Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples

Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared an...

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Main Authors: Lianrun Huang, Fuxuan Zhang, Fangxuan Li, Yin Jia, Minghua Wang, Xiude Hua, Limin Wang
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Biosensors
Subjects:
Online Access:https://www.mdpi.com/2079-6374/12/11/1058
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author Lianrun Huang
Fuxuan Zhang
Fangxuan Li
Yin Jia
Minghua Wang
Xiude Hua
Limin Wang
author_facet Lianrun Huang
Fuxuan Zhang
Fangxuan Li
Yin Jia
Minghua Wang
Xiude Hua
Limin Wang
author_sort Lianrun Huang
collection DOAJ
description Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.
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spelling doaj.art-2bbcc2356f1049349ee925e22f04ae4d2023-11-24T07:49:14ZengMDPI AGBiosensors2079-63742022-11-011211105810.3390/bios12111058Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural SamplesLianrun Huang0Fuxuan Zhang1Fangxuan Li2Yin Jia3Minghua Wang4Xiude Hua5Limin Wang6College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.https://www.mdpi.com/2079-6374/12/11/1058cypermethrinmonoclonal antibodycolloidal goldlateral flow immunoassay
spellingShingle Lianrun Huang
Fuxuan Zhang
Fangxuan Li
Yin Jia
Minghua Wang
Xiude Hua
Limin Wang
Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
Biosensors
cypermethrin
monoclonal antibody
colloidal gold
lateral flow immunoassay
title Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
title_full Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
title_fullStr Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
title_full_unstemmed Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
title_short Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
title_sort development of ic elisa and colloidal gold lateral flow immunoassay for the determination of cypermethrin in agricultural samples
topic cypermethrin
monoclonal antibody
colloidal gold
lateral flow immunoassay
url https://www.mdpi.com/2079-6374/12/11/1058
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