Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions
NUCLEAR FACTOR Y subunit alpha (NF-YA), together with NF-YB and NF-YC, regulates plant growth and development, as well as plant responses to biotic and abiotic stresses. Although extensive studies have examined the functions of NF-YAs in Arabidopsis thaliana, the roles of NF- YAs in Glycinme max are...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1033938/full |
| _version_ | 1811340281008619520 |
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| author | Siyan Ou Siyan Ou Siyan Ou Zhihao Xu Zhihao Xu Zhihao Xu Cuishan Mai Cuishan Mai Cuishan Mai Bodi Li Bodi Li Bodi Li Jinxiang Wang Jinxiang Wang Jinxiang Wang |
| author_facet | Siyan Ou Siyan Ou Siyan Ou Zhihao Xu Zhihao Xu Zhihao Xu Cuishan Mai Cuishan Mai Cuishan Mai Bodi Li Bodi Li Bodi Li Jinxiang Wang Jinxiang Wang Jinxiang Wang |
| author_sort | Siyan Ou |
| collection | DOAJ |
| description | NUCLEAR FACTOR Y subunit alpha (NF-YA), together with NF-YB and NF-YC, regulates plant growth and development, as well as plant responses to biotic and abiotic stresses. Although extensive studies have examined the functions of NF-YAs in Arabidopsis thaliana, the roles of NF- YAs in Glycinme max are poorly understood. In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean. The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively. GmNF-YA8 is localized in the nucleus and plasma membrane. Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis. Exogenous application of gibberellic acid (GA) rescues the delayed flowering phenotype in Arabidopsis overexpressing GmNF-YA8 lines GmNF-YA8OE-05 and GmNF-YA8OE-20. Moreover, quantitative real time PCR (qRT-PCR) verified that overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression, but upregulates GA2ox2 and GA2ox3 that encode enzymes, which inactive bioactive GAs. Consistent with the late flowering phenotype of Arabidopsis trangenic lines that overexpress GmNF-YA8, the transcript levels of flowering-promoting genes AP1, CO, LFY, and SOC1 are reduced. In addition, overexpression of GmNF-YA8 promotes the emergence of lateral root (LR) primordium from epidermis rather than the initiation of LR in low P, and increases the LR density in low nitrogen. Our results provide insights into the roles of GmNF-YA8. |
| first_indexed | 2024-04-13T18:39:34Z |
| format | Article |
| id | doaj.art-2bcc61542ba44db1b677b9d27475c44f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T18:39:34Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2bcc61542ba44db1b677b9d27475c44f2022-12-22T02:34:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10339381033938Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditionsSiyan Ou0Siyan Ou1Siyan Ou2Zhihao Xu3Zhihao Xu4Zhihao Xu5Cuishan Mai6Cuishan Mai7Cuishan Mai8Bodi Li9Bodi Li10Bodi Li11Jinxiang Wang12Jinxiang Wang13Jinxiang Wang14State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaRoot Biology Center & College of Natural Resources and Environment, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaRoot Biology Center & College of Natural Resources and Environment, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaRoot Biology Center & College of Natural Resources and Environment, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaRoot Biology Center & College of Natural Resources and Environment, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaRoot Biology Center & College of Natural Resources and Environment, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, ChinaNUCLEAR FACTOR Y subunit alpha (NF-YA), together with NF-YB and NF-YC, regulates plant growth and development, as well as plant responses to biotic and abiotic stresses. Although extensive studies have examined the functions of NF-YAs in Arabidopsis thaliana, the roles of NF- YAs in Glycinme max are poorly understood. In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean. The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively. GmNF-YA8 is localized in the nucleus and plasma membrane. Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis. Exogenous application of gibberellic acid (GA) rescues the delayed flowering phenotype in Arabidopsis overexpressing GmNF-YA8 lines GmNF-YA8OE-05 and GmNF-YA8OE-20. Moreover, quantitative real time PCR (qRT-PCR) verified that overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression, but upregulates GA2ox2 and GA2ox3 that encode enzymes, which inactive bioactive GAs. Consistent with the late flowering phenotype of Arabidopsis trangenic lines that overexpress GmNF-YA8, the transcript levels of flowering-promoting genes AP1, CO, LFY, and SOC1 are reduced. In addition, overexpression of GmNF-YA8 promotes the emergence of lateral root (LR) primordium from epidermis rather than the initiation of LR in low P, and increases the LR density in low nitrogen. Our results provide insights into the roles of GmNF-YA8.https://www.frontiersin.org/articles/10.3389/fpls.2022.1033938/fullGmNF-YA8floweringphosphorus deficiencylateral rootsgibberellic acid |
| spellingShingle | Siyan Ou Siyan Ou Siyan Ou Zhihao Xu Zhihao Xu Zhihao Xu Cuishan Mai Cuishan Mai Cuishan Mai Bodi Li Bodi Li Bodi Li Jinxiang Wang Jinxiang Wang Jinxiang Wang Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions Frontiers in Plant Science GmNF-YA8 flowering phosphorus deficiency lateral roots gibberellic acid |
| title | Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions |
| title_full | Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions |
| title_fullStr | Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions |
| title_full_unstemmed | Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions |
| title_short | Ectopic expression of GmNF-YA8 in Arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis- and flowering-related genes and promotes lateral root emergence in low phosphorus conditions |
| title_sort | ectopic expression of gmnf ya8 in arabidopsis delays flowering via modulating the expression of gibberellic acid biosynthesis and flowering related genes and promotes lateral root emergence in low phosphorus conditions |
| topic | GmNF-YA8 flowering phosphorus deficiency lateral roots gibberellic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1033938/full |
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