Three-dimensional multi-site random access photostimulation (3D-MAP)
Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/73266 |
| _version_ | 1811180338356944896 |
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| author | Yi Xue Laura Waller Hillel Adesnik Nicolas Pégard |
| author_facet | Yi Xue Laura Waller Hillel Adesnik Nicolas Pégard |
| author_sort | Yi Xue |
| collection | DOAJ |
| description | Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost. |
| first_indexed | 2024-04-11T09:01:27Z |
| format | Article |
| id | doaj.art-2b97d649d2754656b95ef0ad9eca366f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:01:27Z |
| publishDate | 2022-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2b97d649d2754656b95ef0ad9eca366f2022-12-22T04:32:46ZengeLife Sciences Publications LtdeLife2050-084X2022-02-011110.7554/eLife.73266Three-dimensional multi-site random access photostimulation (3D-MAP)Yi Xue0https://orcid.org/0000-0003-2622-083XLaura Waller1https://orcid.org/0000-0003-1243-2356Hillel Adesnik2https://orcid.org/0000-0002-3796-8643Nicolas Pégard3https://orcid.org/0000-0003-2868-7118Department of Electrical Engineering & Computer Sciences, University of California, Berkeley, Berkeley, United StatesDepartment of Electrical Engineering & Computer Sciences, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular & Cell Biology, University of California, Berkeley, Berkeley, United States; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, United StatesDepartment of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, United States; Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, United States; UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, United StatesOptical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost.https://elifesciences.org/articles/73266light fieldoptogeneticscalcium imagingoptical microscopyneural circuitvisual cortex |
| spellingShingle | Yi Xue Laura Waller Hillel Adesnik Nicolas Pégard Three-dimensional multi-site random access photostimulation (3D-MAP) eLife light field optogenetics calcium imaging optical microscopy neural circuit visual cortex |
| title | Three-dimensional multi-site random access photostimulation (3D-MAP) |
| title_full | Three-dimensional multi-site random access photostimulation (3D-MAP) |
| title_fullStr | Three-dimensional multi-site random access photostimulation (3D-MAP) |
| title_full_unstemmed | Three-dimensional multi-site random access photostimulation (3D-MAP) |
| title_short | Three-dimensional multi-site random access photostimulation (3D-MAP) |
| title_sort | three dimensional multi site random access photostimulation 3d map |
| topic | light field optogenetics calcium imaging optical microscopy neural circuit visual cortex |
| url | https://elifesciences.org/articles/73266 |
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