Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study
<p>Abstract</p> <p>Background</p> <p>Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird’s Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the reg...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2013-01-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/13/19 |
| _version_ | 1818521121907015680 |
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| author | Chua Kien-Hui Lee Ting-Hun Nagandran Kamini Md Yahaya Nor Hamdan Lee Chew-Tin Tjih Eddie Tan Ti Abdul Aziz Ramlan |
| author_facet | Chua Kien-Hui Lee Ting-Hun Nagandran Kamini Md Yahaya Nor Hamdan Lee Chew-Tin Tjih Eddie Tan Ti Abdul Aziz Ramlan |
| author_sort | Chua Kien-Hui |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird’s Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.</p> <p>Methods</p> <p>A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes’ expression in HACs was measured by real-time PCR. The total amount of prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.</p> <p>Results</p> <p>MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes’ expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.</p> <p>Conclusion</p> <p>Edible Bird’s Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.</p> |
| first_indexed | 2024-12-11T01:46:52Z |
| format | Article |
| id | doaj.art-2bb7cf34092640428021f56fd8ee9486 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-11T01:46:52Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-2bb7cf34092640428021f56fd8ee94862022-12-22T01:24:52ZengBMCBMC Complementary and Alternative Medicine1472-68822013-01-011311910.1186/1472-6882-13-19Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro studyChua Kien-HuiLee Ting-HunNagandran KaminiMd Yahaya Nor HamdanLee Chew-TinTjih Eddie Tan TiAbdul Aziz Ramlan<p>Abstract</p> <p>Background</p> <p>Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird’s Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.</p> <p>Methods</p> <p>A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes’ expression in HACs was measured by real-time PCR. The total amount of prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.</p> <p>Results</p> <p>MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes’ expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.</p> <p>Conclusion</p> <p>Edible Bird’s Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.</p>http://www.biomedcentral.com/1472-6882/13/19 |
| spellingShingle | Chua Kien-Hui Lee Ting-Hun Nagandran Kamini Md Yahaya Nor Hamdan Lee Chew-Tin Tjih Eddie Tan Ti Abdul Aziz Ramlan Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study BMC Complementary and Alternative Medicine |
| title | Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
| title_full | Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
| title_fullStr | Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
| title_full_unstemmed | Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
| title_short | Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
| title_sort | edible bird s nest extract as a chondro protective agent for human chondrocytes isolated from osteoarthritic knee in vitro study |
| url | http://www.biomedcentral.com/1472-6882/13/19 |
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