Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant
Abstract Crocus sativus is the only plant species which produces apocarotenoids like crocin, picrocrocin and safranal in significant amounts. These compounds impart organoleptic properties to saffron (dried stigmas of Crocus flower) making it world’s costliest spice. Crocus apocarotenoids have treme...
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| Format: | Article |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08974-z |
| _version_ | 1818425252265328640 |
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| author | Zahoor Ahmed Wani Amit Kumar Phalisteen Sultan Kushal Bindu Syed Riyaz-Ul-Hassan Nasheeman Ashraf |
| author_facet | Zahoor Ahmed Wani Amit Kumar Phalisteen Sultan Kushal Bindu Syed Riyaz-Ul-Hassan Nasheeman Ashraf |
| author_sort | Zahoor Ahmed Wani |
| collection | DOAJ |
| description | Abstract Crocus sativus is the only plant species which produces apocarotenoids like crocin, picrocrocin and safranal in significant amounts. These compounds impart organoleptic properties to saffron (dried stigmas of Crocus flower) making it world’s costliest spice. Crocus apocarotenoids have tremendous medicinal properties as well. Effect of endophytes on Crocus apocarotenoid production and the molecular mechanism involved has not been reported so far. Here we studied the effect of an oleaginous fungal endophyte, Mortierella alpina CS10E4 on Crocus growth, apocarotenoid metabolism and tolerance to corm rot disease. The results demonstrated that there was a significant improvement in many morphological and physiological traits in endophyte treated Crocus plants including total biomass and size of corms, stigma biomass, number of apical sprouting buds, and number of adventitious roots. The endophyte also shifted metabolic flux towards enhanced production of apocarotenoids by modulating the expression of key pathway genes. Further, M. alpina CS10E4 enhanced tolerance to corm rot disease by releasing arachidonic acid which acts as conserved defense signal and induces jasmonic acid production in endophyte treated Crocus corms. This is first report on effect of a fungal endophyte on Crocus apocarotenoid metabolism and stress tolerance. |
| first_indexed | 2024-12-14T14:10:59Z |
| format | Article |
| id | doaj.art-2bc1d7e393994594ae957500423e30db |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:10:59Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2bc1d7e393994594ae957500423e30db2022-12-21T22:58:18ZengNature PortfolioScientific Reports2045-23222017-08-017111110.1038/s41598-017-08974-zMortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plantZahoor Ahmed Wani0Amit Kumar1Phalisteen Sultan2Kushal Bindu3Syed Riyaz-Ul-Hassan4Nasheeman Ashraf5Plant Biotechnology Division, CSIR-Indian Institute of Integrative MedicineInstrumentation Division, CSIR-Indian Institute of Integrative Medicine, Canal RoadPlant Biotechnology Division, CSIR-Indian Institute of Integrative MedicineInstrumentation Division, CSIR-Indian Institute of Integrative Medicine, Canal RoadAcademy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative MedicinePlant Biotechnology Division, CSIR-Indian Institute of Integrative MedicineAbstract Crocus sativus is the only plant species which produces apocarotenoids like crocin, picrocrocin and safranal in significant amounts. These compounds impart organoleptic properties to saffron (dried stigmas of Crocus flower) making it world’s costliest spice. Crocus apocarotenoids have tremendous medicinal properties as well. Effect of endophytes on Crocus apocarotenoid production and the molecular mechanism involved has not been reported so far. Here we studied the effect of an oleaginous fungal endophyte, Mortierella alpina CS10E4 on Crocus growth, apocarotenoid metabolism and tolerance to corm rot disease. The results demonstrated that there was a significant improvement in many morphological and physiological traits in endophyte treated Crocus plants including total biomass and size of corms, stigma biomass, number of apical sprouting buds, and number of adventitious roots. The endophyte also shifted metabolic flux towards enhanced production of apocarotenoids by modulating the expression of key pathway genes. Further, M. alpina CS10E4 enhanced tolerance to corm rot disease by releasing arachidonic acid which acts as conserved defense signal and induces jasmonic acid production in endophyte treated Crocus corms. This is first report on effect of a fungal endophyte on Crocus apocarotenoid metabolism and stress tolerance.https://doi.org/10.1038/s41598-017-08974-z |
| spellingShingle | Zahoor Ahmed Wani Amit Kumar Phalisteen Sultan Kushal Bindu Syed Riyaz-Ul-Hassan Nasheeman Ashraf Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant Scientific Reports |
| title | Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| title_full | Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| title_fullStr | Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| title_full_unstemmed | Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| title_short | Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| title_sort | mortierella alpina cs10e4 an oleaginous fungal endophyte of crocus sativus l enhances apocarotenoid biosynthesis and stress tolerance in the host plant |
| url | https://doi.org/10.1038/s41598-017-08974-z |
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