Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study
Endometrial immune cells are essential to support uterine functions across the estrous cycle and in preparation for pregnancy. It has been acknowledged that changes in phenotype and/or numbers of lymphocytes, such as regulatory T cells (Tregs) and NK cells, might result in lower fertility in women a...
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/23/3373 |
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| author | Joanna Jaworska Amanda M. de Mestre Joanna Wiśniewska Bettina Wagner Arkadiusz Nowicki Ilona Kowalczyk-Zięba Izabela Wocławek-Potocka |
| author_facet | Joanna Jaworska Amanda M. de Mestre Joanna Wiśniewska Bettina Wagner Arkadiusz Nowicki Ilona Kowalczyk-Zięba Izabela Wocławek-Potocka |
| author_sort | Joanna Jaworska |
| collection | DOAJ |
| description | Endometrial immune cells are essential to support uterine functions across the estrous cycle and in preparation for pregnancy. It has been acknowledged that changes in phenotype and/or numbers of lymphocytes, such as regulatory T cells (Tregs) and NK cells, might result in lower fertility in women and mice. Little is known about equine endometrial immune cells across the estrous cycle. Here, we compared the populations of endometrial Tregs and NK cells in estrus and diestrus in mares. Endometrial biopsy and blood samples were taken in estrus and diestrus from 11 mares ages 4–12 years. Flow cytometry with anti-CD4, -CD25 and -FOXP3 and anti-NKp46 and -CD3 antibodies was used to determine the populations of Tregs and NK cells, respectively. The concentration of progesterone was measured with chemiluminescence immunoassay. The results were analyzed with paired Student <i>t</i> tests. The mean percentage of endometrial CD4<sup>+</sup>FOXP3<sup>+</sup> Tregs was 13.7 ± 6.2% in diestrus and 14.5 ± 5.9% in estrus, while the mean percentage of endometrial CD4<sup>+</sup>FOXP3<sup>+</sup>CD25<sup>+</sup> Tregs changed from 3.6 ± 2.1% in diestrus to 2 ± 2% in estrus (<i>p</i> = 0.0947). The mean proportion of CD3<sup>−</sup>NKp46<sup>+</sup> lymphocytes in the endometrium was not significantly different, with 6 ± 1% in estrus and 6.5 ± 1.4% in diestrus. There was a large variation in the percentage of NK cells between mares of 2.1–12.7%. This study showed, for the first time, the presence of CD4<sup>+</sup>FOXP3<sup>+</sup>CD25<sup>+</sup> Tregs and CD3<sup>−</sup>NKp46<sup>+</sup> NK cells in the endometrium of non-pregnant cycling mares. The percentage of Tregs, and to a greater extent NK cells, showed large fluctuations between mares. Both Tregs and NK cells might be important for the preparation of the endometrium for semen deposition and pregnancy; however, further research is required. |
| first_indexed | 2024-03-09T17:55:01Z |
| format | Article |
| id | doaj.art-2bd86129e5df4e4b91f500adcbc99b3a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:55:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2bd86129e5df4e4b91f500adcbc99b3a2023-11-24T10:26:03ZengMDPI AGAnimals2076-26152022-11-011223337310.3390/ani12233373Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary StudyJoanna Jaworska0Amanda M. de Mestre1Joanna Wiśniewska2Bettina Wagner3Arkadiusz Nowicki4Ilona Kowalczyk-Zięba5Izabela Wocławek-Potocka6Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, PolandDepartment of Comparative Biomedical Sciences, Royal Veterinary College, Hertfordshire, Hatfield AL9 7TA, UKLaboratory of Cell and Tissue Analysis and Imaging, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, PolandDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USAPrivate Veterinary Practice Arkadiusz Nowicki, 10-684 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, PolandEndometrial immune cells are essential to support uterine functions across the estrous cycle and in preparation for pregnancy. It has been acknowledged that changes in phenotype and/or numbers of lymphocytes, such as regulatory T cells (Tregs) and NK cells, might result in lower fertility in women and mice. Little is known about equine endometrial immune cells across the estrous cycle. Here, we compared the populations of endometrial Tregs and NK cells in estrus and diestrus in mares. Endometrial biopsy and blood samples were taken in estrus and diestrus from 11 mares ages 4–12 years. Flow cytometry with anti-CD4, -CD25 and -FOXP3 and anti-NKp46 and -CD3 antibodies was used to determine the populations of Tregs and NK cells, respectively. The concentration of progesterone was measured with chemiluminescence immunoassay. The results were analyzed with paired Student <i>t</i> tests. The mean percentage of endometrial CD4<sup>+</sup>FOXP3<sup>+</sup> Tregs was 13.7 ± 6.2% in diestrus and 14.5 ± 5.9% in estrus, while the mean percentage of endometrial CD4<sup>+</sup>FOXP3<sup>+</sup>CD25<sup>+</sup> Tregs changed from 3.6 ± 2.1% in diestrus to 2 ± 2% in estrus (<i>p</i> = 0.0947). The mean proportion of CD3<sup>−</sup>NKp46<sup>+</sup> lymphocytes in the endometrium was not significantly different, with 6 ± 1% in estrus and 6.5 ± 1.4% in diestrus. There was a large variation in the percentage of NK cells between mares of 2.1–12.7%. This study showed, for the first time, the presence of CD4<sup>+</sup>FOXP3<sup>+</sup>CD25<sup>+</sup> Tregs and CD3<sup>−</sup>NKp46<sup>+</sup> NK cells in the endometrium of non-pregnant cycling mares. The percentage of Tregs, and to a greater extent NK cells, showed large fluctuations between mares. Both Tregs and NK cells might be important for the preparation of the endometrium for semen deposition and pregnancy; however, further research is required.https://www.mdpi.com/2076-2615/12/23/3373Tregsuterushorsefertilityreproduction |
| spellingShingle | Joanna Jaworska Amanda M. de Mestre Joanna Wiśniewska Bettina Wagner Arkadiusz Nowicki Ilona Kowalczyk-Zięba Izabela Wocławek-Potocka Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study Animals Tregs uterus horse fertility reproduction |
| title | Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study |
| title_full | Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study |
| title_fullStr | Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study |
| title_full_unstemmed | Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study |
| title_short | Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares—A Preliminary Study |
| title_sort | populations of nk cells and regulatory t cells in the endometrium of cycling mares a preliminary study |
| topic | Tregs uterus horse fertility reproduction |
| url | https://www.mdpi.com/2076-2615/12/23/3373 |
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