Optimal detection protocol of Tetracapsuloides bryosalmonae by environmental DNA: A comparison of qPCR and ddPCR approaches

Optimal detection protocol of Tetracapsuloides bryosalmonae by environmental DNA: A comparison of qPCR and ddPCR approaches

AbstractInvestigation of environmental DNA (eDNA) is increasingly used to precisely and non‐invasively detect and monitor pathogens. Among these, Tetracapsuloides bryosalmonae is a myxozoan endoparasite that causes proliferative kidney disease (PKD) in salmonid fish. Although the detection of T. bry...

Full description

Bibliographic Details
Main Authors:	Moritz Stelzer, James Ord, Sabrina Neyrinck, Jonas Steiner, Hanna Hartikainen, Rein Brys, Heike Schmidt‐Posthaus
Format:	Article
Language:	English
Published:	Wiley 2024-01-01
Series:	Environmental DNA
Subjects:	environmental DNA method comparison monitoring protocol non‐invasive monitoring proliferative kidney disease Tetracapsuloides bryosalmonae
Online Access:	https://doi.org/10.1002/edn3.501

Similar Items

Use of environmental DNA to detect the myxozoan endoparasite Tetracapsuloides bryosalmonae in large Norwegian lakes
by: Tone Jøran Oredalen, et al.
Published: (2022-11-01)

Identification of in vivo induced antigens of the malacosporean parasite Tetracapsuloides bryosalmonae (Cnidaria) using in vivo induced antigen technology
by: Gokhlesh Kumar, et al.
Published: (2022-10-01)

Persistence, impacts and environmental drivers of covert infections in invertebrate hosts
by: Inês Fontes, et al.
Published: (2017-11-01)

Keeping an Eye on Wild Brown Trout (Salmo trutta) Populations: Correlation Between Temperature, Environmental Parameters, and Proliferative Kidney Disease
by: Aurélie Rubin, et al.
Published: (2019-08-01)

Dysregulation of B Cell Activity During Proliferative Kidney Disease in Rainbow Trout
by: Beatriz Abos, et al.
Published: (2018-05-01)

Transcriptome profiling of posterior kidney of brown trout, Salmo trutta, during proliferative kidney disease
by: Arun Sudhagar, et al.
Published: (2019-11-01)

Genome-wide alternative splicing profile in the posterior kidney of brown trout (Salmo trutta) during proliferative kidney disease
by: Arun Sudhagar, et al.
Published: (2022-06-01)

Quantifying the Detection Sensitivity and Precision of qPCR and ddPCR Mechanisms for eDNA Samples
by: Gledis Guri, et al.
Published: (2024-12-01)

Cranial Mandibular Fibrosis Syndrome in Adult Farmed Rainbow Trout <i>Oncorhynchus mykiss</i>
by: Irene Cano, et al.
Published: (2021-04-01)

Proliferative Kidney Disease and Proliferative Darkening Syndrome are Linked with Brown Trout (<i>Salmo trutta fario</i>) Mortalities in the Pre-Alpine Isar River
by: Daniela Arndt, et al.
Published: (2019-10-01)

Reliable Field Assessment of Proliferative Kidney Disease in Wild Brown Trout, <i>Salmo trutta</i>, Populations: When Is the Optimal Sampling Period?
by: Aurélie Rubin, et al.
Published: (2022-06-01)

Data of de novo transcriptome assembly of the myxozoan parasite Tetracapsuloides bryosalmonae
by: Gokhlesh Kumar, et al.
Published: (2021-04-01)

Effects of preservation strategies on environmental DNA detection and quantification using ddPCR
by: Quentin Mauvisseau, et al.
Published: (2021-07-01)

Thermotactic behaviour in lacustrine and riverine forms of Salmo trutta and its relevance to an emerging parasitic disease (PKD) in the wake of climate change
by: Albert Ros, et al.
Published: (2024-06-01)

The impact of Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections on pathology in rainbow trout
by: Mohamed H. Kotob, et al.
Published: (2017-09-01)

The Malacosporean Myxozoan Parasite <i>Tetracapsuloides bryosalmonae</i>: A Threat to Wild Salmonids
by: Arun Sudhagar, et al.
Published: (2019-12-01)

Seems fishy: environmental DNA impacts on sketa22 quality control in salmonidae dominated waterbodies using qPCR and ddPCR
by: John J Hart, et al.
Published: (2023-01-01)

Accurate detection and quantification of seasonal abundance of American bullfrog (Lithobates catesbeianus) using ddPCR eDNA assays
by: Teun Everts, et al.
Published: (2021-05-01)

A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision
by: Yu Wang, et al.
Published: (2020-12-01)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
by: Changwoo Park, et al.
Published: (2023-02-01)

The myxozoans Myxobolus cerebralis and Tetracapsuloides bryosalmonae modulate rainbow trout immune responses: quantitative shotgun proteomics at the portals of entry after single and co-infections
by: Mona Saleh, et al.
Published: (2024-05-01)

Back From the Brink: Alterations in B and T Cell Responses Modulate Recovery of Rainbow Trout From Chronic Immunopathological Tetracapsuloides bryosalmonae Infection
by: Christyn Bailey, et al.
Published: (2020-06-01)

Selection of reference genes for qPCR- and ddPCR-based analyses of gene expression in Senescing Barley leaves.
by: Agnieszka Zmienko, et al.
Published: (2015-01-01)

Comparison of qPCR with ddPCR for the Quantification of JC Polyomavirus in CSF from Patients with Progressive Multifocal Leukoencephalopathy
by: Nyater Ngouth, et al.
Published: (2022-06-01)

Red blood cells in proliferative kidney disease—rainbow trout (Oncorhynchus mykiss) infected by Tetracapsuloides bryosalmonae harbor IgM+ red blood cells
by: Justin T. H. Chan, et al.
Published: (2023-02-01)

Differential modulation of host immune genes in the kidney and cranium of the rainbow trout (Oncorhynchus mykiss) in response to Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections
by: Mohamed H. Kotob, et al.
Published: (2018-05-01)

Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
by: Yanfang Huang, et al.
Published: (2021-08-01)

ddPCR for the Detection and Absolute Quantification of Oropouche Virus
by: Elena Pomari, et al.
Published: (2024-09-01)

Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals
by: María-Ignacia Meza Cerda, et al.
Published: (2020-11-01)

Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays.
by: Samuel Long, et al.
Published: (2020-01-01)

Somatic Mutation Allelic Ratio Test Using ddPCR (SMART-ddPCR): An Accurate Method for Assessment of Preferential Allelic Imbalance in Tumor DNA.
by: Adam J de Smith, et al.
Published: (2015-01-01)

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution
by: Laura A. E. Van Poelvoorde, et al.
Published: (2021-11-01)

Detecting endangered pinto abalone (Haliotis kamtschatkana) using environmental DNA: Comparison of ddPCR, qPCR, and conventional diver surveys
by: James L. Dimond, et al.
Published: (2022-11-01)

Evaluating ddPCR and RT-qPCR methods for PIK3CA mutation detection in head and neck cancer via precision oncology
by: Akankshya Dash, et al.
Published: (2024-09-01)

Comparison of RT-ddPCR and RT-qPCR platforms for SARS-CoV-2 detection: Implications for future outbreaks of infectious diseases
by: Jiahui Ding, et al.
Published: (2024-01-01)

Influence of distance from source population and seasonality in eDNA detection of white‐clawed crayfish, through qPCR and ddPCR assays
by: Thomas Baudry, et al.
Published: (2023-07-01)

Differentially expressed transcripts of Tetracapsuloides bryosalmonae (Cnidaria) between carrier and dead-end hosts involved in key biological processes: novel insights from a coupled approach of FACS and RNA sequencing
by: Saloni Shivam, et al.
Published: (2023-06-01)

Applying improved ddPCR to reliable quantification of MPXV in clinical settings
by: Chudan Liang, et al.
Published: (2024-07-01)

HIV reservoir quantification using cross-subtype multiplex ddPCR
by: Noah A.J. Cassidy, et al.
Published: (2022-01-01)

First transcriptome analysis of bryozoan Fredericella sultana, the primary host of myxozoan parasite Tetracapsuloides bryosalmonae
by: Gokhlesh Kumar, et al.
Published: (2020-04-01)