A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis
Bracoviruses associate symbiotically with thousands of parasitoid wasp species in the family Braconidae, working as virulence gene vectors, and allowing the development of wasp larvae within hosts. These viruses are composed of multiple DNA circles that are packaged into infective particles, and inj...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-12-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.300280 |
| _version_ | 1818741216193282048 |
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| author | Pedro Heringer Guilherme B. Dias Gustavo C. S. Kuhn |
| author_facet | Pedro Heringer Guilherme B. Dias Gustavo C. S. Kuhn |
| author_sort | Pedro Heringer |
| collection | DOAJ |
| description | Bracoviruses associate symbiotically with thousands of parasitoid wasp species in the family Braconidae, working as virulence gene vectors, and allowing the development of wasp larvae within hosts. These viruses are composed of multiple DNA circles that are packaged into infective particles, and injected together with wasp’s eggs during parasitization. One of the viral segments of Cotesia vestalis bracovirus contains a gene that has been previously described as a helicase of unknown origin. Here, we demonstrate that this gene is a Rep/Helicase from an intact Helitron transposable element that covers the viral segment almost entirely. We also provide evidence that this element underwent at least two horizontal transfers, which appear to have occurred consecutively: first from a Drosophila host ancestor to the genome of the parasitoid wasp C. vestalis and its bracovirus, and then from C. vestalis to a lepidopteran host (Bombyx mori). Our results reinforce the idea of parasitoid wasps as frequent agents of horizontal transfers in eukaryotes. Additionally, this Helitron-bracovirus segment is the first example of a transposable element that effectively became a whole viral circle. |
| first_indexed | 2024-12-18T01:53:06Z |
| format | Article |
| id | doaj.art-2bd0281f031449e3affe2c33a2824cdf |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-18T01:53:06Z |
| publishDate | 2017-12-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-2bd0281f031449e3affe2c33a2824cdf2022-12-21T21:24:58ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362017-12-017123925393510.1534/g3.117.3002809A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalisPedro HeringerGuilherme B. DiasGustavo C. S. KuhnBracoviruses associate symbiotically with thousands of parasitoid wasp species in the family Braconidae, working as virulence gene vectors, and allowing the development of wasp larvae within hosts. These viruses are composed of multiple DNA circles that are packaged into infective particles, and injected together with wasp’s eggs during parasitization. One of the viral segments of Cotesia vestalis bracovirus contains a gene that has been previously described as a helicase of unknown origin. Here, we demonstrate that this gene is a Rep/Helicase from an intact Helitron transposable element that covers the viral segment almost entirely. We also provide evidence that this element underwent at least two horizontal transfers, which appear to have occurred consecutively: first from a Drosophila host ancestor to the genome of the parasitoid wasp C. vestalis and its bracovirus, and then from C. vestalis to a lepidopteran host (Bombyx mori). Our results reinforce the idea of parasitoid wasps as frequent agents of horizontal transfers in eukaryotes. Additionally, this Helitron-bracovirus segment is the first example of a transposable element that effectively became a whole viral circle.http://g3journal.org/lookup/doi/10.1534/g3.117.300280Bracovirustransposable elementHelitronhorizontal transferCotesia |
| spellingShingle | Pedro Heringer Guilherme B. Dias Gustavo C. S. Kuhn A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis G3: Genes, Genomes, Genetics Bracovirus transposable element Helitron horizontal transfer Cotesia |
| title | A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis |
| title_full | A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis |
| title_fullStr | A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis |
| title_full_unstemmed | A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis |
| title_short | A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment in Cotesia vestalis |
| title_sort | horizontally transferred autonomous helitron became a full polydnavirus segment in cotesia vestalis |
| topic | Bracovirus transposable element Helitron horizontal transfer Cotesia |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.300280 |
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