MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B
MicroRNA (miR)-199a-5p has been shown to function as a tumor suppressor in some malignancies but its role in esophageal cancer is poorly understood. To further explore its role in esophageal cancer, we sought to investigate the interaction between miR-199a-5p and Jun-B, an important component of the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/19/4811 |
| _version_ | 1797576076997689344 |
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| author | Pornima Phatak Mohan E. Tulapurkar Whitney M. Burrows James M. Donahue |
| author_facet | Pornima Phatak Mohan E. Tulapurkar Whitney M. Burrows James M. Donahue |
| author_sort | Pornima Phatak |
| collection | DOAJ |
| description | MicroRNA (miR)-199a-5p has been shown to function as a tumor suppressor in some malignancies but its role in esophageal cancer is poorly understood. To further explore its role in esophageal cancer, we sought to investigate the interaction between miR-199a-5p and Jun-B, an important component of the AP1 transcription factor, which contains a potential binding site for miR-199a-5p in its mRNA. We found that levels of miR-199a-5p are reduced in both human esophageal cancer specimens and in multiple esophageal cancer cell lines compared to esophageal epithelial cells. Jun-B expression is correspondingly elevated in these tumor specimens and in several cell lines compared to esophageal epithelial cells. Jun-B mRNA expression and stability, as well as protein expression, are markedly decreased following miR-199a-5p overexpression. A direct interaction between miR-199a-5p and Jun-B mRNA was confirmed by a biotinylated RNA-pull down assay and luciferase reporter constructs. Either forced expression of miR-199a-5p or Jun-B silencing led to a significant decrease in cellular proliferation as well as in AP-1 promoter activity. Our results provide evidence that miR-199a-5p functions as a tumor suppressor in esophageal cancer cells by regulating cellular proliferation, partially through repression of Jun B. |
| first_indexed | 2024-03-10T21:47:22Z |
| format | Article |
| id | doaj.art-2b882f8cd5d343259c5427b92a97c870 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T21:47:22Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-2b882f8cd5d343259c5427b92a97c8702023-11-19T14:10:55ZengMDPI AGCancers2072-66942023-09-011519481110.3390/cancers15194811MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-BPornima Phatak0Mohan E. Tulapurkar1Whitney M. Burrows2James M. Donahue3Birmingham Veterans Affairs Health Care System, Birmingham, AL 35233, USADepartment of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USADepartment of Surgery Thoracic Medicine and Surgery, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USABirmingham Veterans Affairs Health Care System, Birmingham, AL 35233, USAMicroRNA (miR)-199a-5p has been shown to function as a tumor suppressor in some malignancies but its role in esophageal cancer is poorly understood. To further explore its role in esophageal cancer, we sought to investigate the interaction between miR-199a-5p and Jun-B, an important component of the AP1 transcription factor, which contains a potential binding site for miR-199a-5p in its mRNA. We found that levels of miR-199a-5p are reduced in both human esophageal cancer specimens and in multiple esophageal cancer cell lines compared to esophageal epithelial cells. Jun-B expression is correspondingly elevated in these tumor specimens and in several cell lines compared to esophageal epithelial cells. Jun-B mRNA expression and stability, as well as protein expression, are markedly decreased following miR-199a-5p overexpression. A direct interaction between miR-199a-5p and Jun-B mRNA was confirmed by a biotinylated RNA-pull down assay and luciferase reporter constructs. Either forced expression of miR-199a-5p or Jun-B silencing led to a significant decrease in cellular proliferation as well as in AP-1 promoter activity. Our results provide evidence that miR-199a-5p functions as a tumor suppressor in esophageal cancer cells by regulating cellular proliferation, partially through repression of Jun B.https://www.mdpi.com/2072-6694/15/19/4811miR-199a-5pJun-Besophageal cancerproliferation |
| spellingShingle | Pornima Phatak Mohan E. Tulapurkar Whitney M. Burrows James M. Donahue MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B Cancers miR-199a-5p Jun-B esophageal cancer proliferation |
| title | MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B |
| title_full | MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B |
| title_fullStr | MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B |
| title_full_unstemmed | MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B |
| title_short | MiR-199a-5p Decreases Esophageal Cancer Cell Proliferation Partially through Repression of Jun-B |
| title_sort | mir 199a 5p decreases esophageal cancer cell proliferation partially through repression of jun b |
| topic | miR-199a-5p Jun-B esophageal cancer proliferation |
| url | https://www.mdpi.com/2072-6694/15/19/4811 |
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