Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection
The temperature effect has been discussed in the past on pH sensors. In this study, the temperature effect is analyzed for the ascorbic acid (AA) biosensor based on enzymatic ascorbate oxidase (AO). To reduce this effect, a novel temperature compensation circuit was implemented by Taiwan Semiconduct...
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| Format: | Article |
| Language: | English |
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IEEE
2022-01-01
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| Series: | IEEE Journal of the Electron Devices Society |
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| Online Access: | https://ieeexplore.ieee.org/document/9854054/ |
| _version_ | 1828434081125236736 |
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| author | Po-Yu Kuo Wei-Hao Lai Chun-Hung Chang Tai-Hui Wang |
| author_facet | Po-Yu Kuo Wei-Hao Lai Chun-Hung Chang Tai-Hui Wang |
| author_sort | Po-Yu Kuo |
| collection | DOAJ |
| description | The temperature effect has been discussed in the past on pH sensors. In this study, the temperature effect is analyzed for the ascorbic acid (AA) biosensor based on enzymatic ascorbate oxidase (AO). To reduce this effect, a novel temperature compensation circuit was implemented by Taiwan Semiconductor Manufacturing Co., Ltd. (TSMC) 180 nm complementary metal-oxide-semiconductor (CMOS) process. By applying the proposed circuit, the temperature coefficients (TCs) with five concentrations of AA were suppressed below <inline-formula> <tex-math notation="LaTeX">$201~\mathbf {\mu }\text{V}/\mathbf {^{\circ} }\text{C}$ </tex-math></inline-formula>. The TC of the normal level of AA concentration (0.0312 mM) in the human body was <inline-formula> <tex-math notation="LaTeX">$188~\mathbf {\mu }\text{V}/\mathbf {^{\circ} }\text{C}$ </tex-math></inline-formula>, at the temperature range from 25°C to 55°C. The polyethylene terephthalate (PET) substrate was utilized. To confirm the stability of the fabricated AO/RuO<sub>2</sub>/PET AA biosensor, interference and hysteresis experiments were carried out. At present, the experiments were still in the stage of in vitro measurement. The results showed that the AO/RuO<sub>2</sub>/PET AA biosensor had good selectivity that effective against other interference that may exist in blood at either 25 °C or 37.5°C. Moreover, at the temperature of 37.5°C, the hysteresis voltage of the biosensor after applying the compensation circuit was reduced from 18.27 mV to 1.39 mV. |
| first_indexed | 2024-12-10T18:45:13Z |
| format | Article |
| id | doaj.art-2b91412a710e4a55ae056e77b0769d53 |
| institution | Directory Open Access Journal |
| issn | 2168-6734 |
| language | English |
| last_indexed | 2024-12-10T18:45:13Z |
| publishDate | 2022-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Journal of the Electron Devices Society |
| spelling | doaj.art-2b91412a710e4a55ae056e77b0769d532022-12-22T01:37:32ZengIEEEIEEE Journal of the Electron Devices Society2168-67342022-01-011067067810.1109/JEDS.2022.31978309854054Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid DetectionPo-Yu Kuo0https://orcid.org/0000-0003-2791-9642Wei-Hao Lai1https://orcid.org/0000-0002-6637-8896Chun-Hung Chang2https://orcid.org/0000-0002-2601-6874Tai-Hui Wang3Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, TaiwanThe temperature effect has been discussed in the past on pH sensors. In this study, the temperature effect is analyzed for the ascorbic acid (AA) biosensor based on enzymatic ascorbate oxidase (AO). To reduce this effect, a novel temperature compensation circuit was implemented by Taiwan Semiconductor Manufacturing Co., Ltd. (TSMC) 180 nm complementary metal-oxide-semiconductor (CMOS) process. By applying the proposed circuit, the temperature coefficients (TCs) with five concentrations of AA were suppressed below <inline-formula> <tex-math notation="LaTeX">$201~\mathbf {\mu }\text{V}/\mathbf {^{\circ} }\text{C}$ </tex-math></inline-formula>. The TC of the normal level of AA concentration (0.0312 mM) in the human body was <inline-formula> <tex-math notation="LaTeX">$188~\mathbf {\mu }\text{V}/\mathbf {^{\circ} }\text{C}$ </tex-math></inline-formula>, at the temperature range from 25°C to 55°C. The polyethylene terephthalate (PET) substrate was utilized. To confirm the stability of the fabricated AO/RuO<sub>2</sub>/PET AA biosensor, interference and hysteresis experiments were carried out. At present, the experiments were still in the stage of in vitro measurement. The results showed that the AO/RuO<sub>2</sub>/PET AA biosensor had good selectivity that effective against other interference that may exist in blood at either 25 °C or 37.5°C. Moreover, at the temperature of 37.5°C, the hysteresis voltage of the biosensor after applying the compensation circuit was reduced from 18.27 mV to 1.39 mV.https://ieeexplore.ieee.org/document/9854054/Temperature effecttemperature compensation circuitascorbic acid (AA)ruthenium dioxide (RuO₂)enzymatic biosensorCMOS process |
| spellingShingle | Po-Yu Kuo Wei-Hao Lai Chun-Hung Chang Tai-Hui Wang Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection IEEE Journal of the Electron Devices Society Temperature effect temperature compensation circuit ascorbic acid (AA) ruthenium dioxide (RuO₂) enzymatic biosensor CMOS process |
| title | Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection |
| title_full | Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection |
| title_fullStr | Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection |
| title_full_unstemmed | Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection |
| title_short | Temperature Effect Analysis of the Enzymatic RuO<sub>2</sub> Biomedical Sensor for Ascorbic Acid Detection |
| title_sort | temperature effect analysis of the enzymatic ruo sub 2 sub biomedical sensor for ascorbic acid detection |
| topic | Temperature effect temperature compensation circuit ascorbic acid (AA) ruthenium dioxide (RuO₂) enzymatic biosensor CMOS process |
| url | https://ieeexplore.ieee.org/document/9854054/ |
| work_keys_str_mv | AT poyukuo temperatureeffectanalysisoftheenzymaticruosub2subbiomedicalsensorforascorbicaciddetection AT weihaolai temperatureeffectanalysisoftheenzymaticruosub2subbiomedicalsensorforascorbicaciddetection AT chunhungchang temperatureeffectanalysisoftheenzymaticruosub2subbiomedicalsensorforascorbicaciddetection AT taihuiwang temperatureeffectanalysisoftheenzymaticruosub2subbiomedicalsensorforascorbicaciddetection |