Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China
The emergence and prevalence of multidrug-resistant Acinetobacter baumannii (MRAB) poses a huge challenge to clinical treatment. To investigate the genetic and epidemiologic characteristics of MRAB in Liaocheng, China, and to explore potential resistance mechanisms, 56 MRAB strains were collected fr...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-06-01
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| Series: | Journal of Biosafety and Biosecurity |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2588933823000213 |
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| author | Fangyuan Cui Li Liu Xuefeng Miao Haiying Qian Shaocai Lu Jinjing Tian Guanhua Qiao Baobin Shao Qian Li Ran Zhang Shengnan Liang |
| author_facet | Fangyuan Cui Li Liu Xuefeng Miao Haiying Qian Shaocai Lu Jinjing Tian Guanhua Qiao Baobin Shao Qian Li Ran Zhang Shengnan Liang |
| author_sort | Fangyuan Cui |
| collection | DOAJ |
| description | The emergence and prevalence of multidrug-resistant Acinetobacter baumannii (MRAB) poses a huge challenge to clinical treatment. To investigate the genetic and epidemiologic characteristics of MRAB in Liaocheng, China, and to explore potential resistance mechanisms, 56 MRAB strains were collected from the clinical departments of seven hospitals in Liaocheng between 2020 and 2022. Molecular typing, antimicrobial resistance patterns, and epidemiological characteristics were determined by genome sequencing and comparative genome analysis. Sequence type (ST) 540 was the most prevalent ST of the 56 MRAB in Liaocheng, and most strains (92.86%) were grouped into CC92. Core genome multilocus sequence typing subdivided the strains according to the number of allelic differences and could distinguish different outbreaks caused by ST540 isolates in the hospitals. All the isolates harbored blaOXA-23 and blaADC-25, and at least 92.86% of the isolates were resistant to 10 antibiotics. The major MRAB epidemic clone detected in Liaocheng was ST540, which was different from the results reported in other regions in China. Furthermore, several inter-hospital transmissions of ST540 isolates were observed. The findings highlight the urgent need for effective nosocomial infection control measures and the continuous surveillance of ST540 MRAB in Liaocheng. |
| first_indexed | 2024-03-12T23:38:30Z |
| format | Article |
| id | doaj.art-2be399f906394f6b9eed38fc55a74b91 |
| institution | Directory Open Access Journal |
| issn | 2588-9338 |
| language | English |
| last_indexed | 2024-03-12T23:38:30Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Biosafety and Biosecurity |
| spelling | doaj.art-2be399f906394f6b9eed38fc55a74b912023-07-15T04:29:04ZengKeAi Communications Co., Ltd.Journal of Biosafety and Biosecurity2588-93382023-06-01526066Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, ChinaFangyuan Cui0Li Liu1Xuefeng Miao2Haiying Qian3Shaocai Lu4Jinjing Tian5Guanhua Qiao6Baobin Shao7Qian Li8Ran Zhang9Shengnan Liang10Liaocheng Center for Disease Control and Prevention, Liaocheng 252000, Shandong, ChinaLiaocheng Center for Disease Control and Prevention, Liaocheng 252000, Shandong, ChinaLiaocheng Center for Disease Control and Prevention, Liaocheng 252000, Shandong, ChinaDepartment of Laboratory, Liaocheng Third People’s Hospital, Liaocheng 252000, Shandong, ChinaDepartment of Laboratory, Shenxian People’s Hospital, Liaocheng 252400, Shandong, ChinaDepartment of Laboratory, The Second People’s Hospital of Liaocheng, Liaocheng 252600, Shandong, ChinaDepartment of Laboratory, Linqing People’s Hospital, Liaocheng 252600, Shandong, ChinaDepartment of Laboratory, Donge People’s Hospital, Liaocheng 252200, Shandong, ChinaDepartment of Laboratory, Liaocheng Women’s and Children’s Hospital, Liaocheng 252000, Shandong, ChinaDepartment of Laboratory, Liaocheng People’s Hospital, Liaocheng 252000, Shandong, ChinaLiaocheng Center for Disease Control and Prevention, Liaocheng 252000, Shandong, China; Corresponding author.The emergence and prevalence of multidrug-resistant Acinetobacter baumannii (MRAB) poses a huge challenge to clinical treatment. To investigate the genetic and epidemiologic characteristics of MRAB in Liaocheng, China, and to explore potential resistance mechanisms, 56 MRAB strains were collected from the clinical departments of seven hospitals in Liaocheng between 2020 and 2022. Molecular typing, antimicrobial resistance patterns, and epidemiological characteristics were determined by genome sequencing and comparative genome analysis. Sequence type (ST) 540 was the most prevalent ST of the 56 MRAB in Liaocheng, and most strains (92.86%) were grouped into CC92. Core genome multilocus sequence typing subdivided the strains according to the number of allelic differences and could distinguish different outbreaks caused by ST540 isolates in the hospitals. All the isolates harbored blaOXA-23 and blaADC-25, and at least 92.86% of the isolates were resistant to 10 antibiotics. The major MRAB epidemic clone detected in Liaocheng was ST540, which was different from the results reported in other regions in China. Furthermore, several inter-hospital transmissions of ST540 isolates were observed. The findings highlight the urgent need for effective nosocomial infection control measures and the continuous surveillance of ST540 MRAB in Liaocheng.http://www.sciencedirect.com/science/article/pii/S2588933823000213Acinetobacter baumanniicgMLSTMultidrug resistanceEpidemiological characteristicsGenome analysis and comparison |
| spellingShingle | Fangyuan Cui Li Liu Xuefeng Miao Haiying Qian Shaocai Lu Jinjing Tian Guanhua Qiao Baobin Shao Qian Li Ran Zhang Shengnan Liang Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China Journal of Biosafety and Biosecurity Acinetobacter baumannii cgMLST Multidrug resistance Epidemiological characteristics Genome analysis and comparison |
| title | Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China |
| title_full | Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China |
| title_fullStr | Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China |
| title_full_unstemmed | Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China |
| title_short | Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China |
| title_sort | epidemiological and comparative genomic analyses of multidrug resistant acinetobacter baumannii collected between 2020 and 2022 in liaocheng city shandong province china |
| topic | Acinetobacter baumannii cgMLST Multidrug resistance Epidemiological characteristics Genome analysis and comparison |
| url | http://www.sciencedirect.com/science/article/pii/S2588933823000213 |
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