Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans
Abstract The aging process is affected by various stressors. An increase in oxidative stress is related to the impairment of physiological functions and enhancement of glycative stress. Food‐derived bioactive peptides have various physiological functions, including antioxidant activities. Dipeptides...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3256 |
| _version_ | 1797805677603717120 |
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| author | Issei Yokoyama Ou Setoyama Yaqi Jia Nana Fujita Akane Waki Yusuke Komiya Jun Nagasao Keizo Arihara |
| author_facet | Issei Yokoyama Ou Setoyama Yaqi Jia Nana Fujita Akane Waki Yusuke Komiya Jun Nagasao Keizo Arihara |
| author_sort | Issei Yokoyama |
| collection | DOAJ |
| description | Abstract The aging process is affected by various stressors. An increase in oxidative stress is related to the impairment of physiological functions and enhancement of glycative stress. Food‐derived bioactive peptides have various physiological functions, including antioxidant activities. Dipeptides comprising Leu and Lys (LK and KL, respectively) have been isolated from foods; however, their physiological properties remain unclear. In this study, we investigated the antioxidant/antiglycation activity of dipeptides and their antiaging effects using Caenorhabditis elegans (C. elegans). Both dipeptides showed antioxidant activities against several reactive oxygen species (ROS) in vitro. In particular, the scavenging activity of LK against superoxide radicals was higher than KL did. Moreover, dipeptides suppressed advanced glycation end products (AGEs) formation in the BSA–glucose model. In the lifespan assays using wild‐type C. elegans, both LK and KL significantly prolonged the mean lifespan by 20.9% and 11.7%, respectively. In addition, LK decreased intracellular ROS and superoxide radical levels in C. elegans. Blue autofluorescence, an indicator of glycation in C. elegans with age, was also suppressed by LK. These results suggest that dipeptides, notably LK, show an antiaging effect by suppressing oxidative and glycative stress. Our findings suggest that such dipeptides can be used as a novel functional food ingredient. Food‐derived dipeptide Leu–Lys (LK) and Lys–Leu (KL) exert antioxidant and antiglycation activity in vitro. Treatment with LK prolonged the mean lifespan and maximum lifespan of C. elegans more than that of KL. Intracellular ROS and blue autofluorescence levels (indicator of aging) were suppressed by LK. |
| first_indexed | 2024-03-13T05:55:41Z |
| format | Article |
| id | doaj.art-2b8a5f29b00a47fdb1031c490e1a0bbd |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-13T05:55:41Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-2b8a5f29b00a47fdb1031c490e1a0bbd2023-06-13T05:10:23ZengWileyFood Science & Nutrition2048-71772023-06-011162776278610.1002/fsn3.3256Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegansIssei Yokoyama0Ou Setoyama1Yaqi Jia2Nana Fujita3Akane Waki4Yusuke Komiya5Jun Nagasao6Keizo Arihara7School of Veterinary Medicine Kitasato University Towada JapanKanagawa Institute of Industrial Science and Technology Ebina JapanSchool of Veterinary Medicine Kitasato University Towada JapanSchool of Veterinary Medicine Kitasato University Towada JapanSchool of Veterinary Medicine Kitasato University Towada JapanSchool of Veterinary Medicine Kitasato University Towada JapanSchool of Veterinary Medicine Kitasato University Towada JapanSchool of Veterinary Medicine Kitasato University Towada JapanAbstract The aging process is affected by various stressors. An increase in oxidative stress is related to the impairment of physiological functions and enhancement of glycative stress. Food‐derived bioactive peptides have various physiological functions, including antioxidant activities. Dipeptides comprising Leu and Lys (LK and KL, respectively) have been isolated from foods; however, their physiological properties remain unclear. In this study, we investigated the antioxidant/antiglycation activity of dipeptides and their antiaging effects using Caenorhabditis elegans (C. elegans). Both dipeptides showed antioxidant activities against several reactive oxygen species (ROS) in vitro. In particular, the scavenging activity of LK against superoxide radicals was higher than KL did. Moreover, dipeptides suppressed advanced glycation end products (AGEs) formation in the BSA–glucose model. In the lifespan assays using wild‐type C. elegans, both LK and KL significantly prolonged the mean lifespan by 20.9% and 11.7%, respectively. In addition, LK decreased intracellular ROS and superoxide radical levels in C. elegans. Blue autofluorescence, an indicator of glycation in C. elegans with age, was also suppressed by LK. These results suggest that dipeptides, notably LK, show an antiaging effect by suppressing oxidative and glycative stress. Our findings suggest that such dipeptides can be used as a novel functional food ingredient. Food‐derived dipeptide Leu–Lys (LK) and Lys–Leu (KL) exert antioxidant and antiglycation activity in vitro. Treatment with LK prolonged the mean lifespan and maximum lifespan of C. elegans more than that of KL. Intracellular ROS and blue autofluorescence levels (indicator of aging) were suppressed by LK.https://doi.org/10.1002/fsn3.3256antiagingantiglycationantioxidant activitybioactive peptideCaenorhabditis elegansdipeptide |
| spellingShingle | Issei Yokoyama Ou Setoyama Yaqi Jia Nana Fujita Akane Waki Yusuke Komiya Jun Nagasao Keizo Arihara Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans Food Science & Nutrition antiaging antiglycation antioxidant activity bioactive peptide Caenorhabditis elegans dipeptide |
| title | Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans |
| title_full | Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans |
| title_fullStr | Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans |
| title_full_unstemmed | Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans |
| title_short | Effects of the dipeptides comprising leucine and lysine on lifespan and age‐related stress in Caenorhabditis elegans |
| title_sort | effects of the dipeptides comprising leucine and lysine on lifespan and age related stress in caenorhabditis elegans |
| topic | antiaging antiglycation antioxidant activity bioactive peptide Caenorhabditis elegans dipeptide |
| url | https://doi.org/10.1002/fsn3.3256 |
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