Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber
There is an increasing demand for transdermal transport measurements to optimize topical drug formulations and to achieve proper penetration profile of cosmetic ingredients. Reflecting ethical concerns the use of both human and animal tissues is becoming more restricted. Therefore, the focus of derm...
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MDPI AG
2021-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/6/910 |
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| author | Júlia Tárnoki-Zách Elod Mehes Zsófia Varga-Medveczky Dona Greta Isai Nandor Barany Edina Bugyik Zsolt Revesz Sándor Paku Franciska Erdo Andras Czirok |
| author_facet | Júlia Tárnoki-Zách Elod Mehes Zsófia Varga-Medveczky Dona Greta Isai Nandor Barany Edina Bugyik Zsolt Revesz Sándor Paku Franciska Erdo Andras Czirok |
| author_sort | Júlia Tárnoki-Zách |
| collection | DOAJ |
| description | There is an increasing demand for transdermal transport measurements to optimize topical drug formulations and to achieve proper penetration profile of cosmetic ingredients. Reflecting ethical concerns the use of both human and animal tissues is becoming more restricted. Therefore, the focus of dermal research is shifting towards in vitro assays. In the current proof-of-concept study a three-layer skin equivalent using human HaCaT keratinocytes, an electrospun polycaprolactone mesh and a collagen-I gel was compared to human excised skin samples. We measured the permeability of the samples for 2% caffeine cream using a miniaturized dynamic diffusion cell (“skin-on-a-chip” microfluidic device). Caffeine delivery exhibits similar transport kinetics through the artificial skin and the human tissue: after a rapid rise, a long-lasting high concentration steady state develops. This is markedly distinct from the kinetics measured when using cell-free constructs, where a shorter release was observable. These results imply that both the established skin equivalent and the microfluidic diffusion chamber can serve as a suitable base for further development of more complex tissue substitutes. |
| first_indexed | 2024-03-10T10:14:26Z |
| format | Article |
| id | doaj.art-2b87c923bc8d49c8b74449f7fb400ee3 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T10:14:26Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2b87c923bc8d49c8b74449f7fb400ee32023-11-22T00:54:44ZengMDPI AGPharmaceutics1999-49232021-06-0113691010.3390/pharmaceutics13060910Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion ChamberJúlia Tárnoki-Zách0Elod Mehes1Zsófia Varga-Medveczky2Dona Greta Isai3Nandor Barany4Edina Bugyik5Zsolt Revesz6Sándor Paku7Franciska Erdo8Andras Czirok9Department of Biological Physics, Eotvos University, 1117 Budapest, HungaryDepartment of Biological Physics, Eotvos University, 1117 Budapest, HungaryFaculty of Information Technology and Bionics, Pázmány Péter Catholic University, 1083 Budapest, HungaryDepartment of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USAFirst Department of Pathology and Experimental Cancer Research, Semmelweis University, 1085 Budapest, HungaryFirst Department of Pathology and Experimental Cancer Research, Semmelweis University, 1085 Budapest, HungaryRevesz Plasztika, 1125 Budapest, HungaryFirst Department of Pathology and Experimental Cancer Research, Semmelweis University, 1085 Budapest, HungaryFaculty of Information Technology and Bionics, Pázmány Péter Catholic University, 1083 Budapest, HungaryDepartment of Biological Physics, Eotvos University, 1117 Budapest, HungaryThere is an increasing demand for transdermal transport measurements to optimize topical drug formulations and to achieve proper penetration profile of cosmetic ingredients. Reflecting ethical concerns the use of both human and animal tissues is becoming more restricted. Therefore, the focus of dermal research is shifting towards in vitro assays. In the current proof-of-concept study a three-layer skin equivalent using human HaCaT keratinocytes, an electrospun polycaprolactone mesh and a collagen-I gel was compared to human excised skin samples. We measured the permeability of the samples for 2% caffeine cream using a miniaturized dynamic diffusion cell (“skin-on-a-chip” microfluidic device). Caffeine delivery exhibits similar transport kinetics through the artificial skin and the human tissue: after a rapid rise, a long-lasting high concentration steady state develops. This is markedly distinct from the kinetics measured when using cell-free constructs, where a shorter release was observable. These results imply that both the established skin equivalent and the microfluidic diffusion chamber can serve as a suitable base for further development of more complex tissue substitutes.https://www.mdpi.com/1999-4923/13/6/910skin equivalentelectrospun meshmicrofluidic diffusion chambertransepithelial transport kinetic3D printed device |
| spellingShingle | Júlia Tárnoki-Zách Elod Mehes Zsófia Varga-Medveczky Dona Greta Isai Nandor Barany Edina Bugyik Zsolt Revesz Sándor Paku Franciska Erdo Andras Czirok Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber Pharmaceutics skin equivalent electrospun mesh microfluidic diffusion chamber transepithelial transport kinetic 3D printed device |
| title | Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber |
| title_full | Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber |
| title_fullStr | Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber |
| title_full_unstemmed | Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber |
| title_short | Development and Evaluation of a Human Skin Equivalent in a Semiautomatic Microfluidic Diffusion Chamber |
| title_sort | development and evaluation of a human skin equivalent in a semiautomatic microfluidic diffusion chamber |
| topic | skin equivalent electrospun mesh microfluidic diffusion chamber transepithelial transport kinetic 3D printed device |
| url | https://www.mdpi.com/1999-4923/13/6/910 |
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