Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera
Complicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fi...
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Elsevier
2019-08-01
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Series: | Data in Brief |
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author | Anna L. Kaysheva Tatyana O. Pleshakova Alexander A. Stepanov Vadim S. Ziborov Shanmuga S. Saravanabhavan Balasubramanian Natesan Alexander I. Archakov Yurii D. Ivanov |
author_facet | Anna L. Kaysheva Tatyana O. Pleshakova Alexander A. Stepanov Vadim S. Ziborov Shanmuga S. Saravanabhavan Balasubramanian Natesan Alexander I. Archakov Yurii D. Ivanov |
author_sort | Anna L. Kaysheva |
collection | DOAJ |
description | Complicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fishing is unique among other analytical protein detection approaches; it allows visualization and counting of single protein molecules [3–6]. The present dataset focus on mass spectrometry method for detection of human hepatitis C virus core antigen (HCV core Ag) taking into account the potential modification with cations in blood serum samples, using mica chips for the atomic force microscopy (AFM-chips). To conduct specific protein fishing, we used flat AFM-chips preliminary sensibilized with molecular probes – aptamers, which are single-stranded DNA sequences. In our study we used four types of aptamers up to 85 nucleotides specific against the target protein – HCVcoreAg [3,4]. Working (n = 19) and control (n = 11) AFM-chips with aptamers were preliminarily immobilized on the surface in four zones and incubated in blood serum samples (See Supplementary fig. 1). Analysis of MS data regarding modification of marker protein peptides with Na+, K+, K2Cl+, and Na2Cl + ions enables to enhance the reliability of target proteins detection in the serum thereby demonstrating a high diagnostic potential. Keywords: HCVcoreAg, Aptamer, Atomic force microscopy, Mass spectrometry, Biospecific fishing, MALDI-MS |
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language | English |
last_indexed | 2024-04-13T23:55:23Z |
publishDate | 2019-08-01 |
publisher | Elsevier |
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series | Data in Brief |
spelling | doaj.art-2b9fd772d88a45299875cf27710a13ad2022-12-22T02:23:54ZengElsevierData in Brief2352-34092019-08-0125Immuno-MALDI MS dataset for improved detection of HCVcoreAg in seraAnna L. Kaysheva0Tatyana O. Pleshakova1Alexander A. Stepanov2Vadim S. Ziborov3Shanmuga S. Saravanabhavan4Balasubramanian Natesan5Alexander I. Archakov6Yurii D. Ivanov7Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 Russia; Corresponding author.Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaDepartment of Chemical Engineering, Anna University, Chennai, 600025, IndiaDepartment of Chemical Engineering, Anna University, Chennai, 600025, IndiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaInstitute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121 RussiaComplicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fishing is unique among other analytical protein detection approaches; it allows visualization and counting of single protein molecules [3–6]. The present dataset focus on mass spectrometry method for detection of human hepatitis C virus core antigen (HCV core Ag) taking into account the potential modification with cations in blood serum samples, using mica chips for the atomic force microscopy (AFM-chips). To conduct specific protein fishing, we used flat AFM-chips preliminary sensibilized with molecular probes – aptamers, which are single-stranded DNA sequences. In our study we used four types of aptamers up to 85 nucleotides specific against the target protein – HCVcoreAg [3,4]. Working (n = 19) and control (n = 11) AFM-chips with aptamers were preliminarily immobilized on the surface in four zones and incubated in blood serum samples (See Supplementary fig. 1). Analysis of MS data regarding modification of marker protein peptides with Na+, K+, K2Cl+, and Na2Cl + ions enables to enhance the reliability of target proteins detection in the serum thereby demonstrating a high diagnostic potential. Keywords: HCVcoreAg, Aptamer, Atomic force microscopy, Mass spectrometry, Biospecific fishing, MALDI-MShttp://www.sciencedirect.com/science/article/pii/S2352340919305943 |
spellingShingle | Anna L. Kaysheva Tatyana O. Pleshakova Alexander A. Stepanov Vadim S. Ziborov Shanmuga S. Saravanabhavan Balasubramanian Natesan Alexander I. Archakov Yurii D. Ivanov Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera Data in Brief |
title | Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera |
title_full | Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera |
title_fullStr | Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera |
title_full_unstemmed | Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera |
title_short | Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera |
title_sort | immuno maldi ms dataset for improved detection of hcvcoreag in sera |
url | http://www.sciencedirect.com/science/article/pii/S2352340919305943 |
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