MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
Abstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-00638-3 |
| _version_ | 1798034831554117632 |
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| author | Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang |
| author_facet | Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang |
| author_sort | Shuo Cong |
| collection | DOAJ |
| description | Abstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in patients with different progressions of liver fibrosis was determined by RT-PCR and Western blot analysis. Set up Notch3 up group and Notch3 down regulated group, RT-PCR and Western blot were used to determine the effect of Notch signaling on the expression of fibrogenic factors. CCK-8, cell scratch assays, Transwell assays, flow cytometry were used to determine the effect of miR-571 on LX-2 proliferation, migration, apoptosis in human stem stellate cells, and RT-PCR, Western blot assays were performed to determine the effect of miR-571 on the Notch3 signaling pathway and the expression of profibrogenic factors. miR-571, Notch3 and Jagged1 are up-regulated in patients with liver fibrosis and is associated with the progression of liver fibrosis. Notch3 signaling pathway can promote the expression of fibroblast in human hepatic stellate cells; miR-571 can inhibit the apoptosis of human hepatic stellate cells, promote cell proliferation and migration; up regulation of miR-571 can promote the expression of Notch3 and Jagged1, and up-regulation of miR-571 also promoted the expression of related fibroblasts. MiR-571 can promote the activation of human stem cell stellate cells and the expression of fibroblast related factors through Notch3 signaling pathway. |
| first_indexed | 2024-04-11T20:49:52Z |
| format | Article |
| id | doaj.art-2b88ef43c3104f2bb7cad42ca929c2ef |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T20:49:52Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2b88ef43c3104f2bb7cad42ca929c2ef2022-12-22T04:03:53ZengNature PortfolioScientific Reports2045-23222021-11-0111111110.1038/s41598-021-00638-3MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathwayShuo Cong0Yongmei Liu1Yi Li2Yu Chen3Rui Chen4Baofang Zhang5Lei Yu6Yaxin Hu7Xueke Zhao8Mao Mu9Mingliang Cheng10Zhi Huang11School of Basic Medicine Sciences, Guizhou Medical UniversityClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical UniversityCollege of Medical Laboratory, Guizhou Medical UniversityClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Acupuncture and Moxibustion, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Obstetrics and Gynecology, Maternal and Child Health Hospital of Guiyang ProvincePrenatal Diagnosis Center, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Guizhou Medical UniversitySchool of Basic Medicine Sciences, Guizhou Medical UniversityAbstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in patients with different progressions of liver fibrosis was determined by RT-PCR and Western blot analysis. Set up Notch3 up group and Notch3 down regulated group, RT-PCR and Western blot were used to determine the effect of Notch signaling on the expression of fibrogenic factors. CCK-8, cell scratch assays, Transwell assays, flow cytometry were used to determine the effect of miR-571 on LX-2 proliferation, migration, apoptosis in human stem stellate cells, and RT-PCR, Western blot assays were performed to determine the effect of miR-571 on the Notch3 signaling pathway and the expression of profibrogenic factors. miR-571, Notch3 and Jagged1 are up-regulated in patients with liver fibrosis and is associated with the progression of liver fibrosis. Notch3 signaling pathway can promote the expression of fibroblast in human hepatic stellate cells; miR-571 can inhibit the apoptosis of human hepatic stellate cells, promote cell proliferation and migration; up regulation of miR-571 can promote the expression of Notch3 and Jagged1, and up-regulation of miR-571 also promoted the expression of related fibroblasts. MiR-571 can promote the activation of human stem cell stellate cells and the expression of fibroblast related factors through Notch3 signaling pathway.https://doi.org/10.1038/s41598-021-00638-3 |
| spellingShingle | Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway Scientific Reports |
| title | MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway |
| title_full | MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway |
| title_fullStr | MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway |
| title_full_unstemmed | MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway |
| title_short | MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway |
| title_sort | mir 571 affects the development and progression of liver fibrosis by regulating the notch3 pathway |
| url | https://doi.org/10.1038/s41598-021-00638-3 |
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