Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L.
In this work, the total content of phenolics, flavonoids, and phenolic acids in the leaves and bark extracts of Fraxinus ornus L. and Fraxinus excelsior L. was determined. In addition, the identification and quantification of phenolic acids was performed using the HPLC-DAD technique. Samples were te...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2024-01-01
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| Series: | Kemija u Industriji |
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| Online Access: | http://silverstripe.fkit.hr/kui/assets/Uploads/3-19-25-KUI-1-2-2024.pdf |
| _version_ | 1797352821782216704 |
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| author | Amira Čopra-Janićijević Dušan Čulum Danijela Vidic Anela Topčagić Lejla Klepo |
| author_facet | Amira Čopra-Janićijević Dušan Čulum Danijela Vidic Anela Topčagić Lejla Klepo |
| author_sort | Amira Čopra-Janićijević |
| collection | DOAJ |
| description | In this work, the total content of phenolics, flavonoids, and phenolic acids in the leaves and bark extracts of Fraxinus ornus L. and Fraxinus excelsior L. was determined. In addition, the identification and quantification of phenolic acids was performed using the HPLC-DAD technique. Samples were tested for antioxidant activity using the ABTS and DPPH methods. Eight extract samples were prepared by Soxhlet and ultrasound extraction using 70 % ethanol as solvent. The content of phenolic compounds ranged from 7.59 for F. ornus to 88.93 mg GAE/g for F. excelsior in the bark extracts obtained by Soxhlet extraction. The highest content of total flavonoids in F. ornus and F. excelsior was found in the leaves extracts obtained by ultrasound extraction, which was 5.68 and 1.74 mg GAE/g extract, respectively. The results also showed that the highest content of total phenolic acids was found in F. ornus (105.33 mg CAE/g) and F. excelsior (97.97 mg CAE/g) in the bark extract obtained by Soxhlet extraction. The highest content of gallic acid (112.96 ± 1.32 mg g–1 extracts) and chlorogenic acid (246.94 ± 0.82 mg g–1 extracts) was found in the F. ornus bark extract obtained by ultrasound extraction. As for antioxidant activity, the bark extract obtained by Soxhlet extraction of F. ornus showed the best antioxidant activity by ABTS method with IC50 value of 0.062 mg ml–1. The results of the DPPH method show that the leaves extract obtained by Soxhlet extraction had the best antioxidant activity for the F. ornus sample, with an IC50 value of 0.23 mg ml–1. |
| first_indexed | 2024-03-08T13:22:20Z |
| format | Article |
| id | doaj.art-2bca4501b81846c886fedb24ad446a42 |
| institution | Directory Open Access Journal |
| issn | 0022-9830 1334-9090 |
| language | English |
| last_indexed | 2024-03-08T13:22:20Z |
| publishDate | 2024-01-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Kemija u Industriji |
| spelling | doaj.art-2bca4501b81846c886fedb24ad446a422024-01-17T23:48:12ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902024-01-01731-2192510.15255/KUI.2023.021Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L.Amira Čopra-Janićijević0Dušan Čulum1Danijela Vidic2Anela Topčagić3Lejla Klepo4University of Sarajevo, Faculty of Science, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Science, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Science, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Science, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Science, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaIn this work, the total content of phenolics, flavonoids, and phenolic acids in the leaves and bark extracts of Fraxinus ornus L. and Fraxinus excelsior L. was determined. In addition, the identification and quantification of phenolic acids was performed using the HPLC-DAD technique. Samples were tested for antioxidant activity using the ABTS and DPPH methods. Eight extract samples were prepared by Soxhlet and ultrasound extraction using 70 % ethanol as solvent. The content of phenolic compounds ranged from 7.59 for F. ornus to 88.93 mg GAE/g for F. excelsior in the bark extracts obtained by Soxhlet extraction. The highest content of total flavonoids in F. ornus and F. excelsior was found in the leaves extracts obtained by ultrasound extraction, which was 5.68 and 1.74 mg GAE/g extract, respectively. The results also showed that the highest content of total phenolic acids was found in F. ornus (105.33 mg CAE/g) and F. excelsior (97.97 mg CAE/g) in the bark extract obtained by Soxhlet extraction. The highest content of gallic acid (112.96 ± 1.32 mg g–1 extracts) and chlorogenic acid (246.94 ± 0.82 mg g–1 extracts) was found in the F. ornus bark extract obtained by ultrasound extraction. As for antioxidant activity, the bark extract obtained by Soxhlet extraction of F. ornus showed the best antioxidant activity by ABTS method with IC50 value of 0.062 mg ml–1. The results of the DPPH method show that the leaves extract obtained by Soxhlet extraction had the best antioxidant activity for the F. ornus sample, with an IC50 value of 0.23 mg ml–1.http://silverstripe.fkit.hr/kui/assets/Uploads/3-19-25-KUI-1-2-2024.pdffraxinus ornusfraxinus excelsiorphenolic compoundsflavonoidsphenolic acidsabtsdpph |
| spellingShingle | Amira Čopra-Janićijević Dušan Čulum Danijela Vidic Anela Topčagić Lejla Klepo Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. Kemija u Industriji fraxinus ornus fraxinus excelsior phenolic compounds flavonoids phenolic acids abts dpph |
| title | Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. |
| title_full | Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. |
| title_fullStr | Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. |
| title_full_unstemmed | Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. |
| title_short | Chemical Composition and Antioxidant Activity of Fraxinus ornus L. and Fraxinus excelsior L. |
| title_sort | chemical composition and antioxidant activity of fraxinus ornus l and fraxinus excelsior l |
| topic | fraxinus ornus fraxinus excelsior phenolic compounds flavonoids phenolic acids abts dpph |
| url | http://silverstripe.fkit.hr/kui/assets/Uploads/3-19-25-KUI-1-2-2024.pdf |
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