Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples
Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared an...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-11-01
|
Series: | Biosensors |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-6374/12/11/1058 |
_version_ | 1797465760724942848 |
---|---|
author | Lianrun Huang Fuxuan Zhang Fangxuan Li Yin Jia Minghua Wang Xiude Hua Limin Wang |
author_facet | Lianrun Huang Fuxuan Zhang Fangxuan Li Yin Jia Minghua Wang Xiude Hua Limin Wang |
author_sort | Lianrun Huang |
collection | DOAJ |
description | Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products. |
first_indexed | 2024-03-09T18:27:07Z |
format | Article |
id | doaj.art-2bbcc2356f1049349ee925e22f04ae4d |
institution | Directory Open Access Journal |
issn | 2079-6374 |
language | English |
last_indexed | 2024-03-09T18:27:07Z |
publishDate | 2022-11-01 |
publisher | MDPI AG |
record_format | Article |
series | Biosensors |
spelling | doaj.art-2bbcc2356f1049349ee925e22f04ae4d2023-11-24T07:49:14ZengMDPI AGBiosensors2079-63742022-11-011211105810.3390/bios12111058Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural SamplesLianrun Huang0Fuxuan Zhang1Fangxuan Li2Yin Jia3Minghua Wang4Xiude Hua5Limin Wang6College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.https://www.mdpi.com/2079-6374/12/11/1058cypermethrinmonoclonal antibodycolloidal goldlateral flow immunoassay |
spellingShingle | Lianrun Huang Fuxuan Zhang Fangxuan Li Yin Jia Minghua Wang Xiude Hua Limin Wang Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples Biosensors cypermethrin monoclonal antibody colloidal gold lateral flow immunoassay |
title | Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples |
title_full | Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples |
title_fullStr | Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples |
title_full_unstemmed | Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples |
title_short | Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples |
title_sort | development of ic elisa and colloidal gold lateral flow immunoassay for the determination of cypermethrin in agricultural samples |
topic | cypermethrin monoclonal antibody colloidal gold lateral flow immunoassay |
url | https://www.mdpi.com/2079-6374/12/11/1058 |
work_keys_str_mv | AT lianrunhuang developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT fuxuanzhang developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT fangxuanli developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT yinjia developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT minghuawang developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT xiudehua developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples AT liminwang developmentoficelisaandcolloidalgoldlateralflowimmunoassayforthedeterminationofcypermethrininagriculturalsamples |