The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM]
Background: MicroRNAs (miRNAs) are small RNAs that regulate gene expression by suppressing protein translation and may influence RNA expression. MicroRNAs are detected in extracellular locations such as plasma; however, the extent of miRNA expression in plasma its relation to cardiovascular disease...
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F1000 Research Ltd
2012-11-01
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| Online Access: | http://f1000research.com/articles/1-50/v1 |
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| author | Jane E Freedman Bahadir Ercan Kristine M Morin Ching-Ti Liu Lulufer Tamer Lokman Ayaz Mehmet Kanadasi Dilek Cicek Ali Ihsan Seyhan Rabia Eker Akilli Celalettin Camci Beyhan Cengiz Serdar Oztuzcu Kahraman Tanriverdi |
| author_facet | Jane E Freedman Bahadir Ercan Kristine M Morin Ching-Ti Liu Lulufer Tamer Lokman Ayaz Mehmet Kanadasi Dilek Cicek Ali Ihsan Seyhan Rabia Eker Akilli Celalettin Camci Beyhan Cengiz Serdar Oztuzcu Kahraman Tanriverdi |
| author_sort | Jane E Freedman |
| collection | DOAJ |
| description | Background: MicroRNAs (miRNAs) are small RNAs that regulate gene expression by suppressing protein translation and may influence RNA expression. MicroRNAs are detected in extracellular locations such as plasma; however, the extent of miRNA expression in plasma its relation to cardiovascular disease is not clear and many clinical studies have utilized array-based platforms with poor reproducibility. Methods and Results: Initially, to define distribution of miRNA in human blood; whole blood, platelets, mononuclear cells, plasma, and serum from 5 normal individuals were screened for 852 miRNAs using high-throughput micro-fluidic quantitative RT-PCR (qRT-PCR). In total; 609, 448, 658, 147, and 178 miRNAs were found to be expressed in moderate to high levels in whole blood, platelets, mononuclear cells, plasma, and serum, respectively, with some miRNAs uniquely expressed. To determine the cardiovascular relevance of blood miRNA expression, plasma miRNA (n=852) levels were measured in 83 patients presenting for cardiac catheterization. Eight plasma miRNAs were found to have over 2-fold increased expression in patients with significant coronary disease (≥70% stenosis) as compared to those with minimal coronary disease (less than 70% stenosis) or normal coronary arteries. Expression of miR-494, miR-490-3p, and miR-769-3p were found to have significantly different levels of expression. Using a multivariable regression model including cardiovascular risk factors and medications, hsa-miR-769-3p was found to be significantly correlated with the presence of significant coronary atherosclerosis. Conclusions: This study utilized a superior high-throughput qRT-PCR based method and found that miRNAs are found to be widely expressed in human blood with differences expressed between cellular and extracellular fractions. Importantly, specific miRNAs from circulating plasma are associated with the presence of significant coronary disease. |
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| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-23T11:07:44Z |
| publishDate | 2012-11-01 |
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| spelling | doaj.art-2b9bbcd42fdc4a3299a6ca3d52e81f812022-12-21T17:49:26ZengF1000 Research LtdF1000Research2046-14022012-11-01110.12688/f1000research.1-50.v1221The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM]Jane E Freedman0Bahadir Ercan1Kristine M Morin2Ching-Ti Liu3Lulufer Tamer4Lokman Ayaz5Mehmet Kanadasi6Dilek Cicek7Ali Ihsan Seyhan8Rabia Eker Akilli9Celalettin Camci10Beyhan Cengiz11Serdar Oztuzcu12Kahraman Tanriverdi13Department of Medicine, University of Massachusetts Medical School, Massachusetts, 01605, USADepartment of Medicine, University of Massachusetts Medical School, Massachusetts, 01605, USADepartment of Medicine, University of Massachusetts Medical School, Massachusetts, 01605, USADepartment of Biostatistics, School of Public Health, Boston University, Boston, 02118, USADepartment of Biochemistry, Mersin University, School of Medicine, Mersin, 33343, TurkeyDepartment of Biochemistry, Mersin University, School of Medicine, Mersin, 33343, TurkeyDepartment of Cardiology, Cukurova University, School of Medicine, Adana, 01110, TurkeyDepartment of Cardiology, Mersin University, School of Medicine, Mersin, 33343, TurkeyDepartment of Cardiology, Mersin University, School of Medicine, Mersin, 33343, TurkeyDepartment of Cardiology, Cukurova University, School of Medicine, Adana, 01110, TurkeyDepartment of Oncology, Gaziantep University, School of Medicine, Gazientep, 27310, TurkeyDepartment of Physiology, Gaziantep University, School of Medicine, Gazientep, 27310, TurkeySahinbey Hospital Central Laboratory, Gaziantep University, Gaziantep, 27310, TurkeyDepartment of Medicine, University of Massachusetts Medical School, Massachusetts, 01605, USABackground: MicroRNAs (miRNAs) are small RNAs that regulate gene expression by suppressing protein translation and may influence RNA expression. MicroRNAs are detected in extracellular locations such as plasma; however, the extent of miRNA expression in plasma its relation to cardiovascular disease is not clear and many clinical studies have utilized array-based platforms with poor reproducibility. Methods and Results: Initially, to define distribution of miRNA in human blood; whole blood, platelets, mononuclear cells, plasma, and serum from 5 normal individuals were screened for 852 miRNAs using high-throughput micro-fluidic quantitative RT-PCR (qRT-PCR). In total; 609, 448, 658, 147, and 178 miRNAs were found to be expressed in moderate to high levels in whole blood, platelets, mononuclear cells, plasma, and serum, respectively, with some miRNAs uniquely expressed. To determine the cardiovascular relevance of blood miRNA expression, plasma miRNA (n=852) levels were measured in 83 patients presenting for cardiac catheterization. Eight plasma miRNAs were found to have over 2-fold increased expression in patients with significant coronary disease (≥70% stenosis) as compared to those with minimal coronary disease (less than 70% stenosis) or normal coronary arteries. Expression of miR-494, miR-490-3p, and miR-769-3p were found to have significantly different levels of expression. Using a multivariable regression model including cardiovascular risk factors and medications, hsa-miR-769-3p was found to be significantly correlated with the presence of significant coronary atherosclerosis. Conclusions: This study utilized a superior high-throughput qRT-PCR based method and found that miRNAs are found to be widely expressed in human blood with differences expressed between cellular and extracellular fractions. Importantly, specific miRNAs from circulating plasma are associated with the presence of significant coronary disease.http://f1000research.com/articles/1-50/v1Cardiovascular Physiology/CirculationControl of Gene ExpressionMedical Genetics |
| spellingShingle | Jane E Freedman Bahadir Ercan Kristine M Morin Ching-Ti Liu Lulufer Tamer Lokman Ayaz Mehmet Kanadasi Dilek Cicek Ali Ihsan Seyhan Rabia Eker Akilli Celalettin Camci Beyhan Cengiz Serdar Oztuzcu Kahraman Tanriverdi The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] F1000Research Cardiovascular Physiology/Circulation Control of Gene Expression Medical Genetics |
| title | The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] |
| title_full | The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] |
| title_fullStr | The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] |
| title_full_unstemmed | The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] |
| title_short | The distribution of circulating microRNA and their relation to coronary disease [v1; ref status: indexed, http://f1000r.es/SZ9aqM] |
| title_sort | distribution of circulating microrna and their relation to coronary disease v1 ref status indexed http f1000r es sz9aqm |
| topic | Cardiovascular Physiology/Circulation Control of Gene Expression Medical Genetics |
| url | http://f1000research.com/articles/1-50/v1 |
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