy CANTAB test cognitive impairment neuropsychiatric disorders |
| title | Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease |
| title_full | Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease |
| title_fullStr | Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease |
| title_full_unstemmed | Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease |
| title_short | Psycho-Cognitive Profile and NGF and BDNF Levels in Tears and Serum: A Pilot Study in Patients with Graves’ Disease |
| title_sort | psycho cognitive profile and ngf and bdnf levels in tears and serum a pilot study in patients with graves disease |
| topic | NGF BDNF Graves’ orbitopathy CANTAB test cognitive impairment neuropsychiatric disorders |
| url | https://www.mdpi.com/1422-0067/24/9/8074 |
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