Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present stu...
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MDPI AG
2021-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/6/2087 |
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| author | Mizuki Ogawa Miyako Udono Kiichiro Teruya Norihisa Uehara Yoshinori Katakura |
| author_facet | Mizuki Ogawa Miyako Udono Kiichiro Teruya Norihisa Uehara Yoshinori Katakura |
| author_sort | Mizuki Ogawa |
| collection | DOAJ |
| description | Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth. |
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| id | doaj.art-2bac127e02f948f0b8d56e1ebbc84fdc |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T10:17:19Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
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| series | Nutrients |
| spelling | doaj.art-2bac127e02f948f0b8d56e1ebbc84fdc2023-11-22T00:39:51ZengMDPI AGNutrients2072-66432021-06-01136208710.3390/nu13062087Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth PromotionMizuki Ogawa0Miyako Udono1Kiichiro Teruya2Norihisa Uehara3Yoshinori Katakura4Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, JapanFaculty of Agriculture, Kyushu University, Fukuoka 819-0395, JapanGraduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, JapanDepartment of Molecular Cell Biology and Oral Anatomy, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanGraduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, JapanEnhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth.https://www.mdpi.com/2072-6643/13/6/2087telomerase reverse transcriptasekeratinocyte–hair follicle stem cell interactionexosomestelogen–anagen transitionhair cycle regulation |
| spellingShingle | Mizuki Ogawa Miyako Udono Kiichiro Teruya Norihisa Uehara Yoshinori Katakura Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion Nutrients telomerase reverse transcriptase keratinocyte–hair follicle stem cell interaction exosomes telogen–anagen transition hair cycle regulation |
| title | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
| title_full | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
| title_fullStr | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
| title_full_unstemmed | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
| title_short | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
| title_sort | exosomes derived from fisetin treated keratinocytes mediate hair growth promotion |
| topic | telomerase reverse transcriptase keratinocyte–hair follicle stem cell interaction exosomes telogen–anagen transition hair cycle regulation |
| url | https://www.mdpi.com/2072-6643/13/6/2087 |
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