Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate
The intensive global use of glyphosate has led to the evolution of glyphosate resistant (GR) weed species, including the economically damaging horseweed (<i>Conyza sumatrensis</i>). We evaluated the glyphosate resistance mechanisms of <i>C. sumatrensis</i>. While 5-enolpyruvy...
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MDPI AG
2022-12-01
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| author | Gabrielly Cristina Kubis Raizza Zorman Marques Rafael Shinji Akiyama Kitamura Arthur Arrobas Martins Barroso Philippe Juneau Marcelo Pedrosa Gomes |
| author_facet | Gabrielly Cristina Kubis Raizza Zorman Marques Rafael Shinji Akiyama Kitamura Arthur Arrobas Martins Barroso Philippe Juneau Marcelo Pedrosa Gomes |
| author_sort | Gabrielly Cristina Kubis |
| collection | DOAJ |
| description | The intensive global use of glyphosate has led to the evolution of glyphosate resistant (GR) weed species, including the economically damaging horseweed (<i>Conyza sumatrensis</i>). We evaluated the glyphosate resistance mechanisms of <i>C. sumatrensis</i>. While 5-enolpyruvylshikimate-3-phosphate synthase activity was similar between the glyphosate resistant (GR) and nonresistant biotypes, plants from the GR population accumulated lower shikimate levels than susceptible ones, suggesting the absence of target-site resistance mechanisms. Decreases over time in glyphosate concentrations in GR leaves were not accompanied by increases in glyphosate concentrations in their stem and roots, indicating lower glyphosate distribution rates in GR plants. The early appearance of aminomethylphosphonic acid (the main glyphosate metabolite) in leaves, as well as its presence only in the stems and roots of GR plants, suggests faster glyphosate metabolism in GR plants than in susceptible ones. GR plants treated with glyphosate also showed greater antioxidant (ascorbate peroxidase [APX] and catalase [CAT]) and cytochrome P450-enzyme activities, indicating their great capacity to avoid glyphosate-induced oxidative stress. Three non-target mechanisms (reduced glyphosate translocation, increased metabolism, and increased antioxidant activity) therefore confer glyphosate resistance in <i>C. sumatrensis</i> plants. This is the first time that APX, CAT and P450-enzyme activities are related to GR in <i>C. sumatrensis</i>. |
| first_indexed | 2024-03-11T05:54:06Z |
| format | Article |
| id | doaj.art-2b9ee4da0b65497e84fdfea109512a0b |
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| issn | 2673-7140 |
| language | English |
| last_indexed | 2024-03-11T05:54:06Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-2b9ee4da0b65497e84fdfea109512a0b2023-11-17T13:54:10ZengMDPI AGStresses2673-71402022-12-0131475710.3390/stresses3010005Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to GlyphosateGabrielly Cristina Kubis0Raizza Zorman Marques1Rafael Shinji Akiyama Kitamura2Arthur Arrobas Martins Barroso3Philippe Juneau4Marcelo Pedrosa Gomes5Laboratório de Fisiologia de Plantas Sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba 80060-000, BrazilLaboratório de Fisiologia de Plantas Sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba 80060-000, BrazilLaboratório de Fisiologia de Plantas Sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba 80060-000, BrazilLaboratório de Defesa Sanitária Vegetal e Controle de Plantas Daninhas, Departamento de Fitotecnia e Fitossanidade, Setor de Ciências Agrárias, Universidade Federal do Paraná, Curitiba 80060-000, BrazilEcotoxicology of Aquatic Microorganisms Laboratory, GRIL, EcotoQ, TOXEN, Department of Biological Sciences, Université du Québecà Montréal, Montréal QC H2L 2C4, CanadaLaboratório de Fisiologia de Plantas Sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba 80060-000, BrazilThe intensive global use of glyphosate has led to the evolution of glyphosate resistant (GR) weed species, including the economically damaging horseweed (<i>Conyza sumatrensis</i>). We evaluated the glyphosate resistance mechanisms of <i>C. sumatrensis</i>. While 5-enolpyruvylshikimate-3-phosphate synthase activity was similar between the glyphosate resistant (GR) and nonresistant biotypes, plants from the GR population accumulated lower shikimate levels than susceptible ones, suggesting the absence of target-site resistance mechanisms. Decreases over time in glyphosate concentrations in GR leaves were not accompanied by increases in glyphosate concentrations in their stem and roots, indicating lower glyphosate distribution rates in GR plants. The early appearance of aminomethylphosphonic acid (the main glyphosate metabolite) in leaves, as well as its presence only in the stems and roots of GR plants, suggests faster glyphosate metabolism in GR plants than in susceptible ones. GR plants treated with glyphosate also showed greater antioxidant (ascorbate peroxidase [APX] and catalase [CAT]) and cytochrome P450-enzyme activities, indicating their great capacity to avoid glyphosate-induced oxidative stress. Three non-target mechanisms (reduced glyphosate translocation, increased metabolism, and increased antioxidant activity) therefore confer glyphosate resistance in <i>C. sumatrensis</i> plants. This is the first time that APX, CAT and P450-enzyme activities are related to GR in <i>C. sumatrensis</i>.https://www.mdpi.com/2673-7140/3/1/5aminomethylphosphonic aciddegradationherbicideshikimateweed |
| spellingShingle | Gabrielly Cristina Kubis Raizza Zorman Marques Rafael Shinji Akiyama Kitamura Arthur Arrobas Martins Barroso Philippe Juneau Marcelo Pedrosa Gomes Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate Stresses aminomethylphosphonic acid degradation herbicide shikimate weed |
| title | Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate |
| title_full | Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate |
| title_fullStr | Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate |
| title_full_unstemmed | Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate |
| title_short | Antioxidant Enzyme and Cytochrome P450 Activities Are Involved in Horseweed (<i>Conyza Sumatrensis</i>) Resistance to Glyphosate |
| title_sort | antioxidant enzyme and cytochrome p450 activities are involved in horseweed i conyza sumatrensis i resistance to glyphosate |
| topic | aminomethylphosphonic acid degradation herbicide shikimate weed |
| url | https://www.mdpi.com/2673-7140/3/1/5 |
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