DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71
Objectives: To explore the mechanisms of interferon-induced transmembrane protein 3 (IFITM3) in response to enterovirus-71-associated hand, foot and mouth disease (EV71-HFMD), in terms of DNA methylation, single-nucleotide polymorphism (SNP) genotype and gene expression. Methods: In total, 120 patie...
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| Format: | Article |
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Elsevier
2021-04-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221001351 |
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| author | Mei Li Ya-Ping Li Hui-Ling Deng Mu-Qi Wang Yuan Chen Yu-Feng Zhang Jun Wang Shuang-Suo Dang |
| author_facet | Mei Li Ya-Ping Li Hui-Ling Deng Mu-Qi Wang Yuan Chen Yu-Feng Zhang Jun Wang Shuang-Suo Dang |
| author_sort | Mei Li |
| collection | DOAJ |
| description | Objectives: To explore the mechanisms of interferon-induced transmembrane protein 3 (IFITM3) in response to enterovirus-71-associated hand, foot and mouth disease (EV71-HFMD), in terms of DNA methylation, single-nucleotide polymorphism (SNP) genotype and gene expression. Methods: In total, 120 patients with EV71-HFMD (60 with mild EV71-HFMD and 60 with severe EV71-HFMD) and 60 healthy controls were enrolled in this study. SNP genotype, IFITM3 promoter methylation and mRNA expression of peripheral blood mononuclear cells were examined using the improved multi-temperature ligase detection reaction, quantitative reverse transcriptase polymerase chain reaction and MiSeq, respectively. Results: The distribution of methylation in patients with EV71-HFMD was significantly lower compared with healthy controls, and the severe EV71-HFMD group showed the lowest frequency of IFITM3 promoter methylation. The average level of IFITM3 promoter CpG methylation was negatively correlated with IFITM3 mRNA expression, and hypermethylation of several specific CpG units contributed to IFITM3 downregulation. IFITM3 expression and promoter methylation correlated with EV71 infection progression, especially in the severe EV71-HFMD group. Compared with mild cases, genotype GG and the G allele of rs12252 were over-represented in patients with severe EV71-HFMD. Conclusions: IFITM3 methylation status and SNP genotyping may help clinicians to choose the correct treatment strategy for patients with EV71-HFMD. |
| first_indexed | 2024-12-17T08:31:06Z |
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| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-17T08:31:06Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
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| series | International Journal of Infectious Diseases |
| spelling | doaj.art-2bc46057e1c84a078d4853ea2945e61c2022-12-21T21:56:36ZengElsevierInternational Journal of Infectious Diseases1201-97122021-04-01105199208DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71Mei Li0Ya-Ping Li1Hui-Ling Deng2Mu-Qi Wang3Yuan Chen4Yu-Feng Zhang5Jun Wang6Shuang-Suo Dang7Department of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China; Corresponding author at: Department of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, 710004, China.Department of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China; Department of Infectious Diseases, Xi'an Children's Hospital, Xi'an, China; Department of Paediatrics, Xi'an Central Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Children's Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Children's Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Children's Hospital, Xi'an, ChinaDepartment of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, ChinaObjectives: To explore the mechanisms of interferon-induced transmembrane protein 3 (IFITM3) in response to enterovirus-71-associated hand, foot and mouth disease (EV71-HFMD), in terms of DNA methylation, single-nucleotide polymorphism (SNP) genotype and gene expression. Methods: In total, 120 patients with EV71-HFMD (60 with mild EV71-HFMD and 60 with severe EV71-HFMD) and 60 healthy controls were enrolled in this study. SNP genotype, IFITM3 promoter methylation and mRNA expression of peripheral blood mononuclear cells were examined using the improved multi-temperature ligase detection reaction, quantitative reverse transcriptase polymerase chain reaction and MiSeq, respectively. Results: The distribution of methylation in patients with EV71-HFMD was significantly lower compared with healthy controls, and the severe EV71-HFMD group showed the lowest frequency of IFITM3 promoter methylation. The average level of IFITM3 promoter CpG methylation was negatively correlated with IFITM3 mRNA expression, and hypermethylation of several specific CpG units contributed to IFITM3 downregulation. IFITM3 expression and promoter methylation correlated with EV71 infection progression, especially in the severe EV71-HFMD group. Compared with mild cases, genotype GG and the G allele of rs12252 were over-represented in patients with severe EV71-HFMD. Conclusions: IFITM3 methylation status and SNP genotyping may help clinicians to choose the correct treatment strategy for patients with EV71-HFMD.http://www.sciencedirect.com/science/article/pii/S1201971221001351IFITM3DNA methylationHand, foot and mouth diseaseEnterovirus 71Single-nucleotide polymorphisms |
| spellingShingle | Mei Li Ya-Ping Li Hui-Ling Deng Mu-Qi Wang Yuan Chen Yu-Feng Zhang Jun Wang Shuang-Suo Dang DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 International Journal of Infectious Diseases IFITM3 DNA methylation Hand, foot and mouth disease Enterovirus 71 Single-nucleotide polymorphisms |
| title | DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 |
| title_full | DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 |
| title_fullStr | DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 |
| title_full_unstemmed | DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 |
| title_short | DNA methylation and SNP in IFITM3 are correlated with hand, foot and mouth disease caused by enterovirus 71 |
| title_sort | dna methylation and snp in ifitm3 are correlated with hand foot and mouth disease caused by enterovirus 71 |
| topic | IFITM3 DNA methylation Hand, foot and mouth disease Enterovirus 71 Single-nucleotide polymorphisms |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221001351 |
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