Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1
Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect au...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Magazine House of Cancer Research on Prevention and Treatment
2022-05-01
|
| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.1202 |
| _version_ | 1811232463065710592 |
|---|---|
| author | GAO Haining BAI Ruixia ZHAO Pengwei SONG Wanying LIN Xuan |
| author_facet | GAO Haining BAI Ruixia ZHAO Pengwei SONG Wanying LIN Xuan |
| author_sort | GAO Haining |
| collection | DOAJ |
| description | Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1. |
| first_indexed | 2024-04-12T11:03:12Z |
| format | Article |
| id | doaj.art-2bda2b8e97fb44c4b15ff863e7b24bdd |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-04-12T11:03:12Z |
| publishDate | 2022-05-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-2bda2b8e97fb44c4b15ff863e7b24bdd2022-12-22T03:35:52ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782022-05-0149540340710.3971/j.issn.1000-8578.2022.21.12028578.2022.21.1202Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1GAO Haining0BAI Ruixia1ZHAO Pengwei2SONG Wanying3LIN Xuan4Inner Mongolia Medical College, Inner Mongolia Medical University, Hohhot 010000, ChinaDepartment of Clinical Laboratory, Inner Mongolia People's Hospital, Hohhot 010000, ChinaGraduate School, Inner Mongolia Medical University, Hohhot 010000, ChinaDepartment of Clinical Laboratory, Xing' anmeng People' s Hospital, Xing' anmeng 137400, ChinaInner Mongolia Medical College, Inner Mongolia Medical University, Hohhot 010000, ChinaObjective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.1202ovarian cancerskov3 cellsautophagymir-581foxo1 |
| spellingShingle | GAO Haining BAI Ruixia ZHAO Pengwei SONG Wanying LIN Xuan Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 Zhongliu Fangzhi Yanjiu ovarian cancer skov3 cells autophagy mir-581 foxo1 |
| title | Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
| title_full | Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
| title_fullStr | Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
| title_full_unstemmed | Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
| title_short | Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
| title_sort | effect of mir 581 on autophagy of ovarian cancer skov3 cells by regulating foxo1 |
| topic | ovarian cancer skov3 cells autophagy mir-581 foxo1 |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.1202 |
| work_keys_str_mv | AT gaohaining effectofmir581onautophagyofovariancancerskov3cellsbyregulatingfoxo1 AT bairuixia effectofmir581onautophagyofovariancancerskov3cellsbyregulatingfoxo1 AT zhaopengwei effectofmir581onautophagyofovariancancerskov3cellsbyregulatingfoxo1 AT songwanying effectofmir581onautophagyofovariancancerskov3cellsbyregulatingfoxo1 AT linxuan effectofmir581onautophagyofovariancancerskov3cellsbyregulatingfoxo1 |