Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression
<p>Abstract</p> <p>In this study, a full-length cDNA of the porcine <it>ENO3 </it>gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | deu |
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BMC
2008-09-01
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| Series: | Genetics Selection Evolution |
| Subjects: | |
| Online Access: | http://www.gsejournal.org/content/40/5/563 |
| _version_ | 1818148143540207616 |
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| author | Xiong Yuanzhu Long Liangqi Wu Xiaoxiong Zhou Donghai Deng Changyan Wu Jian |
| author_facet | Xiong Yuanzhu Long Liangqi Wu Xiaoxiong Zhou Donghai Deng Changyan Wu Jian |
| author_sort | Xiong Yuanzhu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>In this study, a full-length cDNA of the porcine <it>ENO3 </it>gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of <it>ENO3 </it>is highly expressed in liver and lung, while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of <it>ENO3 </it>is different between Yorkshire and Meishan pig breeds. Furthermore, real-time polymerase chain reaction revealed that, in Yorkshire pigs, skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover, expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2, with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the <it>ENO3 </it>gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage, average backfat thickness, meat marbling and intramuscular fat in two different populations.</p> |
| first_indexed | 2024-12-11T12:46:27Z |
| format | Article |
| id | doaj.art-2bbf4c2564c24264b902243cfba290b2 |
| institution | Directory Open Access Journal |
| issn | 0999-193X 1297-9686 |
| language | deu |
| last_indexed | 2024-12-11T12:46:27Z |
| publishDate | 2008-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Genetics Selection Evolution |
| spelling | doaj.art-2bbf4c2564c24264b902243cfba290b22022-12-22T01:06:48ZdeuBMCGenetics Selection Evolution0999-193X1297-96862008-09-0140556357910.1186/1297-9686-40-5-563Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expressionXiong YuanzhuLong LiangqiWu XiaoxiongZhou DonghaiDeng ChangyanWu Jian<p>Abstract</p> <p>In this study, a full-length cDNA of the porcine <it>ENO3 </it>gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of <it>ENO3 </it>is highly expressed in liver and lung, while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of <it>ENO3 </it>is different between Yorkshire and Meishan pig breeds. Furthermore, real-time polymerase chain reaction revealed that, in Yorkshire pigs, skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover, expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2, with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the <it>ENO3 </it>gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage, average backfat thickness, meat marbling and intramuscular fat in two different populations.</p>http://www.gsejournal.org/content/40/5/563pig<it>ENO3</it>polymorphism |
| spellingShingle | Xiong Yuanzhu Long Liangqi Wu Xiaoxiong Zhou Donghai Deng Changyan Wu Jian Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression Genetics Selection Evolution pig <it>ENO3</it> polymorphism |
| title | Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression |
| title_full | Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression |
| title_fullStr | Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression |
| title_full_unstemmed | Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression |
| title_short | Characterization of porcine <it>ENO3</it>: genomic and cDNA structure, polymorphism and expression |
| title_sort | characterization of porcine it eno3 it genomic and cdna structure polymorphism and expression |
| topic | pig <it>ENO3</it> polymorphism |
| url | http://www.gsejournal.org/content/40/5/563 |
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