Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training
We recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant and stress-induced markers. In the present study, we aimed to evalu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-01-01
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| Series: | Redox Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221323171300092X |
| _version_ | 1818752135020412928 |
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| author | Roberta Ceci Maria Reyes Beltran Valls Guglielmo Duranti Ivan Dimauro Federico Quaranta Monica Pittaluga Stefania Sabatini Paolo Caserotti Paolo Parisi Attilio Parisi Daniela Caporossi |
| author_facet | Roberta Ceci Maria Reyes Beltran Valls Guglielmo Duranti Ivan Dimauro Federico Quaranta Monica Pittaluga Stefania Sabatini Paolo Caserotti Paolo Parisi Attilio Parisi Daniela Caporossi |
| author_sort | Roberta Ceci |
| collection | DOAJ |
| description | We recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant and stress-induced markers. In the present study, we aimed to evaluate the impact of EMRT on oxidative stress biomarkers induced in old people (70–75 years) by a single bout of acute, intense exercise. Sixteen subjects randomly assigned to either a control, not exercising group (n=8) or a trained group performing EMRT protocol for 12-weeks (n=8), were submitted to a graded maximal exercise stress test (GXT) at baseline and after the 12-weeks of EMRT protocol, with blood samples collected before, immediately after, 1 and 24 h post-GXT test. Blood glutathione (GSH, GSSG, GSH/GSSG), plasma malonaldehyde (MDA), protein carbonyls and creatine kinase (CK) levels, as well as PBMCs cellular damage (Comet assay, apoptosis) and stress–protein response (Hsp70 and Hsp27 expression) were evaluated. The use of multiple biomarkers allowed us to confirm that EMRT per se neither affected redox homeostasis nor induced any cellular and oxidative damage. Following the GXT, the EMRT group displayed a higher GSH/GSSG ratio and a less pronounced increase in MDA, protein carbonyls and CK levels compared to control group. Moreover, we found that Hsp70 and Hsp27 proteins were induced after GXT only in EMRT group, while any significant modification within 24 h was detected in untrained group. Apoptosis rates and DNA damage did not show any significant variation in relation to EMRT and/or GXT.
In conclusion, the adherence to an EMRT protocol is able to induce a cellular adaptation allowing healthy elderly trained subjects to cope with the oxidative stress induced by an acute exercise more effectively than the aged-matched sedentary subjects. |
| first_indexed | 2024-12-18T04:46:39Z |
| format | Article |
| id | doaj.art-2bb46b0322614d86bb75576287abc02f |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-12-18T04:46:39Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-2bb46b0322614d86bb75576287abc02f2022-12-21T21:20:34ZengElsevierRedox Biology2213-23172014-01-012C657210.1016/j.redox.2013.12.004Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance trainingRoberta Ceci0Maria Reyes Beltran Valls1Guglielmo Duranti2Ivan Dimauro3Federico Quaranta4Monica Pittaluga5Stefania Sabatini6Paolo Caserotti7Paolo Parisi8Attilio Parisi9Daniela Caporossi10Unit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Internal Medicine, Department of Movement, Human and Health Sciences, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyDepartment of Sports Science and Clinical Biomechanics, University of Southern Denmark, Campus vej 55, 5230 Odense M, DenmarkUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Internal Medicine, Department of Movement, Human and Health Sciences, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyUnit of Biology, Genetics and Biochemistry, University of Rome “Foro Italico”, Piazza Lauro De Bosis 15, 00135 Rome, ItalyWe recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant and stress-induced markers. In the present study, we aimed to evaluate the impact of EMRT on oxidative stress biomarkers induced in old people (70–75 years) by a single bout of acute, intense exercise. Sixteen subjects randomly assigned to either a control, not exercising group (n=8) or a trained group performing EMRT protocol for 12-weeks (n=8), were submitted to a graded maximal exercise stress test (GXT) at baseline and after the 12-weeks of EMRT protocol, with blood samples collected before, immediately after, 1 and 24 h post-GXT test. Blood glutathione (GSH, GSSG, GSH/GSSG), plasma malonaldehyde (MDA), protein carbonyls and creatine kinase (CK) levels, as well as PBMCs cellular damage (Comet assay, apoptosis) and stress–protein response (Hsp70 and Hsp27 expression) were evaluated. The use of multiple biomarkers allowed us to confirm that EMRT per se neither affected redox homeostasis nor induced any cellular and oxidative damage. Following the GXT, the EMRT group displayed a higher GSH/GSSG ratio and a less pronounced increase in MDA, protein carbonyls and CK levels compared to control group. Moreover, we found that Hsp70 and Hsp27 proteins were induced after GXT only in EMRT group, while any significant modification within 24 h was detected in untrained group. Apoptosis rates and DNA damage did not show any significant variation in relation to EMRT and/or GXT. In conclusion, the adherence to an EMRT protocol is able to induce a cellular adaptation allowing healthy elderly trained subjects to cope with the oxidative stress induced by an acute exercise more effectively than the aged-matched sedentary subjects.http://www.sciencedirect.com/science/article/pii/S221323171300092XExplosive-type moderate intensity resistance training (EMRT)Graded maximal exercise test (GXT)Oxidative stressHSPsApoptosisElderly |
| spellingShingle | Roberta Ceci Maria Reyes Beltran Valls Guglielmo Duranti Ivan Dimauro Federico Quaranta Monica Pittaluga Stefania Sabatini Paolo Caserotti Paolo Parisi Attilio Parisi Daniela Caporossi Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training Redox Biology Explosive-type moderate intensity resistance training (EMRT) Graded maximal exercise test (GXT) Oxidative stress HSPs Apoptosis Elderly |
| title | Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training |
| title_full | Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training |
| title_fullStr | Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training |
| title_full_unstemmed | Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training |
| title_short | Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training |
| title_sort | oxidative stress responses to a graded maximal exercise test in older adults following explosive type resistance training |
| topic | Explosive-type moderate intensity resistance training (EMRT) Graded maximal exercise test (GXT) Oxidative stress HSPs Apoptosis Elderly |
| url | http://www.sciencedirect.com/science/article/pii/S221323171300092X |
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