Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells
<i>Background and objectives:</i> Enamel matrix derivative (EMD) is produced from developing porcine tooth buds and represents a complex of low-molecular-weight hydrophobic enamel proteins. EMD is widely applied in periodontal regeneration. Osteoclasts are multinuclear cells, which are r...
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MDPI AG
2021-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/57/3/269 |
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| author | Gerlinde Durstberger Phuong Quynh Nguyen Verena Hohensinner Peter Pietschmann Xiaohui Rausch-Fan Oleh Andrukhov |
| author_facet | Gerlinde Durstberger Phuong Quynh Nguyen Verena Hohensinner Peter Pietschmann Xiaohui Rausch-Fan Oleh Andrukhov |
| author_sort | Gerlinde Durstberger |
| collection | DOAJ |
| description | <i>Background and objectives:</i> Enamel matrix derivative (EMD) is produced from developing porcine tooth buds and represents a complex of low-molecular-weight hydrophobic enamel proteins. EMD is widely applied in periodontal regeneration. Osteoclasts are multinuclear cells, which are responsible for bone resorption. The precursors of osteoclasts, hematopoietic cells, undergo in vivo the process of transendothelial migration before differentiation. EMD is known to affect the process of osteoclastogenesis, but its effect on human osteoclasts precursors after the interaction with activated endothelium was never studied. <i>Materials and Methods:</i> Human umbilical vein endothelial cells (HUVECs)s were seeded in transwell inserts with a pore size of 8 µm and pre-activated by TNF-α and IL-1β for 18 h. Peripheral blood mononuclear cells (PBMCs), freshly isolated from 16 periodontitis patients and 16 healthy individuals, were added to pre-activated HUVECs. Adherent, non-adherent and transmigrated cells were collected and differentiated to osteoclasts by the standard protocol in the presence or absence of EMD. The number of osteoclasts was determined by tartrate-resistant acid phosphatase staining. <i>Results:</i> PBMCs isolated from periodontitis patients have formed a significantly higher osteoclast number compared to PBMCs isolated from healthy individuals (<i>p</i> < 0.05). EMD induced concentration-dependent inhibition of osteoclast formation from PBMCs. This was true for the different PBMC fractions isolated from both healthy individuals and periodontitis patients. <i>Conclusions:</i> Our data show that EMD inhibits the formation and activity of osteoclasts differentiated from the progenitor cells after the interaction with activated endothelium. This might be associated with bone resorption inhibition and supporting bone regeneration in the frame of periodontal therapy. |
| first_indexed | 2024-03-10T13:12:20Z |
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| language | English |
| last_indexed | 2024-03-10T13:12:20Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-2bdc9b6480c6407aab29a2c0ad92696d2023-11-21T10:36:52ZengMDPI AGMedicina1010-660X1648-91442021-03-0157326910.3390/medicina57030269Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial CellsGerlinde Durstberger0Phuong Quynh Nguyen1Verena Hohensinner2Peter Pietschmann3Xiaohui Rausch-Fan4Oleh Andrukhov5Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaCompetence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immuology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immuology, Medical University of Vienna, 1090 Vienna, AustriaDivision of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaCompetence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria<i>Background and objectives:</i> Enamel matrix derivative (EMD) is produced from developing porcine tooth buds and represents a complex of low-molecular-weight hydrophobic enamel proteins. EMD is widely applied in periodontal regeneration. Osteoclasts are multinuclear cells, which are responsible for bone resorption. The precursors of osteoclasts, hematopoietic cells, undergo in vivo the process of transendothelial migration before differentiation. EMD is known to affect the process of osteoclastogenesis, but its effect on human osteoclasts precursors after the interaction with activated endothelium was never studied. <i>Materials and Methods:</i> Human umbilical vein endothelial cells (HUVECs)s were seeded in transwell inserts with a pore size of 8 µm and pre-activated by TNF-α and IL-1β for 18 h. Peripheral blood mononuclear cells (PBMCs), freshly isolated from 16 periodontitis patients and 16 healthy individuals, were added to pre-activated HUVECs. Adherent, non-adherent and transmigrated cells were collected and differentiated to osteoclasts by the standard protocol in the presence or absence of EMD. The number of osteoclasts was determined by tartrate-resistant acid phosphatase staining. <i>Results:</i> PBMCs isolated from periodontitis patients have formed a significantly higher osteoclast number compared to PBMCs isolated from healthy individuals (<i>p</i> < 0.05). EMD induced concentration-dependent inhibition of osteoclast formation from PBMCs. This was true for the different PBMC fractions isolated from both healthy individuals and periodontitis patients. <i>Conclusions:</i> Our data show that EMD inhibits the formation and activity of osteoclasts differentiated from the progenitor cells after the interaction with activated endothelium. This might be associated with bone resorption inhibition and supporting bone regeneration in the frame of periodontal therapy.https://www.mdpi.com/1648-9144/57/3/269periodontitisenamel matrix derivativeosteoclast |
| spellingShingle | Gerlinde Durstberger Phuong Quynh Nguyen Verena Hohensinner Peter Pietschmann Xiaohui Rausch-Fan Oleh Andrukhov Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells Medicina periodontitis enamel matrix derivative osteoclast |
| title | Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells |
| title_full | Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells |
| title_fullStr | Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells |
| title_full_unstemmed | Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells |
| title_short | Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells |
| title_sort | effect of enamel matrix derivatives on osteoclast formation from pbmc of periodontitis patients and healthy individuals after interaction with activated endothelial cells |
| topic | periodontitis enamel matrix derivative osteoclast |
| url | https://www.mdpi.com/1648-9144/57/3/269 |
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