Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines
The <i>Zika virus</i> (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described as sexually and vertically transmitted. The latter shows severe consequences for the embryo/fetus, such as congenital microcephaly and deficiency of the neu...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/7/1446 |
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| author | Márcia Duarte Barbosa Anderson Costa Paula Prieto-Oliveira Robert Andreata-Santos Cristina M. Peter Paolo M. A. Zanotto Luiz Mario Ramos Janini |
| author_facet | Márcia Duarte Barbosa Anderson Costa Paula Prieto-Oliveira Robert Andreata-Santos Cristina M. Peter Paolo M. A. Zanotto Luiz Mario Ramos Janini |
| author_sort | Márcia Duarte Barbosa |
| collection | DOAJ |
| description | The <i>Zika virus</i> (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described as sexually and vertically transmitted. The latter shows severe consequences for the embryo/fetus, such as congenital microcephaly and deficiency of the neural system, currently known as Congenital ZIKV Syndrome (CZS). To better understand ZIKV dynamics in trophoblastic cells present in the first trimester of pregnancy (BeWo, HTR-8, and control cell HuH-7), an experiment of viral kinetics was performed for African MR766 low passage and Asian-Brazilian IEC ZIKV lineages. The results were described independently and demonstrated that the three placental cells lines are permissive and susceptible to ZIKV. We noticed cytopathic effects that are typical in in vitro viral infection in BeWo and HTR-8. Regarding kinetics, MR766lp showed peaks of viral loads in 24 and 48 hpi for all cell types tested, as well as marked cells death after peak production. On the other hand, the HTR-8 lineage inoculated with ZIKV-IEC exhibited increased viral production in 144 hpi, with a peak between 24 and 96 hpi. Furthermore, IEC had peak variations of viral production for BeWo in 144 hpi. Considering such in vitro results, the hypothesis that maternal fetal transmission is probably a way of virus transmission between the mother and the embryo/fetus is maintained. |
| first_indexed | 2024-03-11T00:33:59Z |
| format | Article |
| id | doaj.art-2b8e5ae36f594f23be8485b3f3469ede |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T00:33:59Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-2b8e5ae36f594f23be8485b3f3469ede2023-11-18T21:43:49ZengMDPI AGViruses1999-49152023-06-01157144610.3390/v15071446Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell LinesMárcia Duarte Barbosa0Anderson Costa1Paula Prieto-Oliveira2Robert Andreata-Santos3Cristina M. Peter4Paolo M. A. Zanotto5Luiz Mario Ramos Janini6Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Biosciences, University of São Paulo, São Paulo 05508-000, BrazilLaboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of São Paulo, São Paulo 04039-032, BrazilDepartment of Bioinformatics and Genomics, College of Computing and Informatics, University of North Carolina at Charlotte, 9331 Robert D. Snyder Rd., Charlotte, NC 28223, USALaboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of São Paulo, São Paulo 04039-032, BrazilLaboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of São Paulo, São Paulo 04039-032, BrazilLaboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Biosciences, University of São Paulo, São Paulo 05508-000, BrazilLaboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of São Paulo, São Paulo 04039-032, BrazilThe <i>Zika virus</i> (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described as sexually and vertically transmitted. The latter shows severe consequences for the embryo/fetus, such as congenital microcephaly and deficiency of the neural system, currently known as Congenital ZIKV Syndrome (CZS). To better understand ZIKV dynamics in trophoblastic cells present in the first trimester of pregnancy (BeWo, HTR-8, and control cell HuH-7), an experiment of viral kinetics was performed for African MR766 low passage and Asian-Brazilian IEC ZIKV lineages. The results were described independently and demonstrated that the three placental cells lines are permissive and susceptible to ZIKV. We noticed cytopathic effects that are typical in in vitro viral infection in BeWo and HTR-8. Regarding kinetics, MR766lp showed peaks of viral loads in 24 and 48 hpi for all cell types tested, as well as marked cells death after peak production. On the other hand, the HTR-8 lineage inoculated with ZIKV-IEC exhibited increased viral production in 144 hpi, with a peak between 24 and 96 hpi. Furthermore, IEC had peak variations of viral production for BeWo in 144 hpi. Considering such in vitro results, the hypothesis that maternal fetal transmission is probably a way of virus transmission between the mother and the embryo/fetus is maintained.https://www.mdpi.com/1999-4915/15/7/1446<i>Zika virus</i>trophoblast cellviral kinetics |
| spellingShingle | Márcia Duarte Barbosa Anderson Costa Paula Prieto-Oliveira Robert Andreata-Santos Cristina M. Peter Paolo M. A. Zanotto Luiz Mario Ramos Janini Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines Viruses <i>Zika virus</i> trophoblast cell viral kinetics |
| title | Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines |
| title_full | Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines |
| title_fullStr | Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines |
| title_full_unstemmed | Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines |
| title_short | Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—<i>Zika virus</i> Strains (Step Growth Curve) in Trophoblastic Cell Lines |
| title_sort | proposal of model for evaluation of viral kinetics of african asian brazilian i zika virus i strains step growth curve in trophoblastic cell lines |
| topic | <i>Zika virus</i> trophoblast cell viral kinetics |
| url | https://www.mdpi.com/1999-4915/15/7/1446 |
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