Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model
AbstractObjective Comparison of hormonal, metabolic and inflammatory markers of glutathione with metformin and Diane-35 in a rat model of PCOS induced by dehydroepiandrosterone.Methods Twenty-five female rats were randomized into four groups. Group 1 was administered a subcutaneous dose of 0.2 ml sa...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Gynecological Endocrinology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09513590.2024.2302086 |
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| author | Aslı Coşar Pınar Özcan Fatma Basak Tanoglu Olgu Enis Tok Gülçin Özkara Hikmet Tunç Timur Çağlar Çetin Duygu Neccar |
| author_facet | Aslı Coşar Pınar Özcan Fatma Basak Tanoglu Olgu Enis Tok Gülçin Özkara Hikmet Tunç Timur Çağlar Çetin Duygu Neccar |
| author_sort | Aslı Coşar |
| collection | DOAJ |
| description | AbstractObjective Comparison of hormonal, metabolic and inflammatory markers of glutathione with metformin and Diane-35 in a rat model of PCOS induced by dehydroepiandrosterone.Methods Twenty-five female rats were randomized into four groups. Group 1 was administered a subcutaneous dose of 0.2 ml saline/day. Group 2 was given 0.2 ml of 1% carboxymethyl cellulose (CMC)/day orally for 28 days. A PCOS model was established with DHEA in rats. Group 3 was given 4.5 mg/kg/day of Diane-35 orally dissolved in 1% CMC for 28 days. Group 4 was given 300 mg/kg/day of metformin orally dissolved in 1 ml of saline for 28 days, and Group 5 was administered 100 mg/kg of glutathione intraperitoneally on days 35, 42, and 49. On day 56, the rats were sacrificed. Serum markers and follicle count were examined.Results Serum IL-6, hs-CRP, insulin, testosterone, SHBG, and MDA values were significantly lower in the glutathione group than in the PCOS group (p = 0.0006, p = 0.023, p = 0.0082, p = 0.0007, p = 0.0048, and p < 0.0001, respectively).The number of all follicles was similar between the control and glutathione groups (p < 0.05). When we compared the other groups with the PCOS group, the number of primary, secondary, atretic, and cystic follicles was significantly lower in the metformin and glutathione groups. The number of primordial and antral follicles was significantly higher than in the PCOS group.Conclusions Glutathione plays anti-inflammatory and antioxidant roles, similar to metformin, by lowering serum IL-6, insulin, testosterone, CRP, and MDA levels; decreasing atretic/cystic follicle count; and improving antral follicle count and folliculogenesis in PCOS patients. |
| first_indexed | 2024-03-08T12:07:28Z |
| format | Article |
| id | doaj.art-2bdafff97499487c884951121f0ce436 |
| institution | Directory Open Access Journal |
| issn | 0951-3590 1473-0766 |
| language | English |
| last_indexed | 2024-03-08T12:07:28Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gynecological Endocrinology |
| spelling | doaj.art-2bdafff97499487c884951121f0ce4362024-01-23T05:42:03ZengTaylor & Francis GroupGynecological Endocrinology0951-35901473-07662024-12-0140110.1080/09513590.2024.2302086Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat modelAslı Coşar0Pınar Özcan1Fatma Basak Tanoglu2Olgu Enis Tok3Gülçin Özkara4Hikmet Tunç Timur5Çağlar Çetin6Duygu Neccar7Department of Obstetrics and Gynecology, Bezmialem University School of Medicine, Istanbul, TurkiyeDepartment of Obstetrics and Gynecology, Bezmialem University School of Medicine, Istanbul, TurkiyeDepartment of Obstetrics and Gynecology, Bezmialem University School of Medicine, Istanbul, TurkiyeSchool of Medicine and Research Institute for Health Sciences and Technologies, Department of Histology and Embryology, Istanbul Medipol University, Istanbul, TurkiyeDepartment of Medical Biology, Bezmialem University School of Medicine, Istanbul, TurkiyeDeparment of Obstetrics and Gynecology, Dokuz Eylul University School of Medicine, Izmir, TurkiyeDepartment of Obstetrics and Gynecology, Bezmialem University School of Medicine, Istanbul, TurkiyeDepartment of Histology and Embryology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, TurkiyeAbstractObjective Comparison of hormonal, metabolic and inflammatory markers of glutathione with metformin and Diane-35 in a rat model of PCOS induced by dehydroepiandrosterone.Methods Twenty-five female rats were randomized into four groups. Group 1 was administered a subcutaneous dose of 0.2 ml saline/day. Group 2 was given 0.2 ml of 1% carboxymethyl cellulose (CMC)/day orally for 28 days. A PCOS model was established with DHEA in rats. Group 3 was given 4.5 mg/kg/day of Diane-35 orally dissolved in 1% CMC for 28 days. Group 4 was given 300 mg/kg/day of metformin orally dissolved in 1 ml of saline for 28 days, and Group 5 was administered 100 mg/kg of glutathione intraperitoneally on days 35, 42, and 49. On day 56, the rats were sacrificed. Serum markers and follicle count were examined.Results Serum IL-6, hs-CRP, insulin, testosterone, SHBG, and MDA values were significantly lower in the glutathione group than in the PCOS group (p = 0.0006, p = 0.023, p = 0.0082, p = 0.0007, p = 0.0048, and p < 0.0001, respectively).The number of all follicles was similar between the control and glutathione groups (p < 0.05). When we compared the other groups with the PCOS group, the number of primary, secondary, atretic, and cystic follicles was significantly lower in the metformin and glutathione groups. The number of primordial and antral follicles was significantly higher than in the PCOS group.Conclusions Glutathione plays anti-inflammatory and antioxidant roles, similar to metformin, by lowering serum IL-6, insulin, testosterone, CRP, and MDA levels; decreasing atretic/cystic follicle count; and improving antral follicle count and folliculogenesis in PCOS patients.https://www.tandfonline.com/doi/10.1080/09513590.2024.2302086GlutathionepCOSmetformindHEAantioxidant |
| spellingShingle | Aslı Coşar Pınar Özcan Fatma Basak Tanoglu Olgu Enis Tok Gülçin Özkara Hikmet Tunç Timur Çağlar Çetin Duygu Neccar Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model Gynecological Endocrinology Glutathione pCOS metformin dHEA antioxidant |
| title | Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model |
| title_full | Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model |
| title_fullStr | Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model |
| title_full_unstemmed | Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model |
| title_short | Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model |
| title_sort | comparative effects of the antioxidant glutathione with metformin and diane 35 on hormonal metabolic and inflammatory indicators in a dhea induced pcos rat model |
| topic | Glutathione pCOS metformin dHEA antioxidant |
| url | https://www.tandfonline.com/doi/10.1080/09513590.2024.2302086 |
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