Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines
In head and neck cancers, the effectiveness of cisplatin (CisPt) treatment is limited by its toxicity, especially when higher doses are necessary, and the possible occurrence of cisplatin resistance. This study evaluated the effects of resveratrol (RSV) on the expression of different genes involved...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6322 |
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| author | Marinela Bostan Mirela Mihaila Georgiana Gabriela Petrica-Matei Nicoleta Radu Razvan Hainarosie Cristian Dragos Stefanescu Viviana Roman Carmen Cristina Diaconu |
| author_facet | Marinela Bostan Mirela Mihaila Georgiana Gabriela Petrica-Matei Nicoleta Radu Razvan Hainarosie Cristian Dragos Stefanescu Viviana Roman Carmen Cristina Diaconu |
| author_sort | Marinela Bostan |
| collection | DOAJ |
| description | In head and neck cancers, the effectiveness of cisplatin (CisPt) treatment is limited by its toxicity, especially when higher doses are necessary, and the possible occurrence of cisplatin resistance. This study evaluated the effects of resveratrol (RSV) on the expression of different genes involved in the response of human tumor cells (FaDu, PE/CA-PJ49) to cisplatin therapy. Our results revealed that RSV induced apoptosis amplification in both FaDu and PE/CA-PJ49 cells and modulated the expression of specific genes differently than in normal HaCaT cells. In FaDu cells, combined CisPt + RSV treatment induced an increase in apoptosis, which was associated with an increase in <i>c-MYC</i> and <i>TP53</i> and a decrease in <i>BCL-2</i> expression. While CisPt + RSV treatment induced apoptosis in PE/CA-PJ49 cells by inhibition of <i>BCL-2</i> associated with high levels of <i>MDM-2</i> and subsequently led to inhibition of <i>TP53</i> gene expression. Decreased <i>c-MYC</i> expression in PE/CA-PJ49 treated with CisPt + RSV was accompanied by cell cycle blockage in G0/G1 phase. In conclusion, RSV influences tumor cell response to CisPt by inducing apoptosis and modulating gene expression. In addition, in normal HaCaT cells, RSV was able to reduce the harmful effects of CisPt. |
| first_indexed | 2024-03-10T10:27:05Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:27:05Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2b8461f8b7f140f595cfda02608900d92023-11-21T23:55:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212632210.3390/ijms22126322Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell LinesMarinela Bostan0Mirela Mihaila1Georgiana Gabriela Petrica-Matei2Nicoleta Radu3Razvan Hainarosie4Cristian Dragos Stefanescu5Viviana Roman6Carmen Cristina Diaconu7Center of Immunology, Stefan S. Nicolau Institute of Virology, 030304 Bucharest, RomaniaCenter of Immunology, Stefan S. Nicolau Institute of Virology, 030304 Bucharest, RomaniaPersonal Genetics-Medical Genetics Center, Department of Cytogenetics, 010987 Bucharest, RomaniaDepartment of Biotechnology, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, RomaniaOtorhinolaryngology and Head and Neck Surgery Department—Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, RomaniaOtorhinolaryngology and Head and Neck Surgery Department—Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, RomaniaCenter of Immunology, Stefan S. Nicolau Institute of Virology, 030304 Bucharest, RomaniaDepartment of Cellular and Molecular Pathology, Stefan S. Nicolau Institute of Virology, 030304 Bucharest, RomaniaIn head and neck cancers, the effectiveness of cisplatin (CisPt) treatment is limited by its toxicity, especially when higher doses are necessary, and the possible occurrence of cisplatin resistance. This study evaluated the effects of resveratrol (RSV) on the expression of different genes involved in the response of human tumor cells (FaDu, PE/CA-PJ49) to cisplatin therapy. Our results revealed that RSV induced apoptosis amplification in both FaDu and PE/CA-PJ49 cells and modulated the expression of specific genes differently than in normal HaCaT cells. In FaDu cells, combined CisPt + RSV treatment induced an increase in apoptosis, which was associated with an increase in <i>c-MYC</i> and <i>TP53</i> and a decrease in <i>BCL-2</i> expression. While CisPt + RSV treatment induced apoptosis in PE/CA-PJ49 cells by inhibition of <i>BCL-2</i> associated with high levels of <i>MDM-2</i> and subsequently led to inhibition of <i>TP53</i> gene expression. Decreased <i>c-MYC</i> expression in PE/CA-PJ49 treated with CisPt + RSV was accompanied by cell cycle blockage in G0/G1 phase. In conclusion, RSV influences tumor cell response to CisPt by inducing apoptosis and modulating gene expression. In addition, in normal HaCaT cells, RSV was able to reduce the harmful effects of CisPt.https://www.mdpi.com/1422-0067/22/12/6322resveratrolcisplatinhead and neck carcinoma line<i>c-MYC</i><i>BCL-2</i><i>TP53</i> |
| spellingShingle | Marinela Bostan Mirela Mihaila Georgiana Gabriela Petrica-Matei Nicoleta Radu Razvan Hainarosie Cristian Dragos Stefanescu Viviana Roman Carmen Cristina Diaconu Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines International Journal of Molecular Sciences resveratrol cisplatin head and neck carcinoma line <i>c-MYC</i> <i>BCL-2</i> <i>TP53</i> |
| title | Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines |
| title_full | Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines |
| title_fullStr | Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines |
| title_full_unstemmed | Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines |
| title_short | Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines |
| title_sort | resveratrol modulation of apoptosis and cell cycle response to cisplatin in head and neck cancer cell lines |
| topic | resveratrol cisplatin head and neck carcinoma line <i>c-MYC</i> <i>BCL-2</i> <i>TP53</i> |
| url | https://www.mdpi.com/1422-0067/22/12/6322 |
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