Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice
Abstract Background Idiopathic pulmonary fibrosis is characterized by loss of lung epithelial cells and inexorable progression of fibrosis with no effective and approved treatments. The distal airway stem/progenitor cells (DASCs) have been shown to have potent regenerative capacity after lung injury...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-019-1257-2 |
| _version_ | 1819073310529421312 |
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| author | Yun Shi Mingqing Dong Yueqing Zhou Wangping Li Yongheng Gao Luyao Han Min Chen Hongwei Lin Wei Zuo Faguang Jin |
| author_facet | Yun Shi Mingqing Dong Yueqing Zhou Wangping Li Yongheng Gao Luyao Han Min Chen Hongwei Lin Wei Zuo Faguang Jin |
| author_sort | Yun Shi |
| collection | DOAJ |
| description | Abstract Background Idiopathic pulmonary fibrosis is characterized by loss of lung epithelial cells and inexorable progression of fibrosis with no effective and approved treatments. The distal airway stem/progenitor cells (DASCs) have been shown to have potent regenerative capacity after lung injury. In this work, we aimed to define the role of mouse DASCs (mDASCs) in response to bleomycin-induced lung fibrosis in mice. Methods The mDASCs were isolated, expanded in vitro, and labeled with GFP by lentiviral infection. The labeled mDASCs were intratracheally instilled into bleomycin-induced pulmonary fibrosis mice on day 7. Pathological change, collagen content, α-SMA expression, lung function, and mortality rate were assessed at 7, 14, and 21 days after bleomycin administration. Tissue section and direct fluorescence staining was used to show the distribution and differentiation of mDASCs in lung. Results The transplanted mDASCs could incorporate, proliferate, and differentiate into type I pneumocytes in bleomycin-injured lung. They also inhibited fibrogenesis by attenuating the deposition of collagen and expression of α-SMA. In addition, mDASCs improved pulmonary function and reduce mortality in bleomycin-induced pulmonary fibrosis mice. Conclusions The data strongly suggest that mDASCs could ameliorate bleomycin-induced pulmonary fibrosis by promotion of lung regeneration and inhibition of lung fibrogenesis. |
| first_indexed | 2024-12-21T17:51:35Z |
| format | Article |
| id | doaj.art-2bdde017cf864db8949a9a292cb239a7 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-21T17:51:35Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-2bdde017cf864db8949a9a292cb239a72022-12-21T18:55:19ZengBMCStem Cell Research & Therapy1757-65122019-06-0110111110.1186/s13287-019-1257-2Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in miceYun Shi0Mingqing Dong1Yueqing Zhou2Wangping Li3Yongheng Gao4Luyao Han5Min Chen6Hongwei Lin7Wei Zuo8Faguang Jin9Department of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityXi’an International UniversityShanghai East Hospital, School of Medicine, Tongji UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityShanghai East Hospital, School of Medicine, Tongji UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityAbstract Background Idiopathic pulmonary fibrosis is characterized by loss of lung epithelial cells and inexorable progression of fibrosis with no effective and approved treatments. The distal airway stem/progenitor cells (DASCs) have been shown to have potent regenerative capacity after lung injury. In this work, we aimed to define the role of mouse DASCs (mDASCs) in response to bleomycin-induced lung fibrosis in mice. Methods The mDASCs were isolated, expanded in vitro, and labeled with GFP by lentiviral infection. The labeled mDASCs were intratracheally instilled into bleomycin-induced pulmonary fibrosis mice on day 7. Pathological change, collagen content, α-SMA expression, lung function, and mortality rate were assessed at 7, 14, and 21 days after bleomycin administration. Tissue section and direct fluorescence staining was used to show the distribution and differentiation of mDASCs in lung. Results The transplanted mDASCs could incorporate, proliferate, and differentiate into type I pneumocytes in bleomycin-injured lung. They also inhibited fibrogenesis by attenuating the deposition of collagen and expression of α-SMA. In addition, mDASCs improved pulmonary function and reduce mortality in bleomycin-induced pulmonary fibrosis mice. Conclusions The data strongly suggest that mDASCs could ameliorate bleomycin-induced pulmonary fibrosis by promotion of lung regeneration and inhibition of lung fibrogenesis.http://link.springer.com/article/10.1186/s13287-019-1257-2Pulmonary fibrosisDistal airway stem/progenitor cellsBleomycin |
| spellingShingle | Yun Shi Mingqing Dong Yueqing Zhou Wangping Li Yongheng Gao Luyao Han Min Chen Hongwei Lin Wei Zuo Faguang Jin Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice Stem Cell Research & Therapy Pulmonary fibrosis Distal airway stem/progenitor cells Bleomycin |
| title | Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice |
| title_full | Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice |
| title_fullStr | Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice |
| title_full_unstemmed | Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice |
| title_short | Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice |
| title_sort | distal airway stem cells ameliorate bleomycin induced pulmonary fibrosis in mice |
| topic | Pulmonary fibrosis Distal airway stem/progenitor cells Bleomycin |
| url | http://link.springer.com/article/10.1186/s13287-019-1257-2 |
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