Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants
In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values...
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| Format: | Article |
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Faculty of Technology, Novi Sad
2009-01-01
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| Series: | Acta Periodica Technologica |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2009/1450-71880940079P.pdf |
| _version_ | 1819033795272114176 |
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| author | Leeuw Klaas-Jan Palić Dragan V. |
| author_facet | Leeuw Klaas-Jan Palić Dragan V. |
| author_sort | Leeuw Klaas-Jan |
| collection | DOAJ |
| description | In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values obtained in vivo and using T&T, GP and EDOM techniques were 684, 716, 685 and 710 g OM/kgDM respectively and did not differ significantly (P>0.05). The obtained in vitro results were regressed against determined in-vivo values to derive prediction equations. Using the T&T technique, the prediction equation OMD (in_vivo) = -17.36 + 0.98 x OMD (in_vitro_T&T), (R2 = 0.75; RMSE = 37.59) has been obtained. The equation OMD (in_vivo) = 198.98 + 0.71 x OMD (in_vitro_GP), (R2 = 0.21; RMSE = 66.36) has been derived for Gas production procedure, while the equation OMD (in_vivo) = 102 + 0.82 x OMD (in_vitro_EDOM), (R2 = 0.86; RMSE = 27.30) has been generated for multi-enzyme incubation technique. The results of this study showed that the OMD of complete diets for ruminants can be successfully determined, and in-vivo values predicted, using multi-enzyme incubation procedure, which is important because of the fact that rumen liquor, needed for the in-vitro twostage T&T and GP techniques is not always available to analytical laboratories. |
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| format | Article |
| id | doaj.art-2ba823b97b6d40d0a1fa12e976cb8d70 |
| institution | Directory Open Access Journal |
| issn | 1450-7188 |
| language | English |
| last_indexed | 2024-12-21T07:23:31Z |
| publishDate | 2009-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-2ba823b97b6d40d0a1fa12e976cb8d702022-12-21T19:11:44ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882009-01-01200940798610.2298/APT0940079PComparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminantsLeeuw Klaas-JanPalić Dragan V.In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values obtained in vivo and using T&T, GP and EDOM techniques were 684, 716, 685 and 710 g OM/kgDM respectively and did not differ significantly (P>0.05). The obtained in vitro results were regressed against determined in-vivo values to derive prediction equations. Using the T&T technique, the prediction equation OMD (in_vivo) = -17.36 + 0.98 x OMD (in_vitro_T&T), (R2 = 0.75; RMSE = 37.59) has been obtained. The equation OMD (in_vivo) = 198.98 + 0.71 x OMD (in_vitro_GP), (R2 = 0.21; RMSE = 66.36) has been derived for Gas production procedure, while the equation OMD (in_vivo) = 102 + 0.82 x OMD (in_vitro_EDOM), (R2 = 0.86; RMSE = 27.30) has been generated for multi-enzyme incubation technique. The results of this study showed that the OMD of complete diets for ruminants can be successfully determined, and in-vivo values predicted, using multi-enzyme incubation procedure, which is important because of the fact that rumen liquor, needed for the in-vitro twostage T&T and GP techniques is not always available to analytical laboratories.http://www.doiserbia.nb.rs/img/doi/1450-7188/2009/1450-71880940079P.pdfRuminantscomplete dietsorganic matter digestibilityin vitrotechniquesprediction |
| spellingShingle | Leeuw Klaas-Jan Palić Dragan V. Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants Acta Periodica Technologica Ruminants complete diets organic matter digestibility in vitrotechniques prediction |
| title | Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| title_full | Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| title_fullStr | Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| title_full_unstemmed | Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| title_short | Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| title_sort | comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants |
| topic | Ruminants complete diets organic matter digestibility in vitrotechniques prediction |
| url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2009/1450-71880940079P.pdf |
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