Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek
Root rot is one of the most significant soil and seed-borne fungal diseases, limiting the cultivation of fenugreek plants. Endophytic bacteria and their natural bioproducts have emerged as growth promoters and disease suppressors in the current era. Despite limited research, seeds are a good funder...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/17/5546 |
| _version_ | 1797494099842957312 |
|---|---|
| author | Ehsan M. Rashad Dalia M. Shaheen Abdulaziz A. Al-Askar Khalid M. Ghoneem Amr Abker Arishi El Sayed A. Hassan WesamEldin I. A. Saber |
| author_facet | Ehsan M. Rashad Dalia M. Shaheen Abdulaziz A. Al-Askar Khalid M. Ghoneem Amr Abker Arishi El Sayed A. Hassan WesamEldin I. A. Saber |
| author_sort | Ehsan M. Rashad |
| collection | DOAJ |
| description | Root rot is one of the most significant soil and seed-borne fungal diseases, limiting the cultivation of fenugreek plants. Endophytic bacteria and their natural bioproducts have emerged as growth promoters and disease suppressors in the current era. Despite limited research, seeds are a good funder of endophytic microbiomes, which are transmitted from them to other seedling parts, thereby providing a shield against biotic and abiotic anxiety and promoting the growth at early germination and later stages. The current study evaluated the hypothesis that seed endophytic bacteria and their lytic enzymes, growth promotors, and antifungal molecules can induce growth, and inhibit root rot disease development at the same time. The isolation trial from fenugreek seeds revealed a lytic <i>Achromobacter</i> sp., which produces indole acetic acid, has antifungal compounds (e.g., 2-Butanol, 3,3’-oxybis-), and reduces the growth of <i>Rhizoctonia solani</i> by 43.75%. Under the greenhouse and natural field conditions, bacterial cells and/or supernatant improved the growth, physiology, and yield performance of fenugreek plants, and effectively suppressed the progress of root rot disease; this is the first extensive study that uses a new seed-borne endophytic bacterium as a plant-growth-promoting, and biocontrol tool against the sclerotia-forming; <i>R. solani</i>; the causative of fenugreek root rot. |
| first_indexed | 2024-03-10T01:29:27Z |
| format | Article |
| id | doaj.art-2bc7aa2cc79b4bcfb68d947e4a0a3f7c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:29:27Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2bc7aa2cc79b4bcfb68d947e4a0a3f7c2023-11-23T13:43:51ZengMDPI AGMolecules1420-30492022-08-012717554610.3390/molecules27175546Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of FenugreekEhsan M. Rashad0Dalia M. Shaheen1Abdulaziz A. Al-Askar2Khalid M. Ghoneem3Amr Abker Arishi4El Sayed A. Hassan5WesamEldin I. A. Saber6Seed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptSeed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptBotany and Microbiology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi ArabiaSeed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptSchool of Molecular Sciences, The University of Western Australia, Perth, WA 6009, AustraliaSeed Technology Research Department, Field Crops Research Institute, Agricultural Research Center, Giza 12619, EgyptMicrobial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, EgyptRoot rot is one of the most significant soil and seed-borne fungal diseases, limiting the cultivation of fenugreek plants. Endophytic bacteria and their natural bioproducts have emerged as growth promoters and disease suppressors in the current era. Despite limited research, seeds are a good funder of endophytic microbiomes, which are transmitted from them to other seedling parts, thereby providing a shield against biotic and abiotic anxiety and promoting the growth at early germination and later stages. The current study evaluated the hypothesis that seed endophytic bacteria and their lytic enzymes, growth promotors, and antifungal molecules can induce growth, and inhibit root rot disease development at the same time. The isolation trial from fenugreek seeds revealed a lytic <i>Achromobacter</i> sp., which produces indole acetic acid, has antifungal compounds (e.g., 2-Butanol, 3,3’-oxybis-), and reduces the growth of <i>Rhizoctonia solani</i> by 43.75%. Under the greenhouse and natural field conditions, bacterial cells and/or supernatant improved the growth, physiology, and yield performance of fenugreek plants, and effectively suppressed the progress of root rot disease; this is the first extensive study that uses a new seed-borne endophytic bacterium as a plant-growth-promoting, and biocontrol tool against the sclerotia-forming; <i>R. solani</i>; the causative of fenugreek root rot.https://www.mdpi.com/1420-3049/27/17/5546bacteriaseed endophytes<i>Rhizoctonia solani</i>natural productslytic enzymesplant growth |
| spellingShingle | Ehsan M. Rashad Dalia M. Shaheen Abdulaziz A. Al-Askar Khalid M. Ghoneem Amr Abker Arishi El Sayed A. Hassan WesamEldin I. A. Saber Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek Molecules bacteria seed endophytes <i>Rhizoctonia solani</i> natural products lytic enzymes plant growth |
| title | Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek |
| title_full | Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek |
| title_fullStr | Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek |
| title_full_unstemmed | Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek |
| title_short | Seed Endophytic <i>Achromobacter</i> sp. F23KW as a Promising Growth Promoter and Biocontrol of Rhizoctonia Root Rot of Fenugreek |
| title_sort | seed endophytic i achromobacter i sp f23kw as a promising growth promoter and biocontrol of rhizoctonia root rot of fenugreek |
| topic | bacteria seed endophytes <i>Rhizoctonia solani</i> natural products lytic enzymes plant growth |
| url | https://www.mdpi.com/1420-3049/27/17/5546 |
| work_keys_str_mv | AT ehsanmrashad seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT daliamshaheen seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT abdulazizaalaskar seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT khalidmghoneem seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT amrabkerarishi seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT elsayedahassan seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek AT wesameldiniasaber seedendophyticiachromobacterispf23kwasapromisinggrowthpromoterandbiocontrolofrhizoctoniarootrotoffenugreek |