Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2
IntroductionDengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent de...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1260812/full |
| _version_ | 1827818081776304128 |
|---|---|
| author | Devojit Kumar Sarma Lokendra Rathod Sweta Mishra Deepanker Das Ankita Agarwal Gaurav Sharma Tanim Arpit Singh Manoj Kumawat Samradhi Singh Vinod Verma Manoj Kumar Swasti Shubham Rajnarayan R. Tiwari Anil Prakash |
| author_facet | Devojit Kumar Sarma Lokendra Rathod Sweta Mishra Deepanker Das Ankita Agarwal Gaurav Sharma Tanim Arpit Singh Manoj Kumawat Samradhi Singh Vinod Verma Manoj Kumar Swasti Shubham Rajnarayan R. Tiwari Anil Prakash |
| author_sort | Devojit Kumar Sarma |
| collection | DOAJ |
| description | IntroductionDengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases.ObjectivesThe present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India.MethodsAedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis.ResultsDENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India.ConclusionThe observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential. |
| first_indexed | 2024-03-12T00:42:56Z |
| format | Article |
| id | doaj.art-2b84b6bd974a40b6b8d2e04f31b9ed5e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T00:42:56Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2b84b6bd974a40b6b8d2e04f31b9ed5e2023-09-15T01:24:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12608121260812Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2Devojit Kumar Sarma0Lokendra Rathod1Sweta Mishra2Deepanker Das3Ankita Agarwal4Gaurav Sharma5Tanim Arpit Singh6Manoj Kumawat7Samradhi Singh8Vinod Verma9Manoj Kumar10Swasti Shubham11Rajnarayan R. Tiwari12Anil Prakash13ICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaState Virology Laboratory, Department of Microbiology, Gandhi Medical College, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaMaharaja Ranjit Singh College of Professional Sciences, Indore, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaSanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaICMR-National Institute for Research in Environmental Health, Bhopal, IndiaIntroductionDengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases.ObjectivesThe present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India.MethodsAedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis.ResultsDENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India.ConclusionThe observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1260812/fulldengue virusAedes mosquitosurveillanceepidemiologyIndia font: Italiccomplex script font: Italic considering the growing threat |
| spellingShingle | Devojit Kumar Sarma Lokendra Rathod Sweta Mishra Deepanker Das Ankita Agarwal Gaurav Sharma Tanim Arpit Singh Manoj Kumawat Samradhi Singh Vinod Verma Manoj Kumar Swasti Shubham Rajnarayan R. Tiwari Anil Prakash Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 Frontiers in Microbiology dengue virus Aedes mosquito surveillance epidemiology India font: Italic complex script font: Italic considering the growing threat |
| title | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
| title_full | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
| title_fullStr | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
| title_full_unstemmed | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
| title_short | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
| title_sort | molecular surveillance of dengue virus in field collected aedes mosquitoes from bhopal central india evidence of circulation of a new lineage of serotype 2 |
| topic | dengue virus Aedes mosquito surveillance epidemiology India font: Italic complex script font: Italic considering the growing threat |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1260812/full |
| work_keys_str_mv | AT devojitkumarsarma molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT lokendrarathod molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT swetamishra molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT deepankerdas molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT ankitaagarwal molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT gauravsharma molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT tanimarpitsingh molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT manojkumawat molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT samradhisingh molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT vinodverma molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT manojkumar molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT swastishubham molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT rajnarayanrtiwari molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT anilprakash molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 |