Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha
The aim of this research is to investigate the influence of oleic acid concentration on the cell growth and the physical properties of the polymer formed by cultures of Ralstonia eutropha in mineral medium. Cells were cultivated in Erlenmeyer flasks with 300 mL of mineral medium, containing glucose...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2008-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/38489 |
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| author | Vitor Henrique Grigull Delne Domingos da Silva Michele Cristina Formolo Garcia Sandra Aparecida Furlan Ana Paula Testa Pezzin Gláucia Falcão Aragão Andréa Lima dos Santos Schneider |
| author_facet | Vitor Henrique Grigull Delne Domingos da Silva Michele Cristina Formolo Garcia Sandra Aparecida Furlan Ana Paula Testa Pezzin Gláucia Falcão Aragão Andréa Lima dos Santos Schneider |
| author_sort | Vitor Henrique Grigull |
| collection | DOAJ |
| description | The aim of this research is to investigate the influence of oleic acid concentration on the cell growth and the physical properties of the polymer formed by cultures of Ralstonia eutropha in mineral medium. Cells were cultivated in Erlenmeyer flasks with 300 mL of mineral medium, containing glucose and fructose as a carbon source (30 g/L) and ammonium sulphate (5.0 g/L) as a nitrogen source. Oleic acid was added as nutritional supplement in different concentrations (0, 0.3, 0.9, 1.5 and 3.0 g/L) and the cells were incubated at 30 °C and 150 rpm. The films prepared by casting were evaluated by X-ray diffraction, thermogravimetry and differential scanning calorimetry. These results indicate that the increase of oleic acid concentrations leads to a higher specific growth rate and cell productivity. The characterization of the films revealed that the increase of the concentration of oleic acid from 0 to 1.5 g/L has no influence on thermal behaviour and crystallinity degree. However, the thermal stability, melting temperature, glass transition temperatures and crystallinity degree decreased when 3.0 g/L of oleic acid were used. |
| first_indexed | 2024-03-09T07:47:42Z |
| format | Article |
| id | doaj.art-2be26643d0c7490085e2b3cfe9e2523a |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:47:42Z |
| publishDate | 2008-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-2be26643d0c7490085e2b3cfe9e2523a2023-12-03T02:42:10ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062008-01-01462223228Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutrophaVitor Henrique Grigull0Delne Domingos da Silva1Michele Cristina Formolo Garcia2Sandra Aparecida Furlan3Ana Paula Testa Pezzin4Gláucia Falcão Aragão5Andréa Lima dos Santos Schneider6Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilLaboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilLaboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilLaboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilLaboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilFederal University of Santa Catarina, Florianópolis, BR-88800-000 BrazilLaboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 BrazilThe aim of this research is to investigate the influence of oleic acid concentration on the cell growth and the physical properties of the polymer formed by cultures of Ralstonia eutropha in mineral medium. Cells were cultivated in Erlenmeyer flasks with 300 mL of mineral medium, containing glucose and fructose as a carbon source (30 g/L) and ammonium sulphate (5.0 g/L) as a nitrogen source. Oleic acid was added as nutritional supplement in different concentrations (0, 0.3, 0.9, 1.5 and 3.0 g/L) and the cells were incubated at 30 °C and 150 rpm. The films prepared by casting were evaluated by X-ray diffraction, thermogravimetry and differential scanning calorimetry. These results indicate that the increase of oleic acid concentrations leads to a higher specific growth rate and cell productivity. The characterization of the films revealed that the increase of the concentration of oleic acid from 0 to 1.5 g/L has no influence on thermal behaviour and crystallinity degree. However, the thermal stability, melting temperature, glass transition temperatures and crystallinity degree decreased when 3.0 g/L of oleic acid were used.http://hrcak.srce.hr/file/38489biopolymersRalstonia eutrophaoleic acidcharacterization |
| spellingShingle | Vitor Henrique Grigull Delne Domingos da Silva Michele Cristina Formolo Garcia Sandra Aparecida Furlan Ana Paula Testa Pezzin Gláucia Falcão Aragão Andréa Lima dos Santos Schneider Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha Food Technology and Biotechnology biopolymers Ralstonia eutropha oleic acid characterization |
| title | Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha |
| title_full | Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha |
| title_fullStr | Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha |
| title_full_unstemmed | Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha |
| title_short | Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha |
| title_sort | production and characterization of poly 3 hydroxybutyrate from oleic acid by ralstonia eutropha |
| topic | biopolymers Ralstonia eutropha oleic acid characterization |
| url | http://hrcak.srce.hr/file/38489 |
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