| Summary: | The extraction and characterization of new bioactive plant-derived polysaccharides with the potential for use as functional foods and medicine have attracted much attention. In the present study, A novel acidic polysaccharide (RPP-3a) with a weight-average molecular weight (Mw) of 88,997 Da was isolated from the raspberry pulp. RPP-3a was composed of rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid at a molar ratio of 13.1:28.6:16.8:1.4:6.2:33.9. Structural analysis suggested that the RPP-3a backbone was composed of repeating units of →4)-<i>β</i>-Gal<i>p</i>-(1→3,4)-<i>α</i>-Rha<i>p</i>-(1→[4)-<i>α</i>-GalA<i>p</i>-(1→4)-<i>α</i>-GalA<i>p</i>-(1→]<sub>n</sub> with branches at the C-4 position of rhamnose. The side chain of RPP-3a, containing two branch levels, was comprised of <i>α</i>-Ara<i>f</i>-(1→, →5)-<i>α</i>-Ara<i>f</i>-(1→, →3,5)-<i>α</i>-Ara<i>f</i>-(1→, →3)-<i>β</i>-Gal<i>p</i>-(1→, →3,6)-<i>β</i>-Gal<i>p</i>-(1→, →4)-<i>β</i>-Glc<i>p</i>-(1→, and →2,6)-<i>α</i>-Man<i>p</i>-1→ residues. RPP-3a exhibited moderate reducing power and strong hydroxyl and superoxide anion radical scavenging abilities. RPP-3a significantly promoted the viability of RAW264.7 macrophages by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) at both the expression and transcriptional levels. In summary, the immunostimulatory and antioxidant activities make RPP-3a a viable candidate as a health-beneficial functional dietary supplement.
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