Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons.
Following injury, dorsal root ganglion (DRG) neurons undergo transcriptional changes so as to adopt phenotypic changes that promote cell survival and axonal regeneration. Here we used a microarray approach to profile changes in a population of small noncoding RNAs known as microRNAs (miRNAs) in the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3154476?pdf=render |
| _version_ | 1819092107481055232 |
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| author | Iain T Strickland Louise Richards Fiona E Holmes David Wynick James B Uney Liang-Fong Wong |
| author_facet | Iain T Strickland Louise Richards Fiona E Holmes David Wynick James B Uney Liang-Fong Wong |
| author_sort | Iain T Strickland |
| collection | DOAJ |
| description | Following injury, dorsal root ganglion (DRG) neurons undergo transcriptional changes so as to adopt phenotypic changes that promote cell survival and axonal regeneration. Here we used a microarray approach to profile changes in a population of small noncoding RNAs known as microRNAs (miRNAs) in the L4 and L5 DRG following sciatic nerve transection. Results showed that 20 miRNA transcripts displayed a significant change in expression levels, with 8 miRNAs transcripts being altered by more than 1.5-fold. Using quantitative reverse transcription PCR, we demonstrated that one of these miRNAs, miR-21, was upregulated by 7-fold in the DRG at 7 days post-axotomy. In dissociated adult rat DRG neurons lentiviral vector-mediated overexpression of miR-21 promoted neurite outgrowth on a reduced laminin substrate. miR-21 directly downregulated expression of Sprouty2 protein, as confirmed by Western blot analysis and 3' untranslated region (UTR) luciferase assays. Our data show that miR-21 is an axotomy-induced miRNA that enhances axon growth, and suggest that miRNAs are important players in regulating growth pathways following peripheral nerve injury. |
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| format | Article |
| id | doaj.art-2bdd339e37c14dd6ac0b92a241ba6ed7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:50:22Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-2bdd339e37c14dd6ac0b92a241ba6ed72022-12-21T18:47:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2342310.1371/journal.pone.0023423Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons.Iain T StricklandLouise RichardsFiona E HolmesDavid WynickJames B UneyLiang-Fong WongFollowing injury, dorsal root ganglion (DRG) neurons undergo transcriptional changes so as to adopt phenotypic changes that promote cell survival and axonal regeneration. Here we used a microarray approach to profile changes in a population of small noncoding RNAs known as microRNAs (miRNAs) in the L4 and L5 DRG following sciatic nerve transection. Results showed that 20 miRNA transcripts displayed a significant change in expression levels, with 8 miRNAs transcripts being altered by more than 1.5-fold. Using quantitative reverse transcription PCR, we demonstrated that one of these miRNAs, miR-21, was upregulated by 7-fold in the DRG at 7 days post-axotomy. In dissociated adult rat DRG neurons lentiviral vector-mediated overexpression of miR-21 promoted neurite outgrowth on a reduced laminin substrate. miR-21 directly downregulated expression of Sprouty2 protein, as confirmed by Western blot analysis and 3' untranslated region (UTR) luciferase assays. Our data show that miR-21 is an axotomy-induced miRNA that enhances axon growth, and suggest that miRNAs are important players in regulating growth pathways following peripheral nerve injury.http://europepmc.org/articles/PMC3154476?pdf=render |
| spellingShingle | Iain T Strickland Louise Richards Fiona E Holmes David Wynick James B Uney Liang-Fong Wong Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. PLoS ONE |
| title | Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. |
| title_full | Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. |
| title_fullStr | Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. |
| title_full_unstemmed | Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. |
| title_short | Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. |
| title_sort | axotomy induced mir 21 promotes axon growth in adult dorsal root ganglion neurons |
| url | http://europepmc.org/articles/PMC3154476?pdf=render |
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