Evaluating methods and protocols of ferritin-based magnetogenetics
Summary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins. In cells expressing ferritin-tagged TRPV, RF st...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221010622 |
| _version_ | 1818935520398409728 |
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| author | Miriam Hernández-Morales Victor Han Richard H. Kramer Chunlei Liu |
| author_facet | Miriam Hernández-Morales Victor Han Richard H. Kramer Chunlei Liu |
| author_sort | Miriam Hernández-Morales |
| collection | DOAJ |
| description | Summary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins. In cells expressing ferritin-tagged TRPV, RF stimulation increases the cytosolic Ca2+ levels via a biochemical pathway. The interaction between RF and ferritin increases the free cytosolic iron levels that, in turn, trigger chemical reactions producing reactive oxygen species and oxidized lipids that activate the ferritin-tagged TRPV. In this pathway, it is expected that experimental factors that disturb the ferritin expression, the ferritin iron load, the TRPV functional expression, or the cellular redox state will impact the efficiency of RF in activating ferritin-tagged TRPV. Here, we examined several experimental factors that either enhance or abolish the RF control of ferritin-tagged TRPV. The findings may help optimize and establish reproducible magnetogenetic protocols. |
| first_indexed | 2024-12-20T05:21:28Z |
| format | Article |
| id | doaj.art-2bac306fa0e54c2d9737bbd41084dbf1 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-20T05:21:28Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2bac306fa0e54c2d9737bbd41084dbf12022-12-21T19:52:00ZengElsevieriScience2589-00422021-10-012410103094Evaluating methods and protocols of ferritin-based magnetogeneticsMiriam Hernández-Morales0Victor Han1Richard H. Kramer2Chunlei Liu3Department of Electrical Engineering and Computer Sciences, University of California, 505 Cory Hall MC# 1770, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USADepartment of Electrical Engineering and Computer Sciences, University of California, 505 Cory Hall MC# 1770, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USADepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USADepartment of Electrical Engineering and Computer Sciences, University of California, 505 Cory Hall MC# 1770, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA; Corresponding authorSummary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins. In cells expressing ferritin-tagged TRPV, RF stimulation increases the cytosolic Ca2+ levels via a biochemical pathway. The interaction between RF and ferritin increases the free cytosolic iron levels that, in turn, trigger chemical reactions producing reactive oxygen species and oxidized lipids that activate the ferritin-tagged TRPV. In this pathway, it is expected that experimental factors that disturb the ferritin expression, the ferritin iron load, the TRPV functional expression, or the cellular redox state will impact the efficiency of RF in activating ferritin-tagged TRPV. Here, we examined several experimental factors that either enhance or abolish the RF control of ferritin-tagged TRPV. The findings may help optimize and establish reproducible magnetogenetic protocols.http://www.sciencedirect.com/science/article/pii/S2589004221010622BioelectromagneticsGeneticsBiology experimental methods |
| spellingShingle | Miriam Hernández-Morales Victor Han Richard H. Kramer Chunlei Liu Evaluating methods and protocols of ferritin-based magnetogenetics iScience Bioelectromagnetics Genetics Biology experimental methods |
| title | Evaluating methods and protocols of ferritin-based magnetogenetics |
| title_full | Evaluating methods and protocols of ferritin-based magnetogenetics |
| title_fullStr | Evaluating methods and protocols of ferritin-based magnetogenetics |
| title_full_unstemmed | Evaluating methods and protocols of ferritin-based magnetogenetics |
| title_short | Evaluating methods and protocols of ferritin-based magnetogenetics |
| title_sort | evaluating methods and protocols of ferritin based magnetogenetics |
| topic | Bioelectromagnetics Genetics Biology experimental methods |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221010622 |
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