Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy
The purpose: to explore the clinical effectiveness of a new cell-based surgical method for the treatment of bullous keratopathy that includes intrastromal injection of autologous mononuclear leukocytes into the posterior third of the cornea stroma.Material and methods. The study included 58 patients...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Ophthalmology Publishing Group
2022-10-01
|
| Series: | Oftalʹmologiâ |
| Subjects: | |
| Online Access: | https://www.ophthalmojournal.com/opht/article/view/1924 |
| _version_ | 1797875360844480512 |
|---|---|
| author | M. S. Denisko O. I. Krivosheina E. V. Ivanova |
| author_facet | M. S. Denisko O. I. Krivosheina E. V. Ivanova |
| author_sort | M. S. Denisko |
| collection | DOAJ |
| description | The purpose: to explore the clinical effectiveness of a new cell-based surgical method for the treatment of bullous keratopathy that includes intrastromal injection of autologous mononuclear leukocytes into the posterior third of the cornea stroma.Material and methods. The study included 58 patients with diagnosis of bullous kerathopathy. They were divided into 2 groups — the main group received surgical treatment by the presented method; and the comparison group received a course of conservative therapy.Results. In 10 days the patients in the main group had a normal structure of the cornea epithelium, slight edema of the stroma, thin folds of the Descemet’s membrane and moderate edema of the endothelium. Similar results in the comparison group were achieved in 1 month, and it was the best condition of the cornea. Later corneal edema increased. In 3 months the patients of the main group had a normal structure of corneal epithelium, single thin folds of the Descemet’s membrane, slight edema of the posterior third of the stroma only in the optical zone, and slight edema of the endothelium. The achieved results were preserved for 12 months. The best correct visual acuity (BCVA) in the main group was achieved by the 6th month. It increased 10 times from the initial level, and the corneal thickness decreased by 27 % from the initial level by the 9th month. In the comparison group, the BCVA was achieved by the 1st month. It increased 2.7 times from the initial level; the maximum decrease in the index of pachymetry by 6.7 % was achieved by the 3rd month from the initial level, and then these parameters increased.Conclusion. The using of a surgical method for the treatment of bullous keratopathy, including intrastromal injection of suspension of autologous mononuclear leukocytes, contributes to the relief of corneal syndrome, increases the transparency of the cornea and restores its normal thickness. These changes are accompanied by increase of BCVA in the postoperative period compared to the course of conservative therapy. |
| first_indexed | 2024-04-10T01:46:26Z |
| format | Article |
| id | doaj.art-2bba08c7cf1143d9903c48b3482513d5 |
| institution | Directory Open Access Journal |
| issn | 1816-5095 2500-0845 |
| language | Russian |
| last_indexed | 2024-04-10T01:46:26Z |
| publishDate | 2022-10-01 |
| publisher | Ophthalmology Publishing Group |
| record_format | Article |
| series | Oftalʹmologiâ |
| spelling | doaj.art-2bba08c7cf1143d9903c48b3482513d52023-03-13T09:08:43ZrusOphthalmology Publishing GroupOftalʹmologiâ1816-50952500-08452022-10-0119357157710.18008/1816-5095-2022-3-571-577944Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous KeratopathyM. S. Denisko0O. I. Krivosheina1E. V. Ivanova2ФГБОУ ВО «Сибирский государственный медицинский университет» Министерства здравоохранения Российской Федерации; ООО «Гранд Ретина»ФГБОУ ВО «Сибирский государственный медицинский университет» Министерства здравоохранения Российской Федерации; ООО «Гранд Ретина»ФГБОУ ВО «Сибирский государственный медицинский университет» Министерства здравоохранения Российской Федерации; ООО «Гранд Ретина»The purpose: to explore the clinical effectiveness of a new cell-based surgical method for the treatment of bullous keratopathy that includes intrastromal injection of autologous mononuclear leukocytes into the posterior third of the cornea stroma.Material and methods. The study included 58 patients with diagnosis of bullous kerathopathy. They were divided into 2 groups — the main group received surgical treatment by the presented method; and the comparison group received a course of conservative therapy.Results. In 10 days the patients in the main group had a normal structure of the cornea epithelium, slight edema of the stroma, thin folds of the Descemet’s membrane and moderate edema of the endothelium. Similar results in the comparison group were achieved in 1 month, and it was the best condition of the cornea. Later corneal edema increased. In 3 months the patients of the main group had a normal structure of corneal epithelium, single thin folds of the Descemet’s membrane, slight edema of the posterior third of the stroma only in the optical zone, and slight edema of the endothelium. The achieved results were preserved for 12 months. The best correct visual acuity (BCVA) in the main group was achieved by the 6th month. It increased 10 times from the initial level, and the corneal thickness decreased by 27 % from the initial level by the 9th month. In the comparison group, the BCVA was achieved by the 1st month. It increased 2.7 times from the initial level; the maximum decrease in the index of pachymetry by 6.7 % was achieved by the 3rd month from the initial level, and then these parameters increased.Conclusion. The using of a surgical method for the treatment of bullous keratopathy, including intrastromal injection of suspension of autologous mononuclear leukocytes, contributes to the relief of corneal syndrome, increases the transparency of the cornea and restores its normal thickness. These changes are accompanied by increase of BCVA in the postoperative period compared to the course of conservative therapy.https://www.ophthalmojournal.com/opht/article/view/1924эндотелиально-эпителиальная дистрофия роговицыаутологичные мононуклеарные лейкоциты кровицитокинырепаративная регенерация |
| spellingShingle | M. S. Denisko O. I. Krivosheina E. V. Ivanova Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy Oftalʹmologiâ эндотелиально-эпителиальная дистрофия роговицы аутологичные мононуклеарные лейкоциты крови цитокины репаративная регенерация |
| title | Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy |
| title_full | Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy |
| title_fullStr | Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy |
| title_full_unstemmed | Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy |
| title_short | Clinical and Functional Results of the Use of Suspension of Autologous Mononuclear Leucocytes in the Surgical Treatment of Bullous Keratopathy |
| title_sort | clinical and functional results of the use of suspension of autologous mononuclear leucocytes in the surgical treatment of bullous keratopathy |
| topic | эндотелиально-эпителиальная дистрофия роговицы аутологичные мононуклеарные лейкоциты крови цитокины репаративная регенерация |
| url | https://www.ophthalmojournal.com/opht/article/view/1924 |
| work_keys_str_mv | AT msdenisko clinicalandfunctionalresultsoftheuseofsuspensionofautologousmononuclearleucocytesinthesurgicaltreatmentofbullouskeratopathy AT oikrivosheina clinicalandfunctionalresultsoftheuseofsuspensionofautologousmononuclearleucocytesinthesurgicaltreatmentofbullouskeratopathy AT evivanova clinicalandfunctionalresultsoftheuseofsuspensionofautologousmononuclearleucocytesinthesurgicaltreatmentofbullouskeratopathy |