| Summary: | Gram-negative, rod-shaped, and aerobic bacteria designated chi1<sup>T</sup> and chi5<sup>T</sup> were isolated from the root of <i>Suaeda japonica</i> Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1<sup>T</sup> and chi5<sup>T</sup> confirmed that they were related to the genera <i>Marinobacter</i> and <i>Wenyingzhuangia</i>, respectively. For the novel strains chi1<sup>T</sup> and chi5<sup>T</sup>, the digital DNA–DNA hybridization values (19–20% and 22.1–36.6%, respectively) and average nucleotide identity values (74.4–76.5% and 79.1–88.9%, respectively) fell within the range for the genera <i>Marinobacter</i> and <i>Wenyingzhuangia</i>, respectively. Pangenome analyses of the novel strains chi1<sup>T</sup> and chi5<sup>T</sup> revealed 357 and 368 singletons genes, respectively. The genomic DNA G + C contents of the strains chi1<sup>T</sup> and chi5<sup>T</sup> were 57.2% and 31.5%, respectively. The major fatty acids of strain chi1<sup>T</sup> were C<sub>12:0</sub>, C<sub>16:0</sub>, and summed feature 3 (C<sub>16:1</sub> <i>ω</i>6<i>c</i> and/or C<sub>16:1</sub><i>ω</i>7<i>c</i>), while those of the strain chi5<sup>T</sup> were iso-C<sub>15:0</sub> 3OH, iso-C<sub>17:0</sub> 3OH, and iso-C<sub>15:0</sub>. Data from the phylogenetic, phylogenomic, pangenome, genomic, physiological, and biochemical analyses indicated that the novel strains were distinct. Therefore, we propose the names <i>Marinobacter suadae</i> (type strain chi1<sup>T</sup> = KACC 23259<sup>T</sup> = TBRC 17652<sup>T</sup>) and <i>Wenyingzhangia gilva</i> (type strain chi5<sup>T</sup> = KACC 23262<sup>T</sup> = TBRC 17900<sup>T</sup>) for the studied bacterial strains.
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