Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study
BackgroundRecent studies have shown that an imbalance in gut microbiota (GM) may not always be associated with endometriosis (EMS). To investigate this further, we conducted a two-sample Mendelian randomization study.MethodsMR analysis was performed on genome-wide association study (GWAS) summary st...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188458/full |
| _version_ | 1797671932985868288 |
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| author | Xuan Ji Qi Yang Xiu-Lin Zhu Li Xu Jie-Ying Guo Yan Rong Yun-Lang Cai |
| author_facet | Xuan Ji Qi Yang Xiu-Lin Zhu Li Xu Jie-Ying Guo Yan Rong Yun-Lang Cai |
| author_sort | Xuan Ji |
| collection | DOAJ |
| description | BackgroundRecent studies have shown that an imbalance in gut microbiota (GM) may not always be associated with endometriosis (EMS). To investigate this further, we conducted a two-sample Mendelian randomization study.MethodsMR analysis was performed on genome-wide association study (GWAS) summary statistics of GM and EMS. Specifically, the MiBioGen microbiota GWAS (N = 18,340) was used as exposure. The FinnGen study GWAS (8,288 EMS cases and 68,969 controls) was used as outcome. We primarily used the inverse variance weighted (IVW) method to analyze the correlation and conducted a sensitivity analysis to verify its reliability.Results(1) MR analysis: The results of the IVW method confirmed that a total of 8 GM taxa were related to the risk of EMS. Class-Melainabacteria (p = 0.036), family-Ruminococcaceae (p = 0.037), and genus-Eubacteriumruminantium (p = 0.015) had a protective effect on EMS, whereas order-Bacillales (p = 0.046), family-Prevotellaceae (p = 0.027), genus-Anaerotruncus (p = 0.025), genus-Olsenella (p = 0.036) and genus-RuminococcaceaeUCG002 (p = 0.035) could increase the risk of EMS. (2) Sensitivity analysis: Cochrane’s Q test (p > 0.05), MR-Egger intercept method (p > 0.05), and leave-one-out method confirmed the robustness of MR results.ConclusionThis study performed a MR analysis on two large national databases and identified the association between 8 GM taxa and EMS. These taxa could potentially be utilized for indirectly diagnosing EMS and could lead to novel perspectives in research regarding the pathogenesis, diagnosis, and treatment of EMS. |
| first_indexed | 2024-03-11T21:22:43Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T21:22:43Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-2b88125aa8c540718c08bb13e70203dd2023-09-28T05:28:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.11884581188458Association between gut microbiota and endometriosis: a two-sample Mendelian randomization studyXuan Ji0Qi Yang1Xiu-Lin Zhu2Li Xu3Jie-Ying Guo4Yan Rong5Yun-Lang Cai6Medical School of Southeast University, Nanjing, Jiangsu Province, ChinaMedical School of Southeast University, Nanjing, Jiangsu Province, ChinaDepartment of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai, ChinaMedical School of Southeast University, Nanjing, Jiangsu Province, ChinaMedical School of Southeast University, Nanjing, Jiangsu Province, ChinaMedical School of Southeast University, Nanjing, Jiangsu Province, ChinaDepartment of Obstetrics and Gynecology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu Province, ChinaBackgroundRecent studies have shown that an imbalance in gut microbiota (GM) may not always be associated with endometriosis (EMS). To investigate this further, we conducted a two-sample Mendelian randomization study.MethodsMR analysis was performed on genome-wide association study (GWAS) summary statistics of GM and EMS. Specifically, the MiBioGen microbiota GWAS (N = 18,340) was used as exposure. The FinnGen study GWAS (8,288 EMS cases and 68,969 controls) was used as outcome. We primarily used the inverse variance weighted (IVW) method to analyze the correlation and conducted a sensitivity analysis to verify its reliability.Results(1) MR analysis: The results of the IVW method confirmed that a total of 8 GM taxa were related to the risk of EMS. Class-Melainabacteria (p = 0.036), family-Ruminococcaceae (p = 0.037), and genus-Eubacteriumruminantium (p = 0.015) had a protective effect on EMS, whereas order-Bacillales (p = 0.046), family-Prevotellaceae (p = 0.027), genus-Anaerotruncus (p = 0.025), genus-Olsenella (p = 0.036) and genus-RuminococcaceaeUCG002 (p = 0.035) could increase the risk of EMS. (2) Sensitivity analysis: Cochrane’s Q test (p > 0.05), MR-Egger intercept method (p > 0.05), and leave-one-out method confirmed the robustness of MR results.ConclusionThis study performed a MR analysis on two large national databases and identified the association between 8 GM taxa and EMS. These taxa could potentially be utilized for indirectly diagnosing EMS and could lead to novel perspectives in research regarding the pathogenesis, diagnosis, and treatment of EMS.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188458/fullendometriosisgut microbiotaMendelian randomization analysisgenome-wide association studyinstrumental variables |
| spellingShingle | Xuan Ji Qi Yang Xiu-Lin Zhu Li Xu Jie-Ying Guo Yan Rong Yun-Lang Cai Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study Frontiers in Microbiology endometriosis gut microbiota Mendelian randomization analysis genome-wide association study instrumental variables |
| title | Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study |
| title_full | Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study |
| title_fullStr | Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study |
| title_full_unstemmed | Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study |
| title_short | Association between gut microbiota and endometriosis: a two-sample Mendelian randomization study |
| title_sort | association between gut microbiota and endometriosis a two sample mendelian randomization study |
| topic | endometriosis gut microbiota Mendelian randomization analysis genome-wide association study instrumental variables |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188458/full |
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