Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart
BackgroundUntil now, few articles have revealed the potential roles of innate lymphoid cells (ILCs) in cardiovascular diseases. However, the infiltration of ILC subsets in ischemic myocardium, the roles of ILC subsets in myocardial infarction (MI) and myocardial ischemia-reperfusion injury (MIRI) an...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1129007/full |
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| author | Shijiu Jiang Shijiu Jiang Shijiu Jiang Shijiu Jiang Yuqi Zheng Yuqi Zheng Yuqi Zheng Bingjie Lv Bingjie Lv Bingjie Lv Shaolin He Shaolin He Shaolin He Wenling Yang Wenling Yang Wenling Yang Boyuan Wang Boyuan Wang Boyuan Wang Jin Zhou Jin Zhou Jin Zhou Shangwei Liu Shangwei Liu Shangwei Liu Dazhu Li Dazhu Li Dazhu Li Jibin Lin Jibin Lin Jibin Lin |
| author_facet | Shijiu Jiang Shijiu Jiang Shijiu Jiang Shijiu Jiang Yuqi Zheng Yuqi Zheng Yuqi Zheng Bingjie Lv Bingjie Lv Bingjie Lv Shaolin He Shaolin He Shaolin He Wenling Yang Wenling Yang Wenling Yang Boyuan Wang Boyuan Wang Boyuan Wang Jin Zhou Jin Zhou Jin Zhou Shangwei Liu Shangwei Liu Shangwei Liu Dazhu Li Dazhu Li Dazhu Li Jibin Lin Jibin Lin Jibin Lin |
| author_sort | Shijiu Jiang |
| collection | DOAJ |
| description | BackgroundUntil now, few articles have revealed the potential roles of innate lymphoid cells (ILCs) in cardiovascular diseases. However, the infiltration of ILC subsets in ischemic myocardium, the roles of ILC subsets in myocardial infarction (MI) and myocardial ischemia-reperfusion injury (MIRI) and the related cellular and molecular mechanisms have not been described with a sufficient level of detail.MethodIn the current study, 8-week-old male C57BL/6J mice were divided into three groups: MI, MIRI and sham group. Single-cell sequencing technology was used to perform dimensionality reduction clustering of ILC to analyze the ILC subset landscape at a single-cell resolution, and finally flow cytometry was used to confirm the existence of the new ILC subsets in different disease groups.ResultsFive ILC subsets were found, including ILC1, ILC2a, ILC2b, ILCdc and ILCt. It is worth noting that ILCdc, ILC2b and ILCt were identified as new ILC subclusters in the heart. The cellular landscapes of ILCs were revealed and signal pathways were predicted. Furthermore, pseudotime trajectory analysis exhibited different ILC statuses and traced related gene expression in normal and ischemic conditions. In addition, we established a ligand–receptor–transcription factor–target gene regulatory network to disclose cell communications among ILC clusters. Moreover, we further revealed the transcriptional features of the ILCdc and ILC2a subsets. Finally, the existence of ILCdc was confirmed by flow cytometry.ConclusionCollectively, by characterizing the spectrums of ILC subclusters, our results provide a new blueprint for understanding ILC subclusters’ roles in myocardial ischemia diseases and further potential treatment targets. |
| first_indexed | 2024-04-09T13:43:46Z |
| format | Article |
| id | doaj.art-2bd0042392914583a80f44082ca51755 |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T13:43:46Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-2bd0042392914583a80f44082ca517552023-05-09T05:44:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-05-011410.3389/fimmu.2023.11290071129007Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heartShijiu Jiang0Shijiu Jiang1Shijiu Jiang2Shijiu Jiang3Yuqi Zheng4Yuqi Zheng5Yuqi Zheng6Bingjie Lv7Bingjie Lv8Bingjie Lv9Shaolin He10Shaolin He11Shaolin He12Wenling Yang13Wenling Yang14Wenling Yang15Boyuan Wang16Boyuan Wang17Boyuan Wang18Jin Zhou19Jin Zhou20Jin Zhou21Shangwei Liu22Shangwei Liu23Shangwei Liu24Dazhu Li25Dazhu Li26Dazhu Li27Jibin Lin28Jibin Lin29Jibin Lin30Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, The First Affiliated Hospital, Shihezi University, Shihezi, Xinjiang, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Provincial Engineering Research Center of Immunological Diagnosis and Therapy for Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaBackgroundUntil now, few articles have revealed the potential roles of innate lymphoid cells (ILCs) in cardiovascular diseases. However, the infiltration of ILC subsets in ischemic myocardium, the roles of ILC subsets in myocardial infarction (MI) and myocardial ischemia-reperfusion injury (MIRI) and the related cellular and molecular mechanisms have not been described with a sufficient level of detail.MethodIn the current study, 8-week-old male C57BL/6J mice were divided into three groups: MI, MIRI and sham group. Single-cell sequencing technology was used to perform dimensionality reduction clustering of ILC to analyze the ILC subset landscape at a single-cell resolution, and finally flow cytometry was used to confirm the existence of the new ILC subsets in different disease groups.ResultsFive ILC subsets were found, including ILC1, ILC2a, ILC2b, ILCdc and ILCt. It is worth noting that ILCdc, ILC2b and ILCt were identified as new ILC subclusters in the heart. The cellular landscapes of ILCs were revealed and signal pathways were predicted. Furthermore, pseudotime trajectory analysis exhibited different ILC statuses and traced related gene expression in normal and ischemic conditions. In addition, we established a ligand–receptor–transcription factor–target gene regulatory network to disclose cell communications among ILC clusters. Moreover, we further revealed the transcriptional features of the ILCdc and ILC2a subsets. Finally, the existence of ILCdc was confirmed by flow cytometry.ConclusionCollectively, by characterizing the spectrums of ILC subclusters, our results provide a new blueprint for understanding ILC subclusters’ roles in myocardial ischemia diseases and further potential treatment targets.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1129007/fullinnate lymphoid cellsmyocardial infarctionmyocardial ischemia-reperfusion injury (MIRI)single-cell RNA sequencingtranscription |
| spellingShingle | Shijiu Jiang Shijiu Jiang Shijiu Jiang Shijiu Jiang Yuqi Zheng Yuqi Zheng Yuqi Zheng Bingjie Lv Bingjie Lv Bingjie Lv Shaolin He Shaolin He Shaolin He Wenling Yang Wenling Yang Wenling Yang Boyuan Wang Boyuan Wang Boyuan Wang Jin Zhou Jin Zhou Jin Zhou Shangwei Liu Shangwei Liu Shangwei Liu Dazhu Li Dazhu Li Dazhu Li Jibin Lin Jibin Lin Jibin Lin Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart Frontiers in Immunology innate lymphoid cells myocardial infarction myocardial ischemia-reperfusion injury (MIRI) single-cell RNA sequencing transcription |
| title | Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| title_full | Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| title_fullStr | Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| title_full_unstemmed | Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| title_short | Single-cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| title_sort | single cell landscape dissecting the transcription and heterogeneity of innate lymphoid cells in ischemic heart |
| topic | innate lymphoid cells myocardial infarction myocardial ischemia-reperfusion injury (MIRI) single-cell RNA sequencing transcription |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1129007/full |
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