Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to fi...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/8/218 |
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| author | Laura Soumoy Mathilde Wells Ahmad Najem Mohammad Krayem Ghanem Ghanem Stéphanie Hambye Sven Saussez Bertrand Blankert Fabrice Journe |
| author_facet | Laura Soumoy Mathilde Wells Ahmad Najem Mohammad Krayem Ghanem Ghanem Stéphanie Hambye Sven Saussez Bertrand Blankert Fabrice Journe |
| author_sort | Laura Soumoy |
| collection | DOAJ |
| description | Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to find new therapeutic agents to increase patient survival. Toad venoms are a rich source of potentially pharmaceutically active compounds and studies have highlighted their possible effect on cancer cells. We focused on the venoms of two different toad species: <i>Bufo bufo</i> and <i>Rhinella marina</i>. We screened the venom crude extracts, the fractions from crude extracts and isolated biomolecules by studying their antiproliferative properties on melanoma cells aiming to determine the compound or the combination of compounds with the highest antiproliferative effect. Our results indicated strong antiproliferative capacities of toad venoms on melanoma cells. We found that these effects were mainly due to bufadienolides that are cardiotonic steroids potentially acting on the Na<sup>+</sup>/K<sup>+</sup> ATPase pump which is overexpressed in melanoma. Finally, our results indicated that bufalin alone was the most interesting compound among the isolated bufadienolides because it had the highest antiproliferative activity on melanoma cells. |
| first_indexed | 2024-03-10T17:41:31Z |
| format | Article |
| id | doaj.art-2bae868e5a414922aeb9ba518329c47e |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T17:41:31Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-2bae868e5a414922aeb9ba518329c47e2023-11-20T09:41:01ZengMDPI AGBiology2079-77372020-08-019821810.3390/biology9080218Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different VenomsLaura Soumoy0Mathilde Wells1Ahmad Najem2Mohammad Krayem3Ghanem Ghanem4Stéphanie Hambye5Sven Saussez6Bertrand Blankert7Fabrice Journe8Laboratory of Human Anatomy and Experimental Oncology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumLaboratory of Pharmaceutical Analysis, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumLaboratory of Oncology and Experimental Surgery, Institut Jules Bordet (ULB), 1000 Brussels, BelgiumLaboratory of Oncology and Experimental Surgery, Institut Jules Bordet (ULB), 1000 Brussels, BelgiumLaboratory of Oncology and Experimental Surgery, Institut Jules Bordet (ULB), 1000 Brussels, BelgiumLaboratory of Pharmaceutical Analysis, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumLaboratory of Human Anatomy and Experimental Oncology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumLaboratory of Pharmaceutical Analysis, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumLaboratory of Human Anatomy and Experimental Oncology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, BelgiumMelanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to find new therapeutic agents to increase patient survival. Toad venoms are a rich source of potentially pharmaceutically active compounds and studies have highlighted their possible effect on cancer cells. We focused on the venoms of two different toad species: <i>Bufo bufo</i> and <i>Rhinella marina</i>. We screened the venom crude extracts, the fractions from crude extracts and isolated biomolecules by studying their antiproliferative properties on melanoma cells aiming to determine the compound or the combination of compounds with the highest antiproliferative effect. Our results indicated strong antiproliferative capacities of toad venoms on melanoma cells. We found that these effects were mainly due to bufadienolides that are cardiotonic steroids potentially acting on the Na<sup>+</sup>/K<sup>+</sup> ATPase pump which is overexpressed in melanoma. Finally, our results indicated that bufalin alone was the most interesting compound among the isolated bufadienolides because it had the highest antiproliferative activity on melanoma cells.https://www.mdpi.com/2079-7737/9/8/218melanomatargeted therapiesresistance to drugstoad venomcardiotonic steroidssodium pump |
| spellingShingle | Laura Soumoy Mathilde Wells Ahmad Najem Mohammad Krayem Ghanem Ghanem Stéphanie Hambye Sven Saussez Bertrand Blankert Fabrice Journe Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms Biology melanoma targeted therapies resistance to drugs toad venom cardiotonic steroids sodium pump |
| title | Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms |
| title_full | Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms |
| title_fullStr | Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms |
| title_full_unstemmed | Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms |
| title_short | Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms |
| title_sort | toad venom antiproliferative activities on metastatic melanoma bio guided fractionation and screening of the compounds of two different venoms |
| topic | melanoma targeted therapies resistance to drugs toad venom cardiotonic steroids sodium pump |
| url | https://www.mdpi.com/2079-7737/9/8/218 |
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