Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera

Complicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fi...

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Main Authors: Anna L. Kaysheva, Tatyana O. Pleshakova, Alexander A. Stepanov, Vadim S. Ziborov, Shanmuga S. Saravanabhavan, Balasubramanian Natesan, Alexander I. Archakov, Yurii D. Ivanov
Format: Article
Language:English
Published: Elsevier 2019-08-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340919305943
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author Anna L. Kaysheva
Tatyana O. Pleshakova
Alexander A. Stepanov
Vadim S. Ziborov
Shanmuga S. Saravanabhavan
Balasubramanian Natesan
Alexander I. Archakov
Yurii D. Ivanov
author_facet Anna L. Kaysheva
Tatyana O. Pleshakova
Alexander A. Stepanov
Vadim S. Ziborov
Shanmuga S. Saravanabhavan
Balasubramanian Natesan
Alexander I. Archakov
Yurii D. Ivanov
author_sort Anna L. Kaysheva
collection DOAJ
description Complicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fishing is unique among other analytical protein detection approaches; it allows visualization and counting of single protein molecules [3–6]. The present dataset focus on mass spectrometry method for detection of human hepatitis C virus core antigen (HCV core Ag) taking into account the potential modification with cations in blood serum samples, using mica chips for the atomic force microscopy (AFM-chips). To conduct specific protein fishing, we used flat AFM-chips preliminary sensibilized with molecular probes – aptamers, which are single-stranded DNA sequences. In our study we used four types of aptamers up to 85 nucleotides specific against the target protein – HCVcoreAg [3,4]. Working (n = 19) and control (n = 11) AFM-chips with aptamers were preliminarily immobilized on the surface in four zones and incubated in blood serum samples (See Supplementary fig. 1). Analysis of MS data regarding modification of marker protein peptides with Na+, K+, K2Cl+, and Na2Cl + ions enables to enhance the reliability of target proteins detection in the serum thereby demonstrating a high diagnostic potential. Keywords: HCVcoreAg, Aptamer, Atomic force microscopy, Mass spectrometry, Biospecific fishing, MALDI-MS
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spelling doaj.art-2b9fd772d88a45299875cf27710a13ad2022-12-22T02:23:54ZengElsevierData in Brief2352-34092019-08-0125Immuno-MALDI MS dataset for improved detection of HCVcoreAg in seraAnna L. Kaysheva0Tatyana O. Pleshakova1Alexander A. Stepanov2Vadim S. Ziborov3Shanmuga S. Saravanabhavan4Balasubramanian Natesan5Alexander I. Archakov6Yurii D. Ivanov7Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 Russia; Corresponding author.Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaDepartment of Chemical Engineering, Anna University, Chennai, 600025, IndiaDepartment of Chemical Engineering, Anna University, Chennai, 600025, IndiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaComplicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fishing is unique among other analytical protein detection approaches; it allows visualization and counting of single protein molecules [3–6]. The present dataset focus on mass spectrometry method for detection of human hepatitis C virus core antigen (HCV core Ag) taking into account the potential modification with cations in blood serum samples, using mica chips for the atomic force microscopy (AFM-chips). To conduct specific protein fishing, we used flat AFM-chips preliminary sensibilized with molecular probes – aptamers, which are single-stranded DNA sequences. In our study we used four types of aptamers up to 85 nucleotides specific against the target protein – HCVcoreAg [3,4]. Working (n = 19) and control (n = 11) AFM-chips with aptamers were preliminarily immobilized on the surface in four zones and incubated in blood serum samples (See Supplementary fig. 1). Analysis of MS data regarding modification of marker protein peptides with Na+, K+, K2Cl+, and Na2Cl + ions enables to enhance the reliability of target proteins detection in the serum thereby demonstrating a high diagnostic potential. Keywords: HCVcoreAg, Aptamer, Atomic force microscopy, Mass spectrometry, Biospecific fishing, MALDI-MShttp://www.sciencedirect.com/science/article/pii/S2352340919305943
spellingShingle Anna L. Kaysheva
Tatyana O. Pleshakova
Alexander A. Stepanov
Vadim S. Ziborov
Shanmuga S. Saravanabhavan
Balasubramanian Natesan
Alexander I. Archakov
Yurii D. Ivanov
Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
Data in Brief
title Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
title_full Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
title_fullStr Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
title_full_unstemmed Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
title_short Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
title_sort immuno maldi ms dataset for improved detection of hcvcoreag in sera
url http://www.sciencedirect.com/science/article/pii/S2352340919305943
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