Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression
<p>Abstract</p> <p>In this study, a full-length cDNA of the porcine <it>ENO3 </it>gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | deu |
| Published: |
BMC
2008-09-01
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| Series: | Genetics Selection Evolution |
| Subjects: | |
| Online Access: | http://www.gsejournal.org/content/40/5/563 |
| Summary: | <p>Abstract</p> <p>In this study, a full-length cDNA of the porcine <it>ENO3 </it>gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of <it>ENO3 </it>is highly expressed in liver and lung, while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of <it>ENO3 </it>is different between Yorkshire and Meishan pig breeds. Furthermore, real-time polymerase chain reaction revealed that, in Yorkshire pigs, skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover, expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2, with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the <it>ENO3 </it>gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage, average backfat thickness, meat marbling and intramuscular fat in two different populations.</p> |
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| ISSN: | 0999-193X 1297-9686 |